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EC number: 700-316-5 | CAS number: 1155405-88-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- migrated information: read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Study period:
- 1999-02-01 to 1999-02-04
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: GLP guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 999
- Report date:
- 1999
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.3 (Algal Inhibition test)
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Tri (hexyl, octyl, decyl) citrate
- IUPAC Name:
- Tri (hexyl, octyl, decyl) citrate
- Reference substance name:
- -
- EC Number:
- 430-290-8
- EC Name:
- -
- IUPAC Name:
- 430-290-8
- Reference substance name:
- 2-Hydroxypropane-1,2,3-tricarboxylic acid, tri (hexyl, octyl, decyl) ester
- IUPAC Name:
- 2-Hydroxypropane-1,2,3-tricarboxylic acid, tri (hexyl, octyl, decyl) ester
- Details on test material:
- - Name of test material (as cited in study report): Tri (hexyl, octyl, decyl) citrate
- Substance type: pure active substance
- Physical state: liquid
- Stability under test conditions: in water for at least 2 hours
- Storage condition of test material: in original container, at room temperature, in the dark
Constituent 1
Constituent 2
Constituent 3
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
PHYSICO-CHEMICAL PROPERTIES
- Melting point: -29°C (freezing point: -35.5°C)
- Boiling point: test substance showed decomposition above 260°C
- Vapour pressure: 8.5 x 10E-3 p at 25°C
- Water solubility (under test conditions): < 1 mg/L
- Solubility in organic solvents: soluble
- log Pow: > 6
- pKa: not applicable
- Base or acid catalysis of test material: no data
- UV absorption: no data
- Stability of test material at room temperature: stable
- pH dependance on stability: no data
OTHER PROPERTIES (if relevant for this endpoint)
- Adsorption characteristics: no data
Sampling and analysis
- Analytical monitoring:
- no
- Details on sampling:
- In a pre-experiment (without GLP) the dissolved organic carbon content (DOC) of the test substance preparation (prepared as in test) was determined. In the sample the same amount of DOC was determined as in the blank control. Thus, no dissolved test substance could be verified in test water.
Therefore, no analytical dose verification in the test media of the algal growth inhibition test could be done
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: A supersaturated stock suspension of the test substance with a nominal concentration of 100 mg/L was prepared by weighing 50 mg of the test substance into 500 mL test water, following ultrasonic treatment for 10 minutes and intense stirring. The supersaturated stock suspension was stirred on a magnetic stirrer at room temperauter in the dark over 3 days . Thereafter the suspension was filtered through a cellulose-nitrate filter with a defined pore size of 0.2 µm just before the start of the test.
- Controls: test water
- Evidence of undissolved material (e.g. precipitate, surface film, etc): no, suspension was filtered
Test organisms
- Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- TEST ORGANISM
- Common name. green algae
- Strain: Scenedesmus subspicatus CHODAT Strain No. 86.81 SAG
- Source (laboratory, culture collection): Sammlung von Algenkulturen, Pflanzenphysiologisches Institut, University of Göttingen, Germany
- Age of inoculum (at test initiation): 3 days old pre-culture (exponentially growing)
- Method of cultivation: in the testing laboratory under standardised conditions according to the test guidelines
ACCLIMATION
- Acclimation period: 3 days
- Culturing media and conditions (same as test or not): same as test
- Any deformed or abnormal cells observed: not mentioned
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 72 h
Test conditions
- Hardness:
- 0.24 mmol/L as CaCO3 (calculated)
- Test temperature:
- 23.0 - 23.3 °C
- pH:
- start of test: 7.6 - 7.8
end of test: 9.6 - 10.0 (The increase of the pH was obviously caused by the CO2-consumption of the algae due to their rapid growth respectively their high densities) - Dissolved oxygen:
- not mentioned
- Salinity:
- In deionized water (conductivity < 5 µS/cm) analytical grade salts were added to following nominal concentrations:
Macro-nutrients: 50.0 mg/L NaHCO3; 18.0 mg/L CaCl2 x 2 H2O; 15.0 mg/L NH4Cl; 15.0 mg/L MgSO4 x 7 H2O; 12.0 mg/L MgCl2 x 6 H2O; 1.6 mg/L KH2PO4
Trace elements: 100 µg/L Na2EDTA x 2 H2O; 80 µg/L FeCl3 x 5 H2O; 415 µg/L MnCl2 x 4 H2O; 185 µg/L H3BO3; 7 µg/L Na2MoO4 x 2 H2O; 3 µg/L ZnCl2; 1.5 µg/L CoCl2 x 6 H2O; 0.01 µg/L CuCl2 x 2 H2O - Nominal and measured concentrations:
- WAF, no analytical determination of concentration
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 50 mL Erlenmeyer flasks with 50 mL test medium covered with glass dishes, continuously stirred by magnetic stirrers, incubated in a water bath
- Aeration: not aerated
