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EC number: 222-813-1 | CAS number: 3618-72-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- other: data sharing dispute
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 986
- Report date:
- 1986
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 401 (Acute Oral Toxicity)
- Deviations:
- not specified
- GLP compliance:
- yes
- Limit test:
- yes
Test material
- Reference substance name:
- 2,2'-[[5-acetamido-4-[(2-bromo-4,6-dinitrophenyl)azo]-2-methoxyphenyl]imino]diethyl diacetate
- EC Number:
- 222-813-1
- EC Name:
- 2,2'-[[5-acetamido-4-[(2-bromo-4,6-dinitrophenyl)azo]-2-methoxyphenyl]imino]diethyl diacetate
- Cas Number:
- 3618-72-2
- Molecular formula:
- C23H25BrN6O10
- IUPAC Name:
- 2-{[2-(acetyloxy)ethyl]({4-[ (E)-2-(2-bromo-4,6-dinitrophenyl)diazen-1-yl]-5acetamido-2-methoxyphenyl})amino}ethyl acetate
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Sprague-Dawley
- Sex:
- male/female
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- arachis oil
- Details on oral exposure:
- 10 ml/kg
All rats were dosed once only by gavage using a metal cannula attached to a graduated syringe. The
dose volume administered to each animal was calculated according to its fasted bodyweight at the
time of dosing. - Doses:
- 5000 mg/kg bw
- No. of animals per sex per dose:
- 5 males and 5 females
- Control animals:
- no
- Details on study design:
- A group of ten fasted rats (five males and five females) were dosed at the highest dose level used in
the range finding study which caused less than 50% mortalities.
Animals were observed 1 and 4 hours after dosing and subsequently once daily for 14 days. Mort
alities and evidence of overt toxicity were recorded at each observation. Individual bodyweights were
recorded on the day of dosing (day 0) and on days 7 and 14.
AlI animals were subjected to gross necropsy examination for any macroscopic abnormalities. No tis
sues were retained.
Results and discussion
Effect levelsopen allclose all
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- > 5 000 mg/kg bw
- Based on:
- test mat.
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- > 4 850 mL/kg bw
- Based on:
- act. ingr.
- Mortality:
- No mortality was recorded
- Clinical signs:
- irregular respiration
- Body weight:
- lower than 10% body weight loss
- Remarks:
- Normal bodyweight gains were recorded for all animals during the study period.
- Gross pathology:
- No abnormalities were seen at necropsy at the end of the study.
Applicant's summary and conclusion
- Interpretation of results:
- GHS criteria not met
- Conclusions:
- Under the study conditions, the nominal LC50 was determined to be >5000 mg/kg bw (i.e. >4870 mg
a.i./kg bw). - Executive summary:
A study was conducted to determine the acute oral toxicity of the test substance to rats according
to OECD Guideline 401. Sprague Dawley rats were administered a single dose of the test substance
(97.4% purity) in arachis oil via gavage. There were 5 male and 5 female rats per group, exposed to
a nominal concentration of 5000 mg/kg bw (equivalent to 4870 mg a.i./kg bw). The animals were then
observed during 14 days for mortality and clinical signs. Bodyweights were recorded on the day of dosing
and on Days 7 and 14. All animals were subjected to gross necropsy examination for macroscopic
abnormalities at test end. No tissues were retained. There was no mortality. Hunched posture and
piloerection were noted in alI treated animals on the day of dosing and on Day 1. All rats also showed
decreased respiratory rate and orange stained urine at the 4 h observation point. The clinical signs
resolved by Day 2. Normal bodyweight gains were recorded throughout the study and no abnormalities
were seen at necropsy. Under the study conditions, the nominal LD50 was determined to be >5000 mg/
kg bw (i.e. >4870 mg a.i./kg bw) (Archroma, 1986).
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