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EC number: 208-336-1 | CAS number: 522-75-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Skin irritation / corrosion
Administrative data
- Endpoint:
- skin irritation: in vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study
- Justification for type of information:
- data is from guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 006
- Report date:
- 2006
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: OECD 429(Skin Sensitization: Local Lymph Node Assay)
- Principles of method if other than guideline:
- A pre-test was performed to determine the highest non-irritant test chemical concentration for the main LLNA assay
- GLP compliance:
- no
Test material
- Reference substance name:
- 2-(3-oxobenzo[b]thien-2(3H)-ylidene)benzo[b]thiophene-3(2H)-one
- EC Number:
- 208-336-1
- EC Name:
- 2-(3-oxobenzo[b]thien-2(3H)-ylidene)benzo[b]thiophene-3(2H)-one
- Cas Number:
- 522-75-8
- Molecular formula:
- C16H8O2S2
- IUPAC Name:
- 2-(3-oxobenzo[b]thien-2(3H)-ylidene)benzo[b]thiophene-3(2H)-one
- Test material form:
- solid
- Details on test material:
- - Name of test material (as cited in study report): 3H,3'H-2,2'-bi-1-benzothiophene-3,3'-dione
- Molecular formula : C16H8O2S2
- Molecular weight: 296.369 g/mol
- Smiles notation : O=C1c2c(S\C1=C1/Sc3c(cccc3)C1=O)cccc2
- InChl : 1S/C16H8O2S2/c17-13-9-5-1-3-7-11(9)19-15(13)16-14(18)10-6-2-4-8-12(10)20-16/h1-8H/b16-15-
- Substance type:Organic
- Physical state:Solid
Constituent 1
- Specific details on test material used for the study:
- - Name of test material (as cited in study report): 3H,3'H-2,2'-bi-1-benzothiophene-3,3'-dione
- Common Name: Thioindigo
- Molecular formula: C16H8O2S2
- Molecular weight: 296.369 g/mol
- Smiles notation: O=C1c2c(S\C1=C1/Sc3c(cccc3)C1=O)cccc2
- InChl : 1S/C16H8O2S2/c17-13-9-5-1-3-7-11(9)19-15(13)16-14(18)10-6-2-4-8-12(10)20-16/h1-8H/b16-15-
- Substance type: Organic
- Physical state: Solid
- Purity: >95%(w/w)
- Batch No.: AAFE 094505
- Stability: 0.1 mg/ml 4h in DMSO, 50 mg/ml 72h in DMSO
- Storage: At room temperature
- Expiration Date: October 21, 2015
- Manufacturing Date: October 21, 2005
Test animals
- Species:
- mouse
- Strain:
- other: CBA/CaOlaHsd
- Details on test animals or test system and environmental conditions:
- Details on test animals and environmental conditions
Test Animals:
- Source: Harlan Netherlands
- Sex: Females
- Age: 6 - 12 weeks (beginning of acclimatization)
- Identification: Single caging. The animals will be distributed into the test groups at random and identified by cage number.
- Housing: single
- Cage Type: Makrolon Type I, with wire mesh top (EHRET GmbH, D-79302 Emmendingen) with granulated soft wood bedding (Harlan Winkelmann GmbH)
- Feed: pelleted standard diet, ad libitum (Harlan Winkelmann GmbH)
- Water: tap water, ad libitum, (Gemeindewerke, D-64380 Rossdorf)
- Acclimatisation: Under test conditions after health examination. Only animals without any visible signs of illness will be used for the study.
Environmental Conditions:
- Temperature: 22 + 3°C
- Relative humidity: 30-79%
- Artificial light: 6.00 a.m. - 6.00 p.m.
Test system
- Type of coverage:
- open
- Preparation of test site:
- not specified
- Vehicle:
- other: DMSO
- Controls:
- not specified
- Amount / concentration applied:
- 1.25, 2.5, 5 and 10% (w/v)
- Duration of treatment / exposure:
- single exposure
- Observation period:
- no data available
- Number of animals:
- 2 females
- Details on study design:
- The ears were observed for local irritation or systemic toxicity
Results and discussion
In vivo
Results
- Irritation parameter:
- overall irritation score
- Basis:
- mean
- Time point:
- other: no data available
- Reversibility:
- not specified
- Remarks on result:
- no indication of irritation
- Irritant / corrosive response data:
- The treatment of mice with 1.25, 2.5, 5 and 10% test item in DMSO did not show any signs of severe local irritation or systemic toxicity. Due to the discolouration by the test
item redness at the ears caused by irritation could not be observed.
Applicant's summary and conclusion
- Interpretation of results:
- other: not irritating
- Conclusions:
- The treatment of mice with 1.25, 2.5, 5 and 10% test item in DMSO did not show any signs of severe local irritation or systemic toxicity. Due to the discolouration by the test
item redness at the ears caused by irritation could not be observed.
Hence, the test chemical can be considered to be not irritating to the skin of mice. - Executive summary:
A pre-test was performed to determine the highest non-irritant test chemical concentration for the main LLNA assay.
1.25, 2.5, 5 and 10% (w/v) in DMSO were tested on one ear each of 2 female CBA/CaOlaHsd mice. The mice ears were observed for local irritation or systemic toxicity.
The treatment of mice with 1.25, 2.5, 5 and 10% test item in DMSO did not show any signs of severe local irritation or systemic toxicity. Due to the discolouration by the test
item redness at the ears caused by irritation could not be observed.
Hence, the test chemical can be considered to be not irritating to the skin of mice.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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