(E)-methyl non-2-enoate

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

August - September 2017

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2017

Report date:

2017

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

(E)-methyl non-2-enoate

EC Number:

811-711-8

Cas Number:

14952-06-8

Molecular formula:

C10H18O2

IUPAC Name:

(E)-methyl non-2-enoate

Test material form:

liquid

Specific details on test material used for the study:

Batch: VE00470062

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

As the test item is a liquid with low water solubility (<100 mg/L) the slow-stirring method was applied for preparation of an equilibrated test item solution.

Test solutions

Vehicle:

no

Details on test solutions:

For determination of the appropriate test concentrations for the main test, a static range-finding test in a closed system was performed. The test concentrations were the undiluted equilibrated test medium with a loading rate of 100 mg/L and the dilutions 1:10 and 1:100.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

The study was performed with young daphnids of the species Daphnia magna Straus. A clone of this species (originally from the Daphnia Collection of the University of Basel/Switzerland in 2015) is successfully bred in IES Ltd Laboratories.

Study design

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Hardness:

250mg/L as CaCO3

Test temperature:

The test was performed in a temperature-controlled room with continuous monitoring of the room temperature. The water temperature was maintained at 21 °C.

Nominal and measured concentrations:

Based on the results of the stirring experiment and the range-finding test , the following concentrations of NEOFOLIONE were selected for the main test: the equilibrated test medium with a loading rate of 100 mg/L and the dilutions 1:2.2, 1:4.6, 1:10, 1:22 and 1:46. Additionally, a control (test water without test item) was tested in parallel.

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

2.4 mg/L

Nominal / measured:

meas. (TWA)

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

0.44 mg/L

Nominal / measured:

meas. (TWA)

Conc. based on:

test mat.

Basis for effect:

mobility

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Executive summary:

The 48h EC50 of Neofolione was determined to be 2.4 mg/L.