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EC number: 613-855-5 | CAS number: 65928-65-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_toxicological-information.png)
Endpoint summary
Administrative data
Description of key information
LD50 oral (rat): > 2000 mg/kg bw [Kurth 2007]
LD50 dermal (rat): > 2000 mg/kg bw [Kurth 2009a]
Key value for chemical safety assessment
Acute toxicity: via oral route
Link to relevant study records
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Aug to Nov 2005
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 423 (Acute Oral toxicity - Acute Toxic Class Method)
- Version / remarks:
- 17 December 2001
- Deviations:
- no
- GLP compliance:
- yes
- Test type:
- acute toxic class method
- Limit test:
- yes
- Species:
- rat
- Strain:
- Wistar
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Charles River, Sulzfeld, Germany
- Weight at study initiation: males: 104 - 109 g; females: 105 – 112 g
- Fasting period before study: 17.5 - 18.5 h
- Housing: individually in conventional cages
- Diet (e.g. ad libitum): pell. Ssniff ® R / M - H; ad libitum, 24 hours per day
- Water (e.g. ad libitum): filtrated tap water; ad libitum, 24 hours per day
- Acclimation period: ≥ 7 day
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 21-23
- Humidity (%): 55-60
- Photoperiod (hrs dark / hrs light): 12/12
- Route of administration:
- oral: gavage
- Vehicle:
- other: 2.0 mg carboxymethyl cellulose ad 1.0 mL Aqua dest.
- Details on oral exposure:
- VEHICLE
- Concentration in vehicle: 200 mg/mL
- Amount of vehicle (if gavage): 10 mL/kg
MAXIMUM DOSE VOLUME APPLIED: 10 mL/kg
CLASS METHOD
acute toxic class
- Rationale for the selection of the starting dose: Limit dose - Doses:
- 2000 mg/kg
- No. of animals per sex per dose:
- 3
- Control animals:
- no
- Details on study design:
- - Duration of observation period following administration: 21 days
- Frequency of observations and weighing: All alterations of the baseline condition of the animals were recorded. All animals were checked four times on administration day and once daily on the following days up to day 21 of the test. Body weight was determined at the start (day 1), on day 8 and day 15 and at the end of the study (day 21).
- Necropsy of survivors performed: yes - Key result
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- > 2 000 mg/kg bw
- Based on:
- test mat.
- Mortality:
- No mortality observed.
- Clinical signs:
- other: No clinical signs observed.
- Gross pathology:
- No adverse findings observed.
- Conclusions:
- A single oral administration of the test substance by gavage to male and female rats at the limit-dose 2000 mg/kg was tolerated without mortalities, clinical signs, effects on body weight gain and gross pathological findings. According to OECD TG 423 the oral LD50 of the test item is therefore > 2000 mg/kg body weight.
- Executive summary:
In an acute oral toxicity study according to OECD TG 423 (adopted 17 December 2001), groups (3/sex) of Wistar rats, were given a single oral dose of 5-Dehydrocyanomethylketon in 2.0 mg carboxymethyl cellulose ad 1.0 mL Aqua dest. at a dose of 2000 mg/kg bw. Animals were then observed for 21 days.
Oral LD50 Combined ≥ 2000 mg/kg bw
No mortality occurred during the conduction of this limit test.
Formulation is of low Toxicity based on the LD50 in males and females. The test item does not need to be classified with regard to acute oral toxicity.
There were no treatment related clinical signs, necropsy findings or changes in body weight.)
This acute oral study is classified as acceptable. It does satisfy the guideline requirement for an acute oral study OECD 423 in the rat.
Reference
No animal died in the course of the study. A single oral (gavage) application of 2000 mg/kg was tolerated without compound-related clinical findings. The body weight gain on days 8, 15 and 21 was within the normal range for rats of this age and strain, which are routinely used in the laboratory. Autopsy revealed no compound-related findings.
Endpoint conclusion
- Endpoint conclusion:
- no adverse effect observed
- Dose descriptor:
- discriminating dose
- Value:
- 2 000 mg/kg bw
Acute toxicity: via inhalation route
Endpoint conclusion
- Endpoint conclusion:
- no study available
Acute toxicity: via dermal route
Link to relevant study records
- Endpoint:
- acute toxicity: dermal
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- July to Nov 2006
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Reason / purpose for cross-reference:
- reference to same study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 402 (Acute Dermal Toxicity)
- Version / remarks:
- 24 February 1987
- Deviations:
- yes
- Remarks:
- - 3 instead of 5 animals/sex used
- GLP compliance:
- yes
- Test type:
- standard acute method
- Limit test:
- yes
- Species:
- rat
- Strain:
- Wistar
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Charles River, Sulzfeld, Germany
- Weight at study initiation: males: 283 – 290 g; females: 208 – 218 g
- Housing: individually in conventional cages
- Diet (e.g. ad libitum): pell. Ssniff® R / M - H, ad libitum, 24 hours per day
- Water (e.g. ad libitum): filtrated tap water, ad libitum, 24 hours per day
- Acclimation period: ≥ 7 days
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 21-23
- Humidity (%): 56-59
- Photoperiod (hrs dark / hrs light): 12/12
- Type of coverage:
- occlusive
- Vehicle:
- physiological saline
- Details on dermal exposure:
- TEST SITE
- Area of exposure: The backs of the animals, the paste was administered on a piece of gauze the size of 4 x 9 cm
- % coverage: approximately 10% of the body surface area of a rat
- Type of wrap if used: not reported; occlusive
REMOVAL OF TEST SUBSTANCE
- Washing: Substance residues were removed with warm water.
