Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 700-678-4 | CAS number: 1076-12-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 01 November to 05 December 2011
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-D (Determination of the "Ready" Biodegradability - Manometric Respirometry Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
Not applicable - Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge: Worlingworth sewage treatment works (Suffolk, UK),
- Laboratory culture: Yes
- Method of cultivation:At the time of collection, the sludge was sieved (1 mm2) then transported to the laboratory and left to stand for approximately 30 minutes to allow the sewage solids to settle. A portion of the supernatant was removed and the sludge aerated until required.
- Storage conditions: Aliquots (10 mL) of the sludge were filtered through dried and preweighed Whatman GF/C filters, which were then dried again at approximately 105°C for one hour
- Preparation of inoculum for exposure: The mixed liquor suspended solids (MLSS) content of the sludge was then determined and the volume required to give a solids level of 30 mg/L in test cultures was calculated. This was added to bottles three days before test initiation to allow a period of ageing. - Duration of test (contact time):
- 28 d
- Initial conc.:
- 50 other: mg O2/l
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Composition of medium: TCD in inoculated MSM (25 mgO2/500 mL)
- Test temperature: 22 ± 2ºC.
- pH: 7.5
- pH adjusted: no
- Aeration of dilution water: no
- Continuous darkness: no
TEST SYSTEM
- Culturing apparatus:
- Number of culture flasks/concentration:
Identity No. of vessels Contents
Blank – control 2 Inoculated MSM alone
Reference 1 Inoculated MSM + sodium benzoate (25 mgO2/500 mL)
Test 2 TCD in inoculated MSM (25 mgO2/500 mL)
Inhibition assay 1 TCD (25 mgO2/500 mL) + sodium benzoate (25 mgO2/500 mL) in inoculated MSM
- Method used to create aerobic conditions:
- Measuring equipment: Co-ordinated Environmental Services (CES) Ltd automated respirometer and associated software was used to monitor the cumulative amount of oxygen consumed by the mixtures.
- Test performed in closed vessels due to significant volatility of test substance: The CO2 absorber was replaced in each cell (to ensure the maximum adsorption capacity in the test) and the cultures were sealed and returned to the water bath to equilibrate. The cells were connected to the computer-controlled system and the test was initiated.
SAMPLING
- Sampling frequency: A record of the cumulative oxygen demand made by each cell was printed at, typically, hourly intervals.
- Sampling method: automated respirometer
CONTROL AND BLANK SYSTEM
- Inoculum blank: Yes
STATISTICAL METHODS: Not applicable - Reference substance:
- benzoic acid, sodium salt
- Preliminary study:
- None
- Test performance:
- None
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 3
- Sampling time:
- 1 d
- Details on results:
- Mean oxygen consumption in mixtures containing TCD was negligible during the test, at most, 3% of the ThOD on Day 1.
- Results with reference substance:
- In the presence of TCD the degradation of sodium benzoate achieved 60% after 5 days
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- under test conditions no biodegradation observed
- Conclusions:
- Substances are considered to be readily biodegradable in this type of test if oxygen consumption is equal to or greater than 60% of the theoretical value within ten days of the level achieving 10%. Therefore, TCD was not considered to be readily biodegradable under the conditions of this test.
Reference
Mean oxygen consumption in mixtures containing TCD was negligible during the test, at most, 3% of the ThOD on Day 1. Substances are considered to be readily biodegradable in this type of test if oxygen consumption is equal to or greater than 60% of the theoretical value within ten days of the level achieving 10%. Therefore, TCD was not considered to be readily biodegradable under the conditions of this test.
The results obtained for the rate of degradation of sodium benzoate (67% of its ThOD after 3 days) and for the cumulative amount of oxygen consumed by the control mixtures (26.36 and 28.40 mgO2/L) fulfilled the validity criteria for this test. In the presence of TCD the degradation of sodium benzoate achieved 60% after 5 days indicating that the test substance was not inhibitory to the microbial inoculum.
Description of key information
The substance is not readily biodegradable
Key value for chemical safety assessment
- Biodegradation in water:
- under test conditions no biodegradation observed
Additional information
In a test carried out in accordance with OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test), the test substance showed no measurable degradation over 28 days and therefore cannot be considered readily biodegradable
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.