Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 203-687-7 | CAS number: 109-61-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
- Remarks:
- Only 4 test strains, S. typhimurium TA102/ E. coli WP2 uvrA are missing. However chloroformates are neither cross linking nor oxidizing agents.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 988
- Report date:
- 1988
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- no
- Principles of method if other than guideline:
- Only 4 test strains, S. typhimurium TA102/ E. coli WP2 uvrA are missing.
- GLP compliance:
- yes
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Propyl chloroformate
- EC Number:
- 203-687-7
- EC Name:
- Propyl chloroformate
- Cas Number:
- 109-61-5
- Molecular formula:
- C4H7ClO2
- IUPAC Name:
- propyl carbonochloridate
- Details on test material:
- - Name of test material (as cited in study report): chloroformic acid propylester
- Physical state: colorless liquid
- Purity: > 99%
- Batch No.: Versuch 29/1
- Expiration date of the lot/batch: not reported
- Stability under test conditions: lt is known, that chloroformic acid propylester hydrolysates very rapidly. Therefore, the stability of the test substance in the solvent ethanol and in aqua dest. was not determined analytically.
- Storage condition of test material: 4°C; solvent: ethanol
Constituent 1
Method
- Target gene:
- Salmonella typhimurium: (his-)
Species / strain
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Metabolic activation system:
- S-9 mix from Aroclor 1254 induced rat liver
- Test concentrations with justification for top dose:
- 0.0005 - 5.0 µl/plate (= approx. 5 to 5000 mg/plate)
- Vehicle / solvent:
- ethanol
Controls
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- Positive controls:
- yes
- Positive control substance:
- other: see free text
- Remarks:
- Dimethylcarbamoyl chloride was used as positive reference substance of the chemical class of the chloroformic acid ester
- Details on test system and experimental conditions:
- Preincubation assay
0.1 ml test solution, 0.1 ml bacterial suspension and 0.5 ml S-9 mix are incubated at 37°C for the duration of 20 minutes. Subsequently, 2 ml of soft agar is added and, after mixing, the samples are poured onto the Vogel-Bonner agar plates within approx. 30 seconds.
In each experiment 3 test plates per dose or per control used; after fter incubation at 37°C for 48 hours in the dark, the bacterial colonies (his revertants) are counted.
Positive control
with metabolic activation: 10 μg/plate 2-aminoanthracene for each strain;
without metabolic activation: 5 μg/plate N-methyl-N'-nitro-N-nitrosoguanidine for TA 100 and TA 1535, 10 μg/plate 4-nitro-o-phenylendiamine
for TA 98 and 100 μg 9-aminoacridine chloride monohydrate for TA 1537, all substances were dissolved in DMSO.
The titer was determined and in regularly measurements the strain characteristics were checked. Sterility control performed. - Evaluation criteria:
- Positive results
- doubling of the spontaneous mutation rate (control)
- dose-response relationship
- reproducibility of the result - Statistics:
- Mean and standard deviation calculated in result tables. No further data.
Results and discussion
Test resultsopen allclose all
- Species / strain:
- S. typhimurium TA 1535
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- A bacteriotoxic effect was observed at doses of about >0.002 - 0.005 µl/plate (without S-9 mix) and from about 0.3 µl - 0.5 µl/plate onward (with S-9 mix).
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 1537
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- A bacteriotoxic effect was observed at doses of about >0.002 - 0.005 µl/plate (without S-9 mix) and from about 0.3 µl - 0.5 µl/plate onward (with S-9 mix).
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 98
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- A bacteriotoxic effect was observed at doses of about >0.002 - 0.005 µl/plate (without S-9 mix) and from about 0.3 µl - 0.5 µl/plate onward (with S-9 mix).
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- A bacteriotoxic effect was observed at doses of about >0.002 - 0.005 µl/plate (without S-9 mix) and from about 0.3 µl - 0.5 µl/plate onward (with S-9 mix).
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- E. coli WP2
- Remarks:
- S. typhimurium TA102/ E. coli WP2 uvrA are missing.
- Metabolic activation:
- not applicable
- Genotoxicity:
- not determined
- Cytotoxicity / choice of top concentrations:
- not determined
- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'. Remarks: Salmonella typhimurium; TA98 TA100 TA1535 TA1537
Any other information on results incl. tables
Preincubation test | ||||||
Strain | Metabolic activation system | concentr. range (µl/plate) | Replicates | maximum revertant factor | dose dependency | Assessment |
TA 98 | no | .0005-.004 | 3 | 1.0 | no | negative |
yes | .05-.5 | 3 | 1.0 | no | negative | |
TA 100 | no | .0005-.004 | 3 | 0.7 | no | negative |
yes | .05-.5 | 3 | 0.9 | no | negative | |
TA 1535 | no | .02-5.0 | 3 | 0.0 | no | negative |
yes | .02-5.0 | 3 | 1.5 | no | negative | |
no | .0005-.02 | 3 | 0.9 | no | negative | |
yes | .05-.5 | 3 | 0.9 | no | negative | |
yes | .05-.5 | 3 | 0.9 | no | negative | |
TA 1537 | no | .0005-.004 | 3 | 0.7 | no | negative |
yes | .05-.5 | 3 | 0.9 | no | negative |
Applicant's summary and conclusion
- Conclusions:
- According to the results of the present study, the test substance Propyl chlorformate is not mutagenic in the Salmonella typhimurium reverse mutation assay under the experimental conditions chosen here.
- Executive summary:
The study is conducted according to the OECD Guideline 471and is reliable with acceptable restrictions. TA102/ E. coli WP2 uvrA arr missing. However chloroformates are neither cross linking nor oxidizing agents.
Propy chlorformate was tested in the preincubation assay with and without metabolic activation in S. typhimurium TA98, TA100, TA1535 and TA1537 at dose levels of 5 -5000 µg/plate. No increase in the number of revertants was detected in any strain with and without MA. Vehicle controls and positive controls were valid. Cytotoxicity was found depending on the strain and metabolic activation. No precipitation was reported.
According to the results of the present study, the test substance Propyl chlorformate is not mutagenic in the Salmonella typhimurium reverse mutation assay under the experimental conditions chosen here.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.