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EC number: 291-813-1 | CAS number: 90480-76-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 19 February 2018 - 30 March 2018
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)
- Version / remarks:
- 2006
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: Control, 0.010, 0.032, 0.10, 0.32, 1.0 mg/L
- Sample storage conditions before analysis: The samples were stored frozen prior to analysis. - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
Due to the need to test at relatively low test concentrations a single WAF of a nominal loading rate of 1.0 mg/L was prepared from which a series of dilutions was made to give the remaining test concentrations.
A nominal amount of test item (20 mg) was added to the surface of 20 liters of culture medium to give the 1.0 mg/L loading rate. After the addition of the test item, the culture medium was stirred by magnetic stirrer using a stirring rate such that a vortex was formed to give a dimple at the water surface. The stirring was stopped after 23 hours and the mixture allowed to stand for 1-Hour. A wide bore glass tube, covered at one end with Nescofilm was submerged into the vessel, sealed end down, to a depth of approximately 5 cm from the bottom of the vessel. A length of Tygon tubing was inserted into the glass tube and pushed through the Nescofilm seal. The aqueous phase or WAF was removed by mid-depth siphoning (the first 75 to 100 mL discarded) to give the 1.0 mg/L loading rate WAF. Microscopic inspection of the WAF showed no micro-dispersions or undissolved test item to be present. A series of dilutions was made from the WAF to give further stock solutions of 0.32, 0.10, 0.032 and 0.010 mg/L loading rate WAF.
An aliquot (900 mL) of each of the loading rate WAFs was separately inoculated with algal suspension (5.3 mL) to give the required test concentrations of 0.010, 0.032, 0.10, 0.32 and 1.0 mg/L loading rate WAF.
The concentration and stability of the test item in the test preparations were verified by chemical analysis at 0 and 72 hours - Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- TEST ORGANISM
- Common name: green alga
- Strain: CCAP 278/4
- Source (laboratory, culture collection): Culture Collection of Algae and Protozoa (CCAP), SAMS Research Services Ltd, Scottish Marine Institute, Oban, Argyll, Scotland
- Method of cultivation: Master cultures were maintained in the laboratory by the periodic replenishment of culture medium
ACCLIMATION
- Culturing media and conditions (same as test or not): same
- Any deformed or abnormal cells observed: no - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Test temperature:
- 24 ±1 ºC
- pH:
- 7.3 - 7.4 at test start, 7.8 - 8.6 at the end of the observation period
- Nominal and measured concentrations:
- 0.010, 0.032, 0.10, 0.32 and 1.0 mg/L (nominal)
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 250 mL glass conical flasks containing 100 mL of test preparation
- Aeration: constantly shaken at approximately 150 rpm for 72 hours.
- Type (delete if not applicable): closed
- Initial cells density: 5.00E03 cells/mL
- Control end cells density: 1.63E06 cells/mL (mean)
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 6
GROWTH MEDIUM
- Standard medium used: yes
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water:
- Culture medium different from test medium: The culture medium used for both the range-finding and definitive tests was the same as that used to maintain the stock culture.
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: continuous illumination (intensity approximately 7000 lux) provided by warm white lighting (380 to 730 nm)
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: electronic particle counter
- Chlorophyll measurement: no
- Other:
Samples were taken at 19, 42 and 72 hours and the cell densities determined using a Coulter® Multisizer Particle Counter. Three determinations were made for each sample. The nominally inoculated cell concentration (5.00E03 cells/mL) was taken as the starting cell density. To determine the potential effect of the test item on the appearance of algal cells, a sample was removed from each test and control culture (replicates pooled) at the end of the test. The shape and size of the algal cells was inspected microscopically and any abnormalities recorded.
TEST CONCENTRATIONS
- Spacing factor for test concentrations: sqrt(10)
- Range finding study
- Test concentrations: first range-finding test: 10 and 100 mg/L, second range-finding test: 0.010, 0.10 and 1.0 mg/L
- Results used to determine the conditions for the definitive study:
The results showed no effect on growth at 0.010 mg/L loading rate WAF. However, growth was observed to be reduced at 0.10, 1.0, 10 and 100 mg/L loading rate WAF. Based on this information loading rates of 0.010, 0.032, 0.10, 0.32 and 1.0 mg/L were selected for the definitive test. - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
- Duration:
- 72 h
- Dose descriptor:
- EL10
- Effect conc.:
- 0.07 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- loading rate WAF
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EL50
- Effect conc.:
- 0.11 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- loading rate WAF
- Basis for effect:
- growth rate
- Remarks on result:
- other: 95% confidence limits
- Remarks:
- 0.10 to 0.13 mg/L
- Duration:
- 72 h
- Dose descriptor:
- EL10
- Effect conc.:
- 0.087 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- loading rate WAF
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- EL50
- Effect conc.:
- 0.092 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks:
- loading rate WAF
- Basis for effect:
- biomass
- Details on results:
- - Exponential growth in the control (for algal test): yes
- Observation of abnormalities (for algal test): no abnormalities detected in the control or test cultures at 0.010, 0.032 and 0.10 mg/L loading rate WAF; cell breakdown was observed in the test cultures at 0.32 and 1.0 mg/L loading rate WAF
- Effect concentrations exceeding solubility of substance in test medium:
Observations on the test media were carried out during the mixing and testing of the WAF.
