Tin bis(2-ethylhexanoate)

Long-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

long-term toxicity to aquatic invertebrates

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Justification for type of information:

see "read-across statement"

Reason / purpose for cross-reference:

read-across: supporting information

Reason / purpose for cross-reference:

read-across source

GLP compliance:

not specified

Duration:

3 wk

Dose descriptor:

EC50

Effect conc.:

1 500 µg/L

Nominal / measured:

nominal

Conc. based on:

element

Basis for effect:

reproduction

Duration:

3 wk

Dose descriptor:

LC50

Effect conc.:

42 mg/L

Nominal / measured:

nominal

Conc. based on:

element

Basis for effect:

mortality

Duration:

3 wk

Dose descriptor:

LOEC

Effect conc.:

350 µg/L

Nominal / measured:

nominal

Conc. based on:

element

Basis for effect:

reproduction

Details on results:

The 16% reproductive impairment concentration was taken as LOEC since it represented the minimal reproducible value below which no difference in the variability in reproduction could be detected in concentrations and controls.

Reported statistics and error estimates:

Results were said to be statistically evaluated with the method of Litchfield and Wilcoxon (1949) for establishing approximations of the 95% CI for survival. Reproductive effects were analysed in a similar manner excluding CIs. Reproductive effects are given in terms of reproductive impairment by percentage.

Validity criteria fulfilled:

not applicable

Remarks:

Details on mortality and mean number of live offspring of the parent animals in the controls not given

Conclusions:

Older study with some data gaps but considered useful for PNEC derivation in the EU risk assessment (ECB 2008)

Executive summary:

Study reporting on short term and long term toxicity of tin to Daphnia magna. Tests not done according to standard protocol, but of sufficient quality and considered useful for setting the PNEC freshwater. Study used for that purpose in the EU risk assessment process on tin (ECB 2008). Culture medium, test water conditions well described. Referred to the target substance the LOEC is 1.194 mg/L.

Reference 2

Endpoint:

long-term toxicity to aquatic invertebrates

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Justification for type of information:

Please refer to section 13 'Analogue Approach Rationale'

Reason / purpose for cross-reference:

read-across source

Qualifier:

according to guideline

Guideline:

OECD Guideline 211 (Daphnia magna Reproduction Test)

Duration:

21 d

Dose descriptor:

EC50

Effect conc.:

43.3 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

reproduction

Duration:

21 d

Dose descriptor:

LC50

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Remarks on result:

not determinable

Duration:

21 d

Dose descriptor:

NOEC

Effect conc.:

18.01 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

reproduction

Duration:

21 d

Dose descriptor:

LOEC

Effect conc.:

32.01 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

reproduction

Conclusions:

Half lethal concentration (LC50) of parent Daphnia magna exposed for 21 days is not be determined(LC50: >100 mg/L (95% confidence interval: not calculated). The 50% reproductive inhibitory concentration (EC50) of the exposure for 21 days is: 43.3 mg/L (95% confidence interval: 39.4-48.0 mg/L). The maximum no effect concentration (N0EC) and minimum no effect concentration (LOEC) of 21-day exposure on the cumulative number of litters per parent Daphnia magna is as follow: NOEC: 18.0 mg/L. LOEC: 32.0 mg/L

Executive summary:

Reproductive Inhibition Test of Sodium 2-Ethylhexanoate against Daphnia magna study is done under GLP condition. Animals were exposed total 21days in semi-static water flow system for concentrations: 10.0, 18.0, 32.0, 56.0, 100 mg/L. Half lethal concentration (LC₅₀) of parent Daphnia magna exposed for 21 days is not be determined(LC₅₀: >100 mg/L (95% confidence interval: not calculated). The 50% reproductive inhibitory concentration (EC₅₀) of the exposure for 21 days is: 43.3 mg/L (95% confidence interval: 39.4-48.0 mg/L). The maximum no effect concentration (N0EC) and minimum no effect concentration (LOEC) of 21-day exposure on the cumulative number of litters per parent Daphnia magna is as follow: NOEC: 18.0 mg/L. LOEC: 32.0 mg/L

Description of key information

Long-term toxicity to aquatic invertebrate studies with sodium bis(2-ethylhexanoate) and with SnCl2 are available:

Read-across with sodium bis(2-ethylhexanoate), OECD 211, Daphnia magna, semi-static, NOEC(21d) 18.0 mg/L.
Read-across with SnCl2, LOEC 350 µg/L Sn,  Referred to the target substance the LOEC is 1.194 mg/L.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Dose descriptor:

NOEC

Effect concentration:

1.194 mg/L

Additional information

Reproductive Inhibition Test of Sodium 2-Ethylhexanoate against Daphnia magna study is done under GLP condition. Animals were exposed total 21days in semi-static water flow system for concentrations: 10.0, 18.0, 32.0, 56.0, 100 mg/L. Half lethal concentration (LC₅₀) of parent Daphnia magna exposed for 21 days is not be determined(LC₅₀: >100 mg/L (95% confidence interval: not calculated). The 50% reproductive inhibitory concentration (EC₅₀) of the exposure for 21 days is: 43.3 mg/L (95% confidence interval: 39.4-48.0 mg/L). The maximum no effect concentration (N0EC) and minimum no effect concentration (LOEC) of 21-day exposure on the cumulative number of litters per parent Daphnia magna is as follow: NOEC: 18.0 mg/L. LOEC: 32.0 mg/L

 

Study reporting on short term and long term toxicity of tin to Daphnia magna. Tests not done according to standard protocol, but of sufficient quality and considered useful for setting the PNEC freshwater. Study used for that purpose in the EU risk assessment process on tin (ECB 2008). Culture medium, test water conditions well described. Referred to the target substance the LOEC is 1.194 mg/L.