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EC number: 209-447-8 | CAS number: 579-75-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
- Justification for type of information:
- Data is from peer-reviewed journal
Data source
Reference
- Reference Type:
- publication
- Title:
- Antinociceptive, hypoglycemic and spasmolytic effects of Brickellia veronicifolia
- Author:
- Francisco Palacios-Espinosa, Myrna De´ ciga-Campos, Rachel Mataa
- Year:
- 2 008
- Bibliographic source:
- Journal of Ethnopharmacology 118 (2008) 448–454
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: as below
- Principles of method if other than guideline:
- Antinociceptive activity of 2-methoxybenzoic acid in mice was studied using Hot-plate test
- GLP compliance:
- not specified
- Test type:
- other: No data available
- Limit test:
- no
Test material
- Reference substance name:
- o-anisic acid
- EC Number:
- 209-447-8
- EC Name:
- o-anisic acid
- Cas Number:
- 579-75-9
- Molecular formula:
- C8H8O3
- IUPAC Name:
- 2-methoxybenzoic acid
- Test material form:
- solid: crystalline
- Details on test material:
- - Name of test material : 2-methoxybenzoic acid (2-MBA)
- Molecular formula : C8H8O3
- Molecular weight :152.1482 g/mole
- Smiles notation: COc1ccccc1C(=O)O
- InChl:1S/C8H8O3/c1-11-7-5-3-2-4-6(7)8(9)10/h2-5H,1H3,(H,9,10)
- Substance type: Organic
- Physical state: Solid
Constituent 1
- Specific details on test material used for the study:
- - Name of test material : 2-methoxybenzoic acid (2-MBA)
- Molecular formula : C8H8O3
- Molecular weight :152.1482 g/mole
- Smiles notation: COc1ccccc1C(=O)O
- InChl:1S/C8H8O3/c1-11-7-5-3-2-4-6(7)8(9)10/h2-5H,1H3,(H,9,10)
- Substance type: Organic
- Physical state: Solid
Test animals
- Species:
- mouse
- Strain:
- ICR
- Sex:
- male
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Centro UNAM-Harlan (Harlan Mexico, S.A. de C.V.).
- Age at study initiation: No data available
- Weight at study initiation: 20–25 g
- Fasting period before study: Yes, twelve hours before the experiments food was withheld, but animals had free access to drinking water.
- Housing: Animals were housed under standard laboratory conditions as per Mexican Official Norm for Animal Care and Handing (NOM-062-ZOO-1999) and in compliance with international rules on care and use of laboratory animals.
- Diet (e.g. ad libitum): Standard pellet diet, ad libitum
- Water (e.g. ad libitum): Water, ad libitum
- Acclimation period: No data available
ENVIRONMENTAL CONDITIONS
- Temperature (°C): No data available
- Humidity (%):No data available
- Air changes (per hr): No data available
- Photoperiod (hrs dark / hrs light): No data available
IN-LIFE DATES: From: To: No data available
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- other: saline solution with Tween® 80 (0.05%).
- Details on oral exposure:
- Details on exposure
VEHICLE
- Concentration in vehicle: 0, 1, 10, 31.6 and 100 mg/kg
- Amount of vehicle (if gavage): No data available
- Justification for choice of vehicle: No data available
- Lot/batch no. (if required): No data available
- Purity: No data available
MAXIMUM DOSE VOLUME APPLIED: No data available
DOSAGE PREPARATION (if unusual): 2-methoxybenzoic acid was suspended with Tween® 80 (0.05%) in saline solution (SS) and then administered orally.
CLASS METHOD (if applicable)
- Rationale for the selection of the starting dose: No data available - Doses:
- 0, 1, 10, 31.6 and 100 mg/kg bw
- No. of animals per sex per dose:
- Total: 30
0 mg/kg: 6 mice
1 mg/kg: 6 mice
10 mg/kg: 6 mice
31.6 mg/kg: 6 mice
100 mg/kg: 6 mice - Control animals:
- yes
- Details on study design:
- - Duration of observation period following administration: 14 days (or other?): No detailed data available
- Frequency of observations and weighing: Animals were observed before and 30, 60, 90, 120 and 150 min after treatments.
- Necropsy of survivors performed: No data available
- Other examinations performed: other: Antinociceptive activity were observed by using Hot-plate test. - Statistics:
- Data are expressed as the mean ±S.E.M. for the number (n=6) of animals in each group. In all cases the results of treated-animal (compounds, extracts and reference drugs) groups were compared with the appropriate control (vehicle) groups. Statistical analysis was performed with one-way ANOVA followed by Dunnett post hoc to determine the source of significant differences where appropriate; *P < 0.05 was considered statistically significant. Prisma Graph-Pad (version 4.0) software was used for statistics and plotting. Analysis of variance was also performed.
Results and discussion
- Preliminary study:
- No data avaialble
Effect levels
- Sex:
- male
- Dose descriptor:
- other: TDLo
- Effect level:
- 10 mg/kg bw
- Based on:
- test mat.
- Remarks on result:
- other: Significant increase in noxius stimulation
- Mortality:
- No data available
- Clinical signs:
- other: No data available
- Gross pathology:
- No data available
- Other findings:
- Antinociceptive activity:
A significant dose dependent increase was observed in pain response of treated mice when test by using hot plate test as compare to control.
Applicant's summary and conclusion
- Interpretation of results:
- Category 5 based on GHS criteria
- Conclusions:
- TDLo was considered to be 10 mg/kg when male ICR (Mus domesticus) mice were treated with 2-methoxybenzoic acid (2-MBA) orally.
- Executive summary:
Acute oral toxicity study was conducted to evaluate the antinociceptive effect of the test compound 2-methoxybenzoic acid (2-MBA) in Male ICR (Mus domesticus) mice in the concentration of 0, 1, 10, 31.6 and 100 mg/kg. Results show dose dependent increase in pain response of treated mice as compared to control. Therefore, TDLo was considered to be 10 mg/kg when male ICR (Mus domesticus) mice were exposed to 2-methoxybenzoic acid (2-MBA) orally.
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