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EC number: 203-266-8 | CAS number: 105-06-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- data from handbook or collection of data
- Justification for type of information:
- Data is from peer reviewed publication
Data source
Reference
- Reference Type:
- publication
- Title:
- Gene mutation toxicity study of the the test chemical
- Author:
- McMohan et al
- Year:
- 1 979
- Bibliographic source:
- Cancer Research
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Version / remarks:
- Modified method
- Principles of method if other than guideline:
- Gene mutation toxicity study was performed to determine the mutagenic nature of the test chemical
- GLP compliance:
- not specified
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- 1-bromo-4-methoxybenzene
- Cas Number:
- 104-92-7
- Molecular formula:
- C7H7BrO
- IUPAC Name:
- 1-bromo-4-methoxybenzene
- Details on test material:
- - Name of test material: p-bromoanisole- Molecular formula: C7H7BrO- Molecular weight: 187.035 g/mol- Substance type: Inorganic- Physical state: No data- Purity: No data- Impurities (identity and concentrations): No data
Constituent 1
Method
- Target gene:
- Histidine for Salmonella typhimurium strains and tryptophan for E. coli strains
Species / strainopen allclose all
- Species / strain / cell type:
- S. typhimurium, other: G46, TA1535, TA100, C3076, TA1537, D3052, TA1538, TA98
- Details on mammalian cell type (if applicable):
- Not applicable
- Additional strain / cell type characteristics:
- other:
- Species / strain / cell type:
- E. coli, other: WP2 and WP2 uvrA-
- Details on mammalian cell type (if applicable):
- Not applicable
- Additional strain / cell type characteristics:
- other: WP2 (E. coli tryptophan) and WP2 uvrA (E. coli tryptophan with uvrA deletion).
- Cytokinesis block (if used):
- No data
- Metabolic activation:
- with and without
- Metabolic activation system:
- Liver enzymes were prepared from adult male Fischer rats predosed with Aroclor 1254
- Test concentrations with justification for top dose:
- 0.1 – 1000 µg/mL
- Vehicle / solvent:
- - Vehicle(s)/solvent(s) used: DMSO, water or dimethoxyethane- Justification for choice of solvent/vehicle: Solubility
Controls
- Untreated negative controls:
- not specified
- Negative solvent / vehicle controls:
- yes
- Remarks:
- Details not specified
- True negative controls:
- not specified
- Positive controls:
- yes
- Positive control substance:
- 2-acetylaminofluorene
- other: Streptozotocin (without microsomal activation)
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: in agar (gradient plate method)DURATION- Preincubation period: No data- Exposure duration: 48 hrs- Expression time (cells in growth medium): 48 hrs- Selection time (if incubation with a selection agent): No data- Fixation time (start of exposure up to fixation or harvest of cells): No dataSELECTION AGENT (mutation assays): No dataSPINDLE INHIBITOR (cytogenetic assays): No dataSTAIN (for cytogenetic assays): No dataNUMBER OF REPLICATIONS: Duplicate sets of plates were prepared: one set in which the test compound was in the bottom wedge and had to diffuse upward and one in which it was in the upper wedge and had to diffuse downward.NUMBER OF CELLS EVALUATED: No dataDETERMINATION OF CYTOTOXICITY- Method: mitotic index; cloning efficiency; relative total growth; other: No dataOTHER EXAMINATIONS:- Determination of polyploidy: No data- Determination of endoreplication: No data- Other: No dataOTHER: No data
- Rationale for test conditions:
- No data
- Evaluation criteria:
- Bacterial growth was observed along the streak line and the concentration range over which chemically induced mutant colonies are present is recorded.
- Statistics:
- No data
Results and discussion
Test resultsopen allclose all
- Species / strain:
- S. typhimurium, other: G46, TA1535, TA100, C3076, TA1537, D3052, TA1538, TA98
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not specified
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not specified
- Positive controls validity:
- valid
- Species / strain:
- E. coli, other: WP2 and WP2 uvrA-
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not specified
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not specified
- Positive controls validity:
- valid
- Additional information on results:
- No data
- Remarks on result:
- other: No mutagenic potential
Applicant's summary and conclusion
- Conclusions:
- The test chemical did not induce gene mutation in Salmonella typhimurium G46, TA1535, TA100, C3076, TA1537, D3052, TA1538, TA98 and E. coli WP2 and WP2 uvrA- in the presence and absence of Liver enzymes activation system and hence the chemical is not likely to classify as a gene mutant in vitro.
- Executive summary:
Gene mutation toxicity study was performed to determine the mutagenic nature of the test chemical. The study was performed using Salmonella typhimurium G46, TA1535, TA100, C3076, TA1537, D3052, TA1538, TA98 and E. coli WP2 and WP2 uvrA- with and without Liver enzymes activation system.
Ten ml of minimal agar medium (not containing test compound) was poured into a square Petri dish (9 x 9 cm) which is tilted at a slight angle. The agar was then allowed to solidify into a wedge-shaped layer by standing at room temperature. Meanwhile, a 1000-µg/ml mixture of test compound in agar was prepared by adding 10 ml of minimal agar to 0.1 ml of a 100-mg/mI solution of test compound in dimethyl sulfoxide. When appropriate, water or dimethoxyethane was used instead of dimethyl sulfoxide. The cooled agar plates were then placed on a level surface, and an overlay of the 10 ml of agar containing the test compound was poured onto the plate to form a reversed wedge of agar on top of the first wedge. A concentration gradient of compound was produced by allowing the compound in the upper wedge to diffuse into the lower layer for 2 hr at room temperature. The concentration range in this plate is approximately 100 to 1000µg/ml. Three additional plates with concentration ranges of 10 to 100µg/ml, 1 to 10µg/ml, and 0.1 to 1µg/ml were prepared.
A streaking device consisting of 10 sterile 50-µL pipets was dipped into suspensions of the 10 test strains and allowed to fill by capillary action. The pipets were then touched to the upper edge of the gradient and drawn across the plate. The study was performed in the presence and absence of liver enzyle activating system and the plates were incubated for 48 hrs at 37°C.
The test chemical did not induce gene mutation in Salmonella typhimurium G46, TA1535, TA100, C3076, TA1537, D3052, TA1538, TA98 and E. coli WP2 and WP2 uvrA- in the presence and absence of Liver enzymes activation system and hence the chemical is not likely to classify as a gene mutant in vitro.
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