- Initial cells density: 10000 algal cells/mL
- Control end cells density: 92.23 x 10E4 cells/mL
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 6
GROWTH MEDIUM
- Standard medium used: yes
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: deionized water (conductivity < 5 µS/cm)
- Ca/mg ratio: not mentioned
- Culture medium different from test medium: no
- Intervals of water quality measurement: not mentioned
OTHER TEST CONDITIONS
- Sterile test conditions: yes
- Adjustment of pH: no
- Photoperiod: continuous illumination
- Light intensity and quality: 7910 Lux (mean value, range: 7830 to 8000 Lux), achieved by fluorescent tubes (Universl Weiss L36W/25-1), installed above the water bath
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: spectrophotometrical measurement of cell density after 26, 48 and 72 hours (The cell density in one control was counted by microscope after 72 hours. Based on the counted cell densities and based on the determined absorption of the control and five dilutions of the control a linear regression was performed for the calculation of the cell densities in all other samples measured spectrophotometrical during the test.)
- Chlorophyll measurement: no
- Other:
For the determintation of an influence of the test substance on the algal cells, from the filtate a sample was taken after 72 hours test duration. The shape of the treated algal cells compared to the control was microscopically examined.
The behaviour of the test substance in the filtrate was determined daily.
Measurement of pH-values in samples of the test media and the controls at the start and at the end of the test.
The test media temperature was measured daily in an Erlenmeyer flask filled with water and incubated under the same conditions as the test flasks. - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate, tested at least twice a year
Results and discussion
- Details on results:
- - Exponential growth in the control: yes
- Observation of abnormalities (for algal test): no abnormalities observed
- Any stimulation of growth found in any treatment: no
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: no remarkable observation made concerning the appearance of the test substance in the filtrate
- Effect concentrations exceeding solubility of substance in test medium: no concentrations exceeding solubility tested - Results with reference substance (positive control):
- not mentioned
- Reported statistics and error estimates:
- The LOEC, the EbC50 and ErC50 and the corresponding EC10 and their 95%-confidence limits could not be calculated due to the absence of toxicity of the test substance up to the water solubility limit of the test substance.
Any other information on results incl. tables
Table: Algal cell densities during the test period of 72 hours
Treatment | Density of algal cells (x 10E4/mL)* after | ||
24 h | 48 h | 72 h | |
control | 8.10 ± 0.41 | 22.20 ± 1.98 | 92.23. ± 0.79 |
test substance filtrate | 11.57 ± 0.49 | 20.07 ± 0.99 | 91.47 ± 0.49 |
* mean values ± standard deviation
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Remarks:
- cell density in control cultures increased by a factor of at least 16 within 72 hours
- Conclusions:
- Due to the low water solubility of the test substance a filtrate of a supersaturated stock suspension of nominal 100 mg/L was tested. Thus, no concentration above the solubility limit of the test substance in the used test water were tested. Also due to the low water solubility no analytical verification could be done in the present test. Therefore, the biological results could not be related to a specific concentration of the test substance but only to the water solubility limit in the test medium.
The 72-hour NOEC was determined to be at least up to the solubility limit of the test substance. The NOEC might even be higher than this concentration, but concentrations in excess of the solubility limit had not been tested. The 72-hour LOEC and the 72-hour EC50 were clearly higher than the solubility limit. - Executive summary:
Due to the low water solubility of the test substance a filtrate of a supersaturated stock suspension of nominal 100 mg/L was tested. Thus, no concentration above the solubility limit of the test substance in the used test water were tested. Also due to the low water solubility no analytical verification could be done in the present test. Therefore, the biological results could not be related to a specific concentration of the test substance but only to the water solubility limit in the test medium.
The 72-hour NOEC was determined to be at least up to the solubility limit of the test substance. The NOEC might even be higher than this concentration, but concentrations in excess of the solubility limit had not been tested. The 72-hour LOEC and the 72-hour EC50 were clearly higher than the solubility limit.
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