complex reaction mass of Chinese gum rosin post reacted with acrylic acid

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Guideline study and GLP

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1999

Report date:

1999

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Alga, Growth Inhibition Test)

Test guideline 2

Qualifier:

according to guideline

Guideline:

other: Method C3, 92/69/EEC

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Hydrogenated reaction products of rosin with acrylic acid

Cas Number:

144413-22-9

IUPAC Name:

Hydrogenated reaction products of rosin with acrylic acid

Test solutions

Details on test solutions:

Identity and concentration of auxiliary solvent for dispersal: Peliminary solutions were prepared in dimethylformamide at a
concentration of 100mg/10ml solvent. An aliquot of this
solution was then dispersed in culture medium to give the
1mg/l test concentration.

Test organisms

Test organisms (species):

Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

72 h

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

> 0.49 mg/L

Basis for effect:

growth rate

Effect concentrations 2

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

> 0.49 mg/L

Basis for effect:

growth rate

Details on results:

%Concentration loss over test: 81 ... 76

Any other information on results incl. tables

For the purpose of the definitive study the test material was prepared using a preliminary solution in dimethylformamide. An amount of test material (100 mg) was dissolved in dimethylformamide and the volume adjusted to 10 ml to give a 100 mg/10 ml solvent stock solution. An aliquot (500 µl) of this solvent stock solution was dispersed in algal suspension (5 Litres) to give the test concentration of 1.0 mg/l.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

Exposure of Scenedesmus subspicatus to the test material gave EC50 values of greater than 1.0 mg/I and correspondingly the No Observed Effect Concentration was equal to 1.0 mg/I. The test concentration of 1.0 mg/I was the highest attainable test concentration that could be prepared due to the limited solubility of the test material in water and auxiliary solvent and having due regard to the amount of auxiliary solvent permitted in the test under the OECD Guidelines.

Executive summary:

The effect of the test material on the growth of Scenedesmus subspicatus has been investigated and gave EC50 values of greater than 1.0 mg/I. Correspondingly the No Observed Effect Concentration was equal to 1.0 mg/l. Based on the time-weighted mean measured test concentration the EC50 values were calculated to be greater than 0.49 mg/l. Correspondingly the No Observed Effect Concentration was equal to 0.49 mg/l.