BRUGGOLITE® FF6

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

October 1997- December 1997

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Version / remarks:

OECD 202

Deviations:

not specified

GLP compliance:

yes

Specific details on test material used for the study:

Details on properties of test surrogate or analogue material (migrated information):
PHYSICO-CHEMICAL PROPERTIES
- Melting point: no melting point/range (Decomposition at temperatures > 200 °C)
- Boiling point: no boiling point (decomposition before boiling)
- Vapour pressure: <1 * 10-12 hPa at 25°C (calculation)
- Water solubility: 125.76 g/L at 20°C
- log Pow: <-2
- pKa: = 7,00+/-0,04

Analytical monitoring:

no

Details on sampling:

analysis of the concentrations were not been performed.

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- method:the test article is a well soluble powder with strongly reducing properties. This would lead to total oxygen depletion in the test solutions. In arrangement with Dr. Peter (German environmental bureau, UBA, telephone call with Dr. Kuttler on 27 th October 1997) the test article was solved in the culturing medium (c= 10 g/l) and aerated with cleaned compressed air until complete oxidation. The time required for the oxidation amounted to 24 h at 20°C. Beginning at a redox potential of - 204 mV aeration changed the potential to + 85 mV after 24 h. The pH-value was regulated from 9,96 (after oxidation) to 7.8 +/- 0.2. The test solutions were produced by diluting this stock solution.

Test organisms (species):

other aquatic crustacea: Daphnia Magna, Clone 5

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia magna, Crustacea, Phyllopoda, Cladocera, Daphniidae
- Strain:clone 5
- Source: German Environmental Ministry
- Feeding during test: none
- water: see " details on test conditions "

Pre-treatment:
The holding of the animals is carried out in a non climated romm (22°C +/- 5°C, light/dark = 14h/10h; illumination: Grolux Aqua star) in 1L glass beakers. The animal are fed 3 times a day with alga (scenedesmus subspicatus) and once a day with washed activated sludge.

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Post exposure observation period:

no data available

Hardness:

total hardness: 2.5 mmol/L

Test temperature:

20°C +/- 2°C (Incubator, data given in the study report under "test conditions")
19.3 - 20.2 °C (data given in the study report under "results")

pH:

7.4 - 8.1

Dissolved oxygen:

> 65% over the test period in all vessels

Salinity:

stock solutions in demineralized water:
- synthetic sea salt (Wimex hw Meersalz, containing 7 macro nutrients, 60 trace elements, 8 vitamins and 3 amino acids): 0.333 g/L medium
- CaCl2 x 2H2O (117.6 g/L): 2.3 mL/Lmedium
- NaHCO3 (25.2 /L): 2.2 mL/L medium
- SeO2 (1.4 g/L): 0.1 mL/L medium

Nominal and measured concentrations:

concentrations: 10.000 mg/L, 5.000 mg/L, 2500 mg/L, 1250 mg/L, 625 mg/L, Control

Details on test conditions:

TEST SYSTEM
- Test vessel: 50 ml glass beakers
- Aeration: none
- No. of organisms per concentration: 20 animals/conc (4 x 5 in 20 mL, 50 mL glass beakers)
-Duration: 48 h

TEST MEDIUM / WATER PARAMETERS
Source/preparation of dilution water: the culturing medium (stock solutions in demineralized water):
-synthetic sea salt (Wimex hw Meersalz, containing 7 macro nutrients, 60 trace elements, 8 vitamins and 3 amino acids): 0.333 g/L medium
- CaCl2 x 2H2O (117.6 g/L): 2.3 mL/Lmedium
- NaHCO3 (25.2 /L): 2.2 mL/L medium
- SeO2 (1.4 g/L): 0.1 mL/L medium
This water is aerated with oil free compressed air until saturation with oxygen and the pH is adjusted to 7.8 +/- 0.2.

- Alkalinity: 0.8 mmol/L
- Ca/mg ratio: 4:1

OTHER TEST CONDITIONS
- Adjustment of pH: the pH is adjusted to 7.8 +/- 0.2.
- Illumination: 14h light, 10 h dark (Gro-Lux)

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
pH-value, dissolved oxygen value and temperature are measured at the beginning and at the end of the test.

TEST CONCENTRATIONS
- Test concentrations: 10000, 5000, 2500, 1250, 625 mg/L

Reference substance (positive control):

no

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

9 500 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

4 400 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

> 10 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

2 500 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

- Behavioural abnormalities: The surviving animals in 5 and 10 g/L are very weak by the end of the test, while the vitality of the animals in all other concentrations (till 2.5 g/L) resemble the control animals.
See also table 1.

Results with reference substance (positive control):

no reference substance used in the study

Reported statistics and error estimates:

none

table 1: Immobilisation rate, skinnings an no. of animals which are trapped at the surface after 48 h

	Control	625 [mg/L]	1250 [mg/L]	2500 [mg/L]	5000 [mg/L]	10000 [mg/L]
Immobilisation [%], 24 h	0	0	0	0	0	60
Immobilisation [%], 48 h	0	0	0	0	85	90
Skinnings [%], 48 h	100	100	100	100	75	50
animals trapped at thesurface [%], 48 h	0	0	0	0	5	0

Validity criteria fulfilled:

yes

Conclusions:

The test item is not acute toxic for daphnia.

Executive summary:

Observations: The surviving animals in 5 and 10 g/L are very weak by the end of the test, while the vitality of the animals in all other concentrations(till 2,5 g/L} resemble to the control animals.

EC₅₀(24h): 9500 mg/L

EC₅₀(48h): 4400 mg/L

Evaluation: The test item is not acute toxic for daphnia.

Description of key information

Not acute toxic to aquatic invertebrates (daphnia).
EC50(24h): 9500 mg/L
EC50(48h): 4400 mg/L
Evaluation: The test item is not acute toxic for daphnia.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

9 500 mg/L