At both the start and end of the mixing period and following a 1-Hour standing period, the 1.0 mg/L loading rate WAF was observed to have formed a clear colorless water column with a very small number of globules of test item dispersed throughout the water column. Microscopic examination of the WAF showed there to be no micro-dispersions of test item present.
At the start of the test all control and test cultures were observed to be clear colorless solutions. After the 72-Hour test period all control, 0.010 and 0.032 mg/L loading rate WAF test cultures were observed to be green dispersions. The 0.10 mg/L loading rate WAF test cultures were observed to be pale green dispersions whilst the 0.32 and 1.0 mg/L loading rate WAF test cultures were observed to be clear colorless solutions. - Results with reference substance (positive control):
- - Results with reference substance valid? yes
Exposure of Pseudokirchneriella subcapitata (CCAP 278/4) to the reference item gave the following results:
ErC50 (0 to 72 hour) : 1.6 mg/L; 95% confidence limits 1.4 to 1.8 mg/L
EyC50 (0 to 72 hour) : 0.77 mg/L; 95% confidence limits 0.68 to 0.87 mg/L
No Observed Effect Concentration based on growth rate: 0.25 mg/L
No Observed Effect Concentration based on yield: 0.25 mg/L
Lowest Observed Effect Concentration based on growth rate: 0.50 mg/L
Lowest Observed Effect Concentration based on yield: 0.50 mg/L
The results from the positive control with potassium dichromate were within the normal ranges for this reference item. - Reported statistics and error estimates:
- One way analysis of variance incorporating Bartlett's test for homogeneity of variance (Sokal and Rohlf, 1981) and Dunnett's multiple comparison procedure for comparing several treatments with a control (Dunnett, 1955) was carried out on the growth rate and yield data after 72 hours for the control, 0.010, 0.032 and 0.10 mg/L loading rates to determine any statistically significant differences between the test and control groups. All statistical analyses were performed using the SAS computer software package (SAS, 1999 - 2001). The 0.32 and 1.0 mg/L loading rates were not included in the analysis as visual inspection of the data showed a significant effect on growth.
- Validity criteria fulfilled:
- yes
- Conclusions:
- ErL10 (0 to 72 hour) : 0.070 mg/L loading rate WAF
ErL20 (0 to 72 hour) : 0.083 mg/L loading rate WAF
ErL50 (0 to 72 hour) : 0.11 mg/L loading rate WAF; 95% confidence limits
0.10 to 0.13 mg/L loading rate WAF
EyL10 (0 to 72 hour) : 0.087 mg/L loading rate WAF
EyL20 (0 to 72 hour) : 0.088 mg/L loading rate WAF
EyL50 (0 to 72 hour) : 0.092 mg/L loading rate WAF*
(* It was not possible to calculate 95% confidence limits for the EyL50 value as the data generated did not fit the models available for the calculation of confidence limits.) - Executive summary:
A study was performed to assess the effect of the test item on the growth of the green alga Pseudokirchneriella subcapitata. The method followed was designed to be compatible with the OECD Guidelines for Testing of Chemicals (2006) No 201, "Freshwater Alga and Cyanobacteria, Growth Inhibition Test" referenced as Method C.3 of Commission Regulation (EC) 761/2009.
Due to the complex multi-constituent nature of the test item, for the purposes of the study the test medium was prepared as a Water Accommodated Fraction (WAF) of the test item.
Following preliminary range-finding tests, Pseudokirchneriella subcapitata was exposed to WAFs of the test item over a range of nominal loading rates of 0.010, 0.032, 0.10, 0.32 and 1.0 mg/L (three replicate flasks per concentration) for 72 hours, under constant illumination and shaking at a temperature of 24 ±1 °C.
Samples of the algal populations were removed daily and cell concentrations determined for each control and treatment group, using a Coulter Multisizer Particle Counter.
Analysis of the test preparations at 0 hours showed measured test concentrations to range from 0.013 to 1.3 mg/L. A concentration dependent decline in measured test concentrations was observed at 72 hours in the range of 0.0086 to 1.3 mg/L (67% to 104% of the 0-hour measured test concentrations).
