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EC number: 233-397-6 | CAS number: 10139-58-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Deviations:
- not specified
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.3 (Algal Inhibition test)
- Deviations:
- not specified
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- Analytical verification of all test concentrations at 0 and 72 hours.
Concentration of the stock solution also determined - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Stock solution 0.1 g/L prepared in deionised water.
- Controls: Blank control
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): None used - Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- TEST ORGANISM
- Strain: Desmodesmus subspicatus (CHODAT (86.81 SAG))
- Source (laboratory, culture collection): University of Gottingen, Germany.
- Age of inoculum (at test initiation): A preculture was taken from a stock culture 3 days prior to the test. Test cultures were innoculated at 20,000 cells/mL from the preculture - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Hardness:
- Not stated
- Test temperature:
- 23.4 - 23.5°C
- pH:
- pH 7.4 - 8.0 at start of test.
pH 7.9 - 9.6 at end of test - Nominal and measured concentrations:
- Nominal 0.02, 0.04, 0.08, 0.16, 0.32, 0.64, 1.2 and 2.4 mg/L
Measured concentrations at 0h: 0.03, 0.03, 0.06, 0.11, 0.22, 0.48, 0.9, 1.85 mg/L
Measured concentrations at 72h: <0.01 mg/L (calculated 0.01 mg/L), <0.01 mg/L (calculated 0.01 mg/L), <0.01 mg/L (calculated 0.02 mg/L), 0.06, 0.11, 0.25, 0.39, 0.45 mg/L
Geometrical mean measured concentrations: 0.019, 0.019, 0.037, 0.079, 0.158, 0.346, 0.593, 0.910 mg/L
It was not possible to calculate geometric mean measured concentrations for the three lowest test concentrations as measured concentrations after 72 h were <0.01 mg/L, therefore for these samples the 72 hour concentrations were calculated based on calculation of the mean value of % deviation from the 0 hour to 72 hour concentrations for the other test concentrations and using this to calculate the 72 concentrations for the other test concentrations. - Details on test conditions:
- Culture vessels: Sterile, aerated Erlenmeyer flasks
Initial cell density: 20,000 cells/ml
Growth medium: As described in EU method C2 and OECD guideline 201
Number of replicates per concentration: 3
Photoperiod: constant light
Light intensity:6000 - 1000 lux, white
Determination of cell concentration: spectrophotometer. - Reference substance (positive control):
- no
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.88 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 0.12 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 0.91 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC10
- Effect conc.:
- 0.26 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.037 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- other: growth rate and biomass
- Reported statistics and error estimates:
- Expected probits for each concentration calculated by probit transformation of the percentage inhibition rates over the regression line. NOEC determined on the basis of the area under the growth curve during the whole exposure time using the student t-test.
- Validity criteria fulfilled:
- yes
- Remarks:
- Control cell concentration increased by a factor of at least 16 within 3 days. Measured test concentrations after 72 h deviated by >20% therefore geometric mean measured concentrations used.
- Conclusions:
- The 72-hour EC50 and EC10 based on growth rate were >0.91 and 0.26 mg/L, respectively. The 72-hour EC50 and EC10 based on biomass were 0.88 and 0.12 mg/L, respectively. The 72-hour NOEC based on cell growth was 0.037 mg/L.
- Executive summary:
Enste-Diefenbach (2003) is a GLP compliant algal toxicity study following OECD guideline 201. Analytical verification of test concentrations was conducted and as concentrations at 72 hours were outside 20% of nominal, mean measured concentrations were used for reporting of results. Three replicates were included for each test concentration and the validity criteria given in the study report were met. There are some limitations with the reporting of the study, therefore it is assigned a Klimisch score of 2 (reliable with restrictions) and is used for assessment of this endpoint.
The 72-hour EC50 and EC10 based on growth rate were >0.91 and 0.26 mg/L, respectively. The 72-hour EC50 and EC10 based on biomass were 0.88 and 0.12 mg/L, respectively. The 72-hour NOEC based on cell growth was 0.037 mg/L.
Reference
Description of key information
The 72-hour EC50 and EC10 based on growth rate were >0.91 and 0.26 mg/L, respectively. The 72-hour EC50 and EC10 based on biomass were 0.88 and 0.12 mg/L, respectively. The 72-hour NOEC based on cell growth was 0.037 mg/L.
Key value for chemical safety assessment
Additional information
Enste-Diefenbach (2003) is a GLP compliant algal toxicity study following OECD guideline 201. Analytical verification of test concentrations was conducted and as concentrations at 72 hours were outside 20% of nominal, mean measured concentrations were used for reporting of results. Three replicates were included for each test concentration. The validity criteria given in the study report were met. There are some limitations with the reporting of the study, therefore it is assigned a Klimisch score of 2 (reliable with restrictions), however it is suitable for use for assessment of this endpoint.
The 72-hour EC50 and EC10 based on growth rate were >0.91 and 0.26 mg/L, respectively. The 72-hour EC50 and EC10 based on biomass were 0.88 and 0.12 mg/L, respectively. The 72-hour NOEC based on cell growth was 0.037 mg/L.
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