- Time after start of exposure: 24 h
TEST MATERIAL
- Amount(s) applied (volume or weight with unit): a paste consisting of the original compound [males: 566-580 mg ad 0.40 resp. 0.45 mL 0.9% (w/v) sodium chloride; females: 416.7-436.3 mg ad 0.30 mL 0.9% (w/v) sodium chloride]
- For solids, paste formed: yes
VEHICLE
- Amount(s) applied (volume or weight with unit): see above
- Concentration (if solution): see above
- Duration of exposure:
- 24 hours
- Doses:
- 2000 mg/kg bw
- No. of animals per sex per dose:
- 3
- Control animals:
- no
- Details on study design:
- - Duration of observation period following administration: 21days
- Frequency of observations and weighing: All alterations of the baseline condition of the animals were recorded. All animals were checked four times on administration day and once daily on the following days up to day 21 of the test. All alterations of the skin at the administration sites of all animals were recorded according to the scoring system recommended by the EU. The application sites were evaluated 1, 24, 48 and 72 h after removal of the patches. Body weight was determined at the start (day 1), on days 8 and 15 and at the end of the study (day 21).
- Necropsy of survivors performed: yes - Key result
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- > 2 000 mg/kg bw
- Based on:
- test mat.
- Remarks on result:
- other: test substance (technical quality including impurities)
- Mortality:
- No mortality was observed.
- Clinical signs:
- other: After administration of 2000 mg/kg of the test chemical focal to total hairless areas were found in one male animal (both fore- and hind limbs, inguinal region, abdomen) from day 15 onwards and in one female animal (both hind limbs, abdomen) on day 13 to
- Gross pathology:
- Autopsy revealed no compound-related findings, because the observed lesions (scab formation) in the skin are considered to be not substance related.
- Conclusions:
- A single dermal administration of the test substance (technical quality including impurities) to male and female rats at the limit-dose 2000 mg/kg was tolerated without mortalities and gross pathological findings. After administration of 2000 mg/kg the test item single animals showed hairless areas and thinning fur in the course of the study. These findings are suspected to be compound-related because they are known findings in rats after treatment with hormones. Additionally, a slight decrease in body weight was observed in some animals on day 8. This effect was reversible on day 15 of the test.
According to OECD TG 402 the dermal LD50 of the test item is therefore > 2000 mg/kg body weight. - Executive summary:
In an acute dermal toxicity study according to OECD TG 402 (adopted 17 December 1987), groups of young adult Wistar rats (3/sex) were dermally exposed to 5-Dehydrocyanomethylketon in physiological saline for 24 hours at a dose of 2000 mg/kg bw. Animals then were observed for 21 days.
Dermal LD50 Combined = 2000 mg/kg bw
No mortality occurred during the conduction of this limit test.
Dehydrocyanomethylketon is of low Toxicity based on the LD50 males and females.
After administration of 2000 mg/kg the test item single animals showed hairless areas and thinning fur in the course of the study. These findings are suspected to be compound-related because they are known findings in rats after treatment with hormones. Additionally, a slight decrease in body weight was observed in some animals on day 8. This effect was reversible on day 15 of the test.
No other effects were observed.
Reference
Endpoint conclusion
- Endpoint conclusion:
- no adverse effect observed
- Dose descriptor:
- discriminating dose
- Value:
- 2 000 mg/kg bw
Additional information
In an acute oral toxicity study according to OECD TG 423 (adopted 17 December 2001), groups (3/sex) of Wistar rats, were given a single oral dose of 5-Dehydrocyanomethylketon in 2.0 mg carboxymethyl cellulose ad 1.0 mL Aqua dest. at a dose of 2000 mg/kg bw. Animals were then observed for 21 days.
Oral LD50 Combined ≥ 2000 mg/kg bw
No mortality occurred during the conduction of this limit test.
Formulation is of low Toxicity based on the LD50 in males and females. The test item does not need to be classified with regard to acute oral toxicity.
There were no treatment related clinical signs, necropsy findings or changes in body weight.)
This acute oral study is classified as acceptable. It does satisfy the guideline requirement for an acute oral study OECD 423 in the rat.
In an acute dermal toxicity study according to OECD TG 402 (adopted 17 December 1987), groups of young adult Wistar rats (3/sex) were dermally exposed to 5-Dehydrocyanomethylketon in physiological saline for 24 hours at a dose of 2000 mg/kg bw. Animals then were observed for 21 days.
Dermal LD50 Combined = 2000 mg/kg bw
No mortality occurred during the conduction of this limit test.
Dehydrocyanomethylketon is of low Toxicity based on the LD50 males and females.
After administration of 2000 mg/kg the test item single animals showed hairless areas and thinning fur in the course of the study. These findings are suspected to be compound-related because they are known findings in rats after treatment with hormones. Additionally, a slight decrease in body weight was observed in some animals on day 8. This effect was reversible on day 15 of the test.
No other effects were observed.
Justification for classification or non-classification
Based on the study results a classification according to Regulation (EC) No. 1272/2008 (CLP) is not required.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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