Ethyl 3,5,5-trimethylhexanoate

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1983

Report date:

1983

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 401 (Acute Oral Toxicity)

Deviations:

no

GLP compliance:

no

Remarks:

study performed before implementation of GLP

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Ethyl 3,5,5-trimethylhexanoate

EC Number:

266-959-4

EC Name:

Ethyl 3,5,5-trimethylhexanoate

Cas Number:

67707-75-9

Molecular formula:

C11H22O2

IUPAC Name:

ethyl 3,5,5-trimethylhexanoate

Details on test material:

- Name of test material (as cited in study report): 3,5,5-Trimethyl-ethyl capronate
- Analytical purity: 100%

Test animals

Species:

mouse

Strain:

CF-1

Sex:

male

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: Winkelmann
- Age at study initiation: young adult
- Weight at study initiation: 22 g (mean)
- Fasting period before study: yes
- Housing: no data
- Diet (e.g. ad libitum): pelleted species specific diet, ad libitum
- Water (e.g. ad libitum): tap water, ad libitum
- Acclimation period: 5 d

ENVIRONMENTAL CONDITIONS
- Temperature (°C): approx. 22
- Humidity (%): approx. 50
- Air changes (per hr): no data
- Photoperiod (hrs dark / hrs light): 12/12

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

olive oil

Details on oral exposure:

VEHICLE
- Concentration in vehicle: 185 - 397 g/L

MAXIMUM DOSE VOLUME APPLIED: 20 cm³/kg for all dose levels

Doses:

3160, 3980, 5010, 5660, 6310, 7940 mg/kg bw

No. of animals per sex per dose:

10

Control animals:

no

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: no data
- Necropsy of survivors performed: no data

Results and discussion

Effect levels

Key result

Sex:

male

Dose descriptor:

LD50

Effect level:

5 280 mg/kg bw

Based on:

act. ingr.

95% CL:

>= 4 700 - <= 5 900

Mortality:

3160 mg/kg bw: 0/10 animals died
3980 mg/kg bw: 2/10 animals died
5010 mg/kg bw: 3/10 animals died
5660 mg/kg bw: 6/10 animals died
6310 mg/kg bw: 8/10 animals died
7940 mg/kg bw: 10/10 animals died

Clinical signs:

reduced general condition, increased breathing frequency, abnormal posture (face-down position), diarrhea

Body weight:

no data

Gross pathology:

no data

Applicant's summary and conclusion

Interpretation of results:

GHS criteria not met

Conclusions:

Based on the results obtained after a single oral administration to male mice, the oral LD50 of 3,5,5-Trimethyl-ethyl capronate was determined to be 5280 mg/kg bw.

Executive summary:

In an acute oral toxicity study similar to OECD guideline 401, groups of 10 fasted male CF-1 mice were given a single oral dose of 3,5,5-Trimethyl-ethyl capronateat doses of 3160, 3980, 5010, 5660, 6310, 7940 mg/kg bw and observed for 14 days. No necropsy was performed; data on body weight changes are not available.

The following mortality was observed: 0/10 at 3160 mg/kg bw, 2/10 at 3980 mg/kg bw, 3/10 at 5010 mg/kg bw, 6/10 at 5660 mg/kg bw, 8/10 at 6310 mg/kg bw and 10/10 at 7940 mg/kg bw. Deaths occurred between day 1 and 4.

The surviving animals showed reduced general condition, increased breathing frequency, abnormal posture (face-down position), diarrhea.

The oral LD50 was determined to be 5280 mg/kg bw (95% c.i. 4700 – 5900 mg/kg bw).