Given that the toxicity cannot be attributed to a single component or a mixture of components but to the test item as a whole, the results were based on nominal loading rates only.
Exposure of Pseudokirchneriella subcapitata to the test item gave the following results:
Response Variable
EL10
(mg/L Loading Rate WAF)EL50
(mg/L Loading Rate WAF95% Confidence Limits
(mg/L Loading Rate WAF)
No Observed Effect Loading Rate (NOEL) (mg/L)
Lowest Observed Effect Loading Rate (LOEL) (mg/L)
Growth Rate
0.070
0.11
0.10
-
0.13
0.032
0.10
Yield
0.087
0.092
*
0.032
0.10
* It was not possible to calculate 95% confidence limits for the EyL50 value as the data generated did not fit the models available for the calculation of confidence limits.
Reference
Validity criteria:
The following data show that the cell concentration of the control cultures increased by a factor of 327 after 72 hours. This increase was in line with the OECD Guideline that states the enhancement must be at least by a factor of 16 after 72 hours.
Nominal cell density of control at 0
hours : 5.00E03 cells per mL
Mean cell density of control at 72 hours : 1.63E06 cells
per mL
The mean coefficient of variation for section by section specific growth rate for the control cultures was 23% and hence satisfied the validation criterion given in the OECD Guideline which states the mean must not exceed 35%.
The coefficient of variation for average specific growth rate for the control cultures over the test period (0 to 72 hour) was 1% and hence satisfied the validation criterion given in the OECD Guideline which states that this must not exceed 7%.
Inhibition of Growth Rate and Yield in the Definitive Test
Nominal Loading Rate |
Growth Rate (cells/mL/hour) |
Yield (cells/mL) |
|||
0 to 72 Hour |
% Inhibition |
0 to 72 Hour |
% Inhibition |
||
Control |
R1 |
0.080 |
- |
1.59E+06 |
- |
R2 |
0.081 |
1.70E+06 |
|||
R3 |
0.082 |
1.77E+06 |
|||
R4 |
0.080 |
1.57E+06 |
|||
R5 |
0.081 |
1.69E+06 |
|||
R6 |
0.079 |
1.46E+06 |
|||
Mean |
0.081 |
1.63E+06 |
|||
SD |
0.001 |
1.13E+05 |
|||
0.010 |
R1 |
0.082 |
[1] |
1.86E+06 |
|
R2 |
0.084 |
[4] |
2.17E+06 |
|
|
R3 |
0.082 |
[1] |
1.83E+06 |
|
|
Mean |
0.083 |
[2] |
1.95E+06 |
[20] |
|
SD |
0.001 |
|
1.88E+05 |
|
|
0.032 |
R1 |
0.085 |
[5] |
2.32E+06 |
|
R2 |
0.082 |
[1] |
1.88E+06 |
|
|
R3 |
0.081 |
0 |
1.67E+06 |
|
|
Mean |
0.083 |
[2] |
1.96E+06 |
[20] |
|
SD |
0.002 |
|
3.34E+05 |
|
|
0.10 |
R1 |
0.057 |
30 |
2.88E+05 |
|
R2 |
0.051 |
37 |
1.95E+05 |
|
|
R3 |
0.047 |
42 |
1.47E+05 |
|
|
Mean |
0.052 |
36 |
2.10E+05 |
87 |
|
SD |
0.005 |
|
7.16E+04 |
|
|
0.32 |
R1 |
-0.002 |
102 |
-7.76E+02 |
|
R2 |
0.001 |
99 |
2.21E+02 |
|
|
R3 |
0.006 |
93 |
2.45E+03 |
|
|
Mean |
0.002 |
98 |
6.32E+02 |
100 |
|
SD |
0.004 |
|
1.65E+03 |
|
|
1.0 |
R1 |
-0.022 |
127 |
-4.00E+03 |
|
R2 |
-0.015 |
119 |
-3.27E+03 |
|
|
R3 |
-0.019 |
123 |
-3.71E+03 |
|
|
Mean |
-0.019 |
123 |
-3.66E+03 |
100 |
|
SD |
0.004 |
|
3.69E+02 |
|
R = Replicate
SD=Standarddeviation
- = Not applicable
[ ] = Increase in growth as compared to the control
Inhibition of Growth Rate and Yield in the Definitive Test
Nominal Loading Rate |
Growth Rate (cells/mL/hour) |
Yield (cells/mL) |
|||
0 to 72 Hour |
% Inhibition |
0 to 72 Hour |
% Inhibition* |
||
Control |
R1 |
0.080 |
- |
1.59E+06 |
- |
R2 |
0.081 |
1.70E+06 |
|||
R3 |
0.082 |
1.77E+06 |
|||
R4 |
0.080 |
1.57E+06 |
|||
R5 |
0.081 |
1.69E+06 |
|||
R6 |
0.079 |
1.46E+06 |
|||
Mean |
0.081 |
1.63E+06 |
|||
SD |
0.001 |
1.13E+05 |
|||
0.010 |
R1 |
0.082 |
[1] |
1.86E+06 |
