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EC number: 280-489-7 | CAS number: 83567-04-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Hydrolysis
Administrative data
Link to relevant study record(s)
- Endpoint:
- hydrolysis
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Study period:
- From January 21, 1999 to February 11, 1999
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study
- Remarks:
- RA study
- Reason / purpose for cross-reference:
- read-across: supporting information
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.7 (Degradation: Abiotic Degradation: Hydrolysis as a Function of pH)
- Deviations:
- no
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Remarks:
- HPLC and UV/Vis detector
- Details on sampling:
- Optimal concentration of the test substance: 98-176 mg/L
- Preparation of the samples:
5 to 9 mg test substance in a 50 mL volumetric flask with the relevant buffer (pH 4 or 7)
- Preparation of the standards:
3 to 8 mg standard test substance in a 50 mL volumetric flask with the relevant buffer (pH 4 or 7)
- Hydrolysis test procedure:
The flask containing the hydrolysis mixture was placed in a thermostatically controlled water bath at a selected temperature for a specified time. Thereafter it was cooled immediately. The pH was controlled. The HPLC analysis of the unhydrolysed test substance (main compound) was performed without any delay. - Buffers:
- - pH 4:
21.008 g citric acid monohydrat are dissolved in 200 mL sodium hydroxyde solution (1 mole/L) and the solution is adjusted to a volume of 1000 mLwith distilled water. 44 mL of hydrochloric acid (1 mole/L) are then added to 560 mL of this solution and adjusted to a volume of 1000 mL with distilled water. The solution is finally adjusted to pH 4.0 with NaOH or HCl.
- pH 7:
13.61g potassium dihydrogen phosphate are dissolved in 1000 mL distilled water. 29.63 mL of sodium hydroxyde solution (1 mole/L) are then added to 500 mL of this solution and adjusted to a volume of 1000 mL with distilled water. The solution is finally adjusted to pH 7.0 with NaOH or HCl.
- pH 9:
7.46 g potassium chloride and 6.184g boric acid are dissolved in 1000 mL distilled water. 500 mL of this solution are added to 21.3 mL sodium hydroxyde (1 mole/L) and adjusted to a volume of 1000 mL with distilled water. The solution is finally adjusted to pH 9.0 with NaOH or HCl. - Estimation method (if used):
- In order to estimate the half-life period of the test substance at room temperature for pH 4 and 7, an extrapolation according to the Arrhenius equation was performed for both pH-values.
- Details on test conditions:
- Pretest at 50°C and pH 4, 7 and 9 for 2.4 h or 5 d.
Test 1 at 50°C and pH 4 for 9 to 32 h.
Test 2 at 50°C and pH 7 for 2 to 5 h.
Test 3 at 55°C and pH4 for 0.5 to 24h.
Test 4 at 65°C and pH4 for 0.25 to 8h.
Test 5 at 35°C and pH7 for 1 to 24.25h. - Initial conc. measured:
- >= 100 - <= 180 mg/L
- Remarks:
- duration, pH and temperature: cfr "Details on test conditions"
- Preliminary study:
- The decomposition at pH 9 was higher than 50% after 2.4 h at 50°C. The test substance was thus considered hydrolytically instable at pH 9. According to the guideline, a half-life period of less than 1 day under environmental conditions (25°C) may be estimated. The pretests at pH 4 and 7 indicated that an examination of the hydrolysis kinetics was essential.
- Transformation products:
- not specified
- Key result
- pH:
- 9
- Temp.:
- 25 °C
- DT50:
- < 1 d
- Remarks on result:
- other: the test substance may be considered hydrotically instable at pH 9 (< 1 d at 25°C)
- Key result
- pH:
- 4
- Temp.:
- 25 °C
- DT50:
- 8.5 d
- Key result
- pH:
- 7
- Temp.:
- 25 °C
- DT50:
- 3 d
- Validity criteria fulfilled:
- yes
- Conclusions:
- Under the study conditions, the half-lives of the substance at 25°C and at pH 4 and 7 were determined to be 8.5 and 3.0 d, respectively and at pH 9 a half-life of less than 1 d was estimated.
- Executive summary:
A study was conducted to determine the hydrolysis as a function of pH of the read-across substance according to EU Method C.7, in compliance with GLP. Five to nine mg of test substance were weighed in a 50 mL volumetric flask and the relevant buffer (pH 4, 7 or 9) was added. The flask containing the hydrolysis mixture was placed in a thermostatically controlled water bath at selected temperatures (35, 50, 55 or 65°C) for a specified time (0.5 to 32 h). Thereafter it was cooled immediately. The pH was controlled. The HPLC analysis of the unhydrolysed test substance (main compound) was performed without any delay. Values for 25°C were determined by extrapolating the results of the experiment at higher temperatures. Under the study conditions, the half-lives of the substance at 25°C and at pH 4 and 7 were determined to be 8.5 and 3.0 d, respectively and at pH 9 a half-life of less than 1 d was estimated (Mühlberger, 1999).
Reference
Table 1. Results at pH 4
Temperature | t1/2 (by regression) | Experiment |
50°C | 25.61h | 1 |
55°C | 17.61h | 3 |
65°C | 8.54h | 4 |
25°C | 204.5h (=8.5d) | extrapolation |
Table 2. Results at pH 7
Temperature | t1/2 (by regression) | Experiment |
50°C | 3.93h | 2 |
35°C | 21.18h | 5 |
25°C | 71.5h (=3.0d) | extrapolation |
Description of key information
Key value for chemical safety assessment
- Half-life for hydrolysis:
- 3 d
- at the temperature of:
- 25 °C
Additional information
A study was conducted to determine the hydrolysis as a function of pH of the read-across substance according to EU Method C.7, in compliance with GLP. Five to nine mg of test substance were weighed in a 50 mL volumetric flask and the relevant buffer (pH 4, 7 or 9) was added. The flask containing the hydrolysis mixture was placed in a thermostatically controlled water bath at selected temperatures (35, 50, 55 or 65°C) for a specified time (0.5 to 32 h). Thereafter it was cooled immediately. The pH was controlled. The HPLC analysis of the unhydrolysed test substance (main compound) was performed without any delay. Values for 25°C were determined by extrapolating the results of the experiment at higher temperatures. Under the study conditions, the half-lives of the substance at 25°C and at pH 4 and 7 were determined to be 8.5 and 3.0 d, respectively and at pH 9 a half-life of less than 1 d was estimated (Mühlberger, 1999).
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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