O-ethylhydroxylamine

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP guideline study with analytical monitoring

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1987

Report date:

1987

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

O-ethylhydroxylamine

EC Number:

402-030-3

EC Name:

O-ethylhydroxylamine

Cas Number:

624-86-2

Molecular formula:

C2 H7 N O

IUPAC Name:

O-ethylhydroxylamine

Details on test material:

- Name of test material (as cited in study report): Substance H109360 & Q125
- Physical state: colourless liquid
- Analytical purity: 50%
- Lot No.: EFI-100

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: A stock solution of the test substance was prepared ín dilution water. 1 L of each test solution was prepared by the addition of aliquots of stock solution to dilution water.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: water flea
- Source: obtained from continous laboratory cultures
- Age at study initiation: < 24 h
- Feeding during test: no

HOUSING
- Culture medium: a reconstituted water medium, identical to the test dilution water
- Temperature: 20 ± 2 °C
- Type and amount of food: defined diet of algae (Chlorella vulgaris) and yeast
- Photoperiod: 16 hours light : 8 hours dark

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Hardness:

162 mg/L as CaCO3

Test temperature:

19.6 - 20.8 °C

pH:

8.2 - 8.6

Dissolved oxygen:

9.0 -9.25 mg/L

Nominal and measured concentrations:

Nominal test concentrations: control, 1, 1.8, 3.2, 5.6, 10, 18 mg/L
Recovery rate of the test substance in the stock solution: 101%; test solutions were not analysed, since the estimated detection limit was 100 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Material, fill volume: Borosilicate glass beakers, 200 ml
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reconstituted water medium aerated for >2 h
- Alkalinity: 111 mg/L
- Conductivity: 482 µS/cm
- Intervals of water quality measurement: Dissolved oxygen the control was measured prior to the start of the test. Initial pH was determined of each test solution. At the end of the test the pH and dissolved oxygen concentration of two replicates of each control and treatment were measured. The temperature of water was measured in an additional at 0, 24 and 48 h.

OTHER TEST CONDITIONS
- Photoperiod: 16 h light :8 h darkness

EFFECT PARAMETERS MEASURED: mobility after 24 and 48 h

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

2.6 mg/L

Nominal / measured:

nominal

Conc. based on:

other: Testsolution with a water content of 50%

Basis for effect:

mobility

Remarks on result:

other: 95% CL: 2.3 - 2.9 mg/L

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

1.3 mg/L

Nominal / measured:

nominal

Conc. based on:

other: pure substance

Basis for effect:

mobility

Remarks on result:

other: 95% CL: 1.1 - 1.5 mg/L