Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 233-032-0 | CAS number: 10024-97-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to reproduction
Administrative data
- Endpoint:
- fertility, other
- Remarks:
- spermatogenesis
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
Data source
Reference
- Reference Type:
- publication
- Title:
- Testicular reaction to prolonged exposure to nitrous oxide
- Author:
- Kripke BJ, Kelman AD, Shah NK, Balogh K, Handler AH
- Year:
- 1 976
- Bibliographic source:
- Anesthesiology 44: 104–113 cited in 'Nitrous oxide [MAK Value Documentation, 1993]'
Materials and methods
Test guideline
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- Male rats were exposed to 200 000 ppm nitrous oxide for a maximum of 35 days and the testicular reaction was investigated.
- GLP compliance:
- no
- Limit test:
- no
Test material
- Reference substance name:
- Dinitrogen oxide
- EC Number:
- 233-032-0
- EC Name:
- Dinitrogen oxide
- Cas Number:
- 10024-97-2
- Molecular formula:
- N2O
- IUPAC Name:
- nitrogen oxide
- Test material form:
- gas
Constituent 1
Test animals
- Species:
- rat
- Strain:
- other: LEW/f Mai
- Sex:
- male
Administration / exposure
- Route of administration:
- inhalation: gas
- Type of inhalation exposure (if applicable):
- whole body
- Vehicle:
- air
- Analytical verification of doses or concentrations:
- yes
- Duration of treatment / exposure:
- 35 days at a maximum
- Frequency of treatment:
- 8 h/d
- Details on study schedule:
- Four to six rats were sacrificed by cardiac puncture and exsanguination after 1, 2, 3, 4, 5, 7, 10, 14, 21, 28, 32 and 35 days of exposure.
Doses / concentrations
- Dose / conc.:
- 200 000 ppm
- Remarks:
- (20%)
- No. of animals per sex per dose:
- In sum 135 animals were treated with nitrous oxide.
- Control animals:
- yes, concurrent vehicle
Examinations
- Parental animals: Observations and examinations:
- HORMONE MEASUREMENTS:
Blood, removed by cardiac puncture at the time of exsanguination, had its serum separated for measurement of testosterone level. - Sperm parameters (parental animals):
- Parameters examined in male animals:
testis weight, microscopic appearance of testis - Postmortem examinations (parental animals):
- SACRIFICE
- Male animals: All surviving animals
GROSS NECROPSY
- Gross necropsy consisted of external and internal examinations including the cervical, thoracic, and abdominal viscera.
HISTOPATHOLOGY / ORGAN WEIGHTS
Testis were prepared for microscopic examination and weighed, respectively.
Results and discussion
Results: P0 (first parental generation)
General toxicity (P0)
- Endocrine findings:
- no effects observed
- Description (incidence and severity):
- There was no significant difference in serum testosterone levels between the control and the experimental group.
- Organ weight findings including organ / body weight ratios:
- effects observed, treatment-related
- Histopathological findings: non-neoplastic:
- effects observed, treatment-related
- Description (incidence and severity):
- Injury of spermatogenic cells in the seminiferous tubules was observed. Cells, that had divided to become spermatocytes, spermatids, and spermatozoa by mitosis and meiosis, respectively, were subject to damage or destruction. A reduction in the number of spermatogenic cells with some disorganization of the normal architecture could also be observed. Cells in active division could be seen within seminiferous tubules that contained fewer spermatides and mature spermatozoa.
Effect levels (P0)
- Key result
- Dose descriptor:
- LOAEC
- Effect level:
- 200 000 ppm
- Based on:
- test mat.
- Sex:
- male
- Basis for effect level:
- reproductive function (sperm measures)
Target system / organ toxicity (P0)
- Key result
- Critical effects observed:
- yes
- Lowest effective dose / conc.:
- 200 000 ppm
- System:
- male reproductive system
- Organ:
- testes
- Treatment related:
- yes
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.