Phenol, 4-[(1E,3S)-3-ethenyl-3,7-dimethyl-1,6-octadien-1-yl]-

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2010

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Alga, Growth Inhibition Test)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

4-[(1E,3S)-3-ethenyl-3,7-dimethylocta-1,6-dien-1-yl]phenol

EC Number:

685-515-4

Cas Number:

10309-37-2

Molecular formula:

C18H24O

IUPAC Name:

4-[(1E,3S)-3-ethenyl-3,7-dimethylocta-1,6-dien-1-yl]phenol

Test solutions

Vehicle:

yes

Details on test solutions:

Vehicle control: Tween 80

Test organisms

Test organisms (species):

Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

72 h

Test conditions

Test temperature:

22.4-22.9

pH:

7.89-8.54

Details on test conditions:

TEST SYSTEM
- Test vessel: 250 mL conical f1asks. The test containers were incubated at 21 to 24℃ with a continuous light intensity of 4440 to 8880 Lux.
- fill volume: 150 mL
- Initial cells density: 10000 cells/ml. Algal cells were counted at every 24 hours interval up to the end of the treatment period.
- No. of vessels per concentration (replicates): 6
- No. of vessels per control (replicates): 6
- No. of vessels per vehicle control (replicates): 6
OTHER TEST CONDITIONS
- Light intensity: 7310-7640 Lux

TEST CONCENTRATIONS
- Range finding study
- Test concentrations: 1, 2.5, 5 mg/L
- Results used to determine the conditions for the definitive study: 5 mg/L (limit of solubility)

Reference substance (positive control):

yes

Remarks:

Potassium dichromate

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

> 2.108 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

test mat.

Basis for effect:

growth rate

Effect concentrations 2

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

> 2.108 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

test mat.

Basis for effect:

growth rate

Details on results:

As the percent change in analysed concentration over nominal concentration at the end of test was > 20 %, the results were expressed as initial measured concentration (i.e. 2.1 085 mg/L).

Reported statistics and error estimates:

Statistical analysis was not carried out as there was no inhibition of growth at the limit concentration tested.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes