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Diss Factsheets

Environmental fate & pathways

Biodegradation in water: screening tests

Administrative data

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2000-01-26 - 2000-02-24
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP and guideline compliant study.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2000
Report date:
2000

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to guideline
Guideline:
OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
Version / remarks:
1993
Deviations:
no
Qualifier:
according to guideline
Guideline:
EU Method C.4-D (Determination of the "Ready" Biodegradability - Manometric Respirometry Test)
Version / remarks:
29 Dec 1992
Deviations:
no
Qualifier:
according to guideline
Guideline:
ISO DIS 9408 (Ultimate Aerobic Biodegradability - Method by Determining the Oxygen Demand in a Closed Respirometer)
Version / remarks:
1999
Deviations:
no
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
2-methyl-1H,9H-pyrazolo[3,2-b]quinazolin-9-one
EC Number:
616-079-5
Cas Number:
74336-55-3
Molecular formula:
C11 H9 N3 O
IUPAC Name:
2-methyl-1H,9H-pyrazolo[3,2-b]quinazolin-9-one

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, non-adapted
Details on inoculum:
Municipal activated sludge from laboratory wastewater treatment plants fed with municipal sewage. Concentration of dry substance 30 mg/L.
Duration of test (contact time):
28 d
Initial test substance concentration
Initial conc.:
100 mg/L
Based on:
test mat.
Parameter followed for biodegradation estimation
Parameter followed for biodegradation estimation:
O2 consumption
Reference substance
Reference substance:
aniline

Results and discussion

% Degradation
Parameter:
% degradation (O2 consumption)
Value:
0 - <= 10
Sampling time:
28 d

BOD5 / COD results

Results with reference substance:
Degradation degree of the reference substance after 14 days (% BOD/ThOD) : 70-80
Degradation degree in the inhibition control after 14 days (% BOD/ThOD) : 40-50

Any other information on results incl. tables

Validity criteria:

Deviation of the degradation degree of the test substance in the plateau phase <20%

Degradation degree of the reference substance >60% after 14 days

Degradation degree in the inhibition control >25 % after 14 days

Oxygen demand in the blank control < 60 mg/I at the end of the test

pH values in the test assays ranged from pH 6 to 8.5 at the end of the test

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Interpretation of results:
other: poorly biodegradable
Conclusions:
The biodegradation of the test substance was below 20%.
Executive summary:

The biodegradability of the test substance was assessed in an aerobic test according to OECD guideline 301F and EU method C.4-D. (BASF, 2000) Activated sludge (non-adapted) was incubated at room temperature with the test substance at a concentration of 100 mg/L for 28 days. Eight replicates were investigated. The test substance was dispersed in the test media without any solubilizer and no pH adjustment had to be conducted. At the end of the test 0 – 10 % biodegradation was reached. The validity criteria were all met and the positive control substance aniline had shown the appropriate response with a degradation rate of 70 to 80 % after 14 days. Thus, under the test conditions the test substance was poorly biodegradable.