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Diss Factsheets
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EC number: 202-858-3 | CAS number: 100-50-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Skin irritation / corrosion
Administrative data
- Endpoint:
- skin irritation: in vitro / ex vivo
- Type of information:
- experimental study
- Adequacy of study:
- other information
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 012
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 431 (In Vitro Skin Corrosion: Human Skin Model Test)
- Qualifier:
- according to guideline
- Guideline:
- EU Method B.40 (In Vitro Skin Corrosion: Transcutaneous Electrical Resistance Test (TER))
- Principles of method if other than guideline:
- The experiment was carried out on a reconstructed human epidermis EST-1000 (CeIlSystems, St. Katharinen, Germany).
- GLP compliance:
- yes
Test material
- Reference substance name:
- Cyclohex-3-ene-1-carbaldehyde
- EC Number:
- 202-858-3
- EC Name:
- Cyclohex-3-ene-1-carbaldehyde
- Cas Number:
- 100-50-5
- Molecular formula:
- C7H10O
- IUPAC Name:
- cyclohex-3-ene-1-carbaldehyde
- Details on test material:
- content 97.7 %
Constituent 1
In vitro test system
- Test system:
- human skin model
- Remarks:
- EST-1000 (Cell Systems, St. Katharinen, Germany)
- Source species:
- human
- Cell type:
- other: reconstructed 3D human epidermis
- Vehicle:
- unchanged (no vehicle)
- Amount/concentration applied:
- 50 µL
- Duration of treatment / exposure:
- incubation periods of 3 min and 60 min
- Duration of post-treatment incubation (if applicable):
- no post-treatment incubation period; 3 hours incubation with MRR solution
- Number of replicates:
- 3
Results and discussion
In vitro
Resultsopen allclose all
- Irritation / corrosion parameter:
- % tissue viability
- Remarks:
- 3 min treatment
- Value:
- 43.65
- Remarks on result:
- other: mean of 3 samples compared to negative control (NaCL, 0.9%, set to 100% viability)
- Irritation / corrosion parameter:
- % tissue viability
- Remarks:
- 60 min treatment
- Value:
- 5.25
- Remarks on result:
- other: mean of 3 samples compared to negative control (NaCL, 0.9%, set to 100% viability)
Any other information on results incl. tables
Sample No | Test item | Time [min.] | OD mean* | StdDev | Viability % |
1 -3 | control NaCl 0.9% | 60 | 2.08 | 0.10 | 100 |
4 -6 | Tetrahydrobenzaldehyd | 60 | 0.11 | 0.01 | 5.25 |
13 -15 | control NaCl 0.9% | 3 | 3.0 | 0.10 | 100 |
16 -18 | Tetrahydobenzaldehyd | 3 | 1.31 | 0.35 | 43.65 |
* 6 values
Applicant's summary and conclusion
- Interpretation of results:
- Category 1A (corrosive) based on GHS criteria
- Conclusions:
- Tetrahydrobenzaldehyde was characterised by a significant impact on cell viability after 3 min. or after the 60 min. period.
Thus, it should be labelled as corrosive to skin. - Executive summary:
A study for predicting non-specific, corrosive potentials of compounds by using reconstructed human skin (RHS) was performed. The experiment was carried out on a reconstructed human epidermis EST-1000 (CellSystems, St. Katharinen, Germany) for detection of topically applied skin corrosives with the test item dibutyl hydrogen phosphate.
Corrosive skin effects of substances are defined as irreversible damage of skin; namely, visible necrosis through the epidermis and into the dermis, following the application of a test substance for up to four hours. In vivo corrosive reactions are typified by ulcers, bleeding, and bloody scabs.
A 100% concentration was tested on the skin/epidermal equivalents in triplets. For the determination of time related cytotoxic effects the incubation periods were 3 min. and 60 min., respectively. By this the study, was conducted in accordance with the OECD 431 guideline using the test item concentrations and incubation periods recommended there. These tests are also related to the revised OECD 404 guideline "Acute Dermal Irritation/Corrosion".
The test item was applied at a 100% concentration, i.e. 50µl per insert.
The MTT (Methylthiazoletetrazolium) method has determined the following values of viability after 3 min. or after 60 min. of incubation: 44% and 5% (rounded), respectively.
Thus, the results show that a corrosive property of the test item was determined by the assay used.
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