|
R2 |
0.084 |
[4] |
2.17E+06 |
|
|
R3 |
0.082 |
[1] |
1.83E+06 |
|
|
Mean |
0.083 |
[2] |
1.95E+06 |
[20] |
|
SD |
0.001 |
|
1.88E+05 |
|
|
0.032 |
R1 |
0.085 |
[5] |
2.32E+06 |
|
R2 |
0.082 |
[1] |
1.88E+06 |
|
|
R3 |
0.081 |
0 |
1.67E+06 |
|
|
Mean |
0.083 |
[2] |
1.96E+06 |
[20] |
|
SD |
0.002 |
|
3.34E+05 |
|
|
0.10 |
R1 |
0.057 |
30 |
2.88E+05 |
|
R2 |
0.051 |
37 |
1.95E+05 |
|
|
R3 |
0.047 |
42 |
1.47E+05 |
|
|
Mean |
0.052 |
36 |
2.10E+05 |
87 |
|
SD |
0.005 |
|
7.16E+04 |
|
|
0.32 |
R1 |
-0.002 |
102 |
-7.76E+02 |
|
R2 |
0.001 |
99 |
2.21E+02 |
|
|
R3 |
0.006 |
93 |
2.45E+03 |
|
|
Mean |
0.002 |
98 |
6.32E+02 |
100 |
|
SD |
0.004 |
|
1.65E+03 |
|
|
1.0 |
R1 |
-0.022 |
127 |
-4.00E+03 |
|
R2 |
-0.015 |
119 |
-3.27E+03 |
|
|
R3 |
-0.019 |
123 |
-3.71E+03 |
|
|
Mean |
-0.019 |
123 |
-3.66E+03 |
100 |
|
SD |
0.004 |
|
3.69E+02 |
|
*In accordance with the OECD test guideline only the mean value for yield for each test concentration is calculated
R = Replicate
SD=Standarddeviation
- = Not applicable
[ ] = Increase in growth as compared to the control
Description of key information
72 h ErL10: 0.070 mg/L loading rate WAF
72 h ErL50: 0.11 mg/L loading rate WAF; 95% confidence limits: 0.10 to 0.13 mg/L loading rate WAF
(OECD TG 201; Pseudokirchneriella subcapitata; WAF)
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 0.11 mg/L
- EC10 or NOEC for freshwater algae:
- 0.07 mg/L
Additional information
A study was performed to assess the effect of the test item on the growth of the green alga Pseudokirchneriella subcapitata. The method followed was designed to be compatible with the OECD Guidelines for Testing of Chemicals (2006) No 201, "Freshwater Alga and Cyanobacteria, Growth Inhibition Test" referenced as Method C.3 of Commission Regulation (EC) 761/2009.
Due to the low aqueous solubility and complex nature of the test item for the purposes of the test the test item was prepared as a Water Accommodated Fraction (WAF).
Following preliminary range-finding tests, Pseudokirchneriella subcapitata was exposed to WAFs of the test item over a range of nominal loading rates of 0.010, 0.032, 0.10, 0.32 and 1.0 mg/L (three replicate flasks per concentration) for 72 hours, under constant illumination and shaking at a temperature of 24 ±1 °C.
Samples of the algal populations were removed daily and cell concentrations determined for each control and treatment group, using a Coulter Multisizer Particle Counter.
Analysis of the test preparations at 0 hours showed measured test concentrations to range from 0.013 to 1.3 mg/L. A concentration dependent decline in measured test concentrations was observed at 72 hours in the range of 0.0086 to 1.3 mg/L (67% to 104% of the 0-Hour measured test concentrations).
Given that the toxicity cannot be attributed to a single component or a mixture of components but to the test item as a whole, the results were based on nominal loading rates only.
Exposure of Pseudokirchneriella subcapitata to the test item gave the following results:
Response Variable |
EL50 |
95% Confidence Limits (mg/L Loading Rate WAF) |
EL10 |
No Observed Effect Loading Rate (NOEL) (mg/L) |
Lowest Observed Loading Rate (LOEL) (mg/L) |
||
Growth Rate |
0.11 |
0.10 |
- |
0.13 |
0.070 |
0.032 |
0.10 |
Yield |
0.092 |
|
* |
|
0.087 |
0.032 |
0.10 |
(*It was not possible to calculate 95% confidence limits for the EyL50value as the data generated did not fit the models available for the calculation of confidence limits.
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