Tetrabromophthalic anhydride

Water solubility

Link to relevant study record(s)

Reference

Endpoint:

water solubility

Type of information:

experimental study

Adequacy of study:

key study

Study period:

April 17, 2006 to May 9, 2006

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

EPA OPPTS 830.7840 (Water Solubility)

Version / remarks:

U.S. Environmental Protection Agency. 1996. Water Solubility: Column Elution Method; Shake Flask Method. Product Properties Test Guidelines. OPPTS 830.7840.

Deviations:

not specified

Qualifier:

according to guideline

Guideline:

OECD Guideline 105 (Water Solubility)

Version / remarks:

Organisation for Economic Cooperation and Development. 1995. Guideline for Testing of Chemicals, 105: Water Solubility.

Deviations:

not specified

GLP compliance:

yes

Type of method:

flask method

Specific details on test material used for the study:

No further details specified in the study report

Key result

Water solubility:

120 mg/L

Conc. based on:

test mat.

Incubation duration:

24 h

Temp.:

20 °C

pH:

7

Details on results:

Triplicate subsamples were removed from the appropriate solubility sample replicates after 24, 48 and 72 hours of shaking in a water bath maintained at 30 ± 1.0°C. Following a 24 hour equilibration period for each of the test vessels at 20 ± 0.5°C, triplicate aliquots were removed and analyzed for PHT4. The mean measured concentrations determined for the first, second and third intervals were 96.7 ± 0.440 mg/L, 124 ± 0.351 mg/L and 116 ± 3.91 mg/L, respectively. As the acceptance criterion for determining solubility is two consecutive intervals with results differing by no more than 15%, the definitive solubility of PHT4 was calculated as the mean concentration of the six values obtained from the second and third sampling intervals, or 120 ± 4.48 mg/L (CV=3.74, n=6).

Solubility of PHT4 in HPLC-grade Water

Sample Number (616C-109-)	Days at 30 °C	Concentration of PHT4		Mean Concentration by Interval (mg/L)
		Fortified (mg/L)	Measured (mg/L)
A1 A2 A3	1 1 1	500 500 500	97.1^1 96.2^1 96.8^1	96.7
B1 B2 B3	2 2 2	500 500 500	125 124 124	124
C1 C2 C3	3 3 3	500 500 500	116 115 116	116
Mean Solubility (mg/L) = Standard Deviation = Coefficient of Variation (%) = Number =			120 4.48 3.74 6
^1Results outside acceptance criteria of 15% and not included in the calculation of mean solubility. Results were generated using Excel 2000 in full precision mode. Manual calculations with rounded values may differ slightly.

 

Conclusions:

The solubility of PHT4 in HPLC-grade water was determined to be 120 ± 4.48 mg/L (CV=3.74, n=6).

Executive summary:

The solubility of PHT4 was determined in HPLC-grade water at 20 °C using the shake flask method. Samples were prepared for HPLC/UV analysis by diluting triplicate centrifuged aliquots from each test vessel, at each sampling interval, into the range of calibration standards for analysis. The solubility of PHT4 in HPLC-grade water was calculated as the mean PHT4 concentration determined in six samples over the course of two sampling intervals.

The solubility of PHT4 was determined to be 120 ± 4.48 mg/L (CV = 3.74, n=6).

Description of key information

The solubility of PHT4 was determined to be 120 ± 4.48 mg/L (CV = 3.74, n=6).

Key value for chemical safety assessment

Water solubility:

120 mg/L

at the temperature of:

20 °C

Additional information

The solubility of PHT4 was determined in HPLC-grade water at 20 °C using the shake flask method. Samples were prepared for HPLC/UV analysis by diluting triplicate centrifuged aliquots from each test vessel, at each sampling interval, into the range of calibration standards for analysis. The solubility of PHT4 in HPLC-grade water was calculated as the mean PHT4 concentration determined in six samples over the course of two sampling intervals.

The solubility of PHT4 was determined to be 120 ± 4.48 mg/L (CV = 3.74, n=6).