Registration Dossier
Registration Dossier
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EC number: 939-682-8 | CAS number: -
Toxicity to soil microorganisms
Administrative data
Link to relevant study record(s)
Description of key information
EC50 (28 d) > 1000 mg a.i./kg dry soil (nominal, for the inhibition of nitrate formation, OECD 216)
Key value for chemical safety assessment
- Long-term EC10 or NOEC for soil microorganisms:
- 1 000 mg/kg soil dw
Additional information
There is one GLP study (Wildlife International, 2014) available in which the potential long-term adverse effects of the test item on the nitrogen transformation activity of soil microorganisms was investigated according to the OECD guideline 216.
The study was conducted with a sandy loam soil, which was adjusted to a moisture content of approximately 9 - 13%. After a 6 d soil acclimation period, the soil was amended with dried, ground alfalfa, dosed with five nominal test concentrations ranging from 62.5 - 1000 mg a.i./kg dry soil and incubated in the dark for 28 d in controlled, aerobic test conditions. Soil nitrate concentrations were measured by HPLC at the beginning and at the end of the test and the mean measured nitrate concentrations were compared to the negative control.
There was no inhibition of nitrate formation after 28 d and it was concluded that the EC50 value for inhibition after 28 d was greater than the highest tested concentration of 1000 mg a.i./kg dry soil.
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