4-Piperidinone, 2,6-diethyl-2,3,6-trimethyl-1-(1-phenylethoxy)-

Adsorption / desorption

Administrative data

Endpoint:

adsorption / desorption: screening

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2008

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Study performed according to OECD- and EEC-Guideline under GLP

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2008

Report date:

2008

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 121 (Estimation of the Adsorption Coefficient (Koc) on Soil and on Sewage Sludge using High Performance Liquid Chromatography (HPLC))

Deviations:

no

GLP compliance:

yes

Type of method:

HPLC estimation method

Media:

soil/sewage sludge

Test material

Test material information

Constituent 1

Details on test material:

Identification: TKA 40270 (CGPS 345)
Batch Number: Lot 02243FC7, Op. 1/07
Purity: Confidential Information (see Analytical Certificate, study no. 38291-1)
Appearance: Liquid, Colorless to yellow
Expiry Date: October 01, 2012
Storage Conditions: At room temperature at about 20 °C. Storage at under 18 °C may lead to partial solidification. To reliquify, heat content to 25-35 °C. Protect from light.
As the test item is light sensitive, all test item handling was performed under red light.

Radiolabelling:

not specified

Study design

Test temperature:

RT

HPLC method

Details on study design: HPLC method:

Apparatus: Merck-Hitachi autosampler L-7200
Merck pump L-6200
Merck UV/VIS detector L-4200
Column: LiChrospher 100 CN, particle size 5 µm, 250 mm x 4 mm
Mobile Phase: Water / methanol (45:55; v/v); pH 6.8
Detection: Test item: 218 nm
Reference items: 218 nm
Sodium nitrate: 218 nm
Temperature: RT °C
Injection Volume: 10 µL
Flow: 1.0 mL/min
Run Time: 30 minutes
Retention Times: 7.51

Batch equilibrium or other method

Analytical monitoring:

yes

Details on sampling:

- Concentrations: 115.02 µg/mL
- Sampling interval: injected three times.
- Sample storage before analysis: No data

Details on test conditions:

38.34 mg TKA 40270 (CGPS 345) was dissolved in acetonitrile (100 mL) to prepare a stock
solution of 383.4 µg/mL. The stock solution (3 mL) was diluted with a solvent mixture of
methanol and water (55:45; v/v) to 10 mL to obtain a test solution of 115.02 µg/mL. The pH
value of the test item solution was determined to be 6.8. This test item solution was injected
three times.

Duration of adsorption equilibration

Sample No.:

#3

Duration:

7.53 min

Initial conc. measured:

11.5 mg/kg sludge solids

pH:

6.8

Temp.:

22 °C

Results and discussion

Adsorption coefficient

Adsorption coefficient 1

Type:

Koc

Value:

14 824

Adsorption coefficient 2

Type:

log Koc

Value:

4.2

Results: HPLC method

Details on results (HPLC method):

Stock solutions of the reference items were prepared as described:
10.94 mg of acetanilide, 10.89 mg of isoproturon, 10.15 mg of linuron, 11.37 mg of pyrazophos,
11.17 mg of diclofop-methyl and 10.49 mg of 2,4-DDT were each dissolved in 10 mL of
methanol.
A combined reference solution was prepared by transferring 500 µL of each stock solution
(acetanilide, isoproturon, linuron, pyrazophos, diclofop-methyl and 2,4-DDT) into one
volumetric flask and diluting to a volume of 5 mL with a solvent mixture of methanol and water
(55:45; v/v). This reference solution was injected six times.
Sodium nitrate (10.93 mg) was dissolved in 10 mL of methanol to obtain a stock solution. The
stock solution (100 µL) was diluted to 1 mL with a solvent mixture of methanol and water
(55:45; v/v). This sodium nitrate solution was injected six times for the determination of the dead
time of the HPLC system.

Results: Batch equilibrium or other method

Adsorption and desorption constants:

Not applicable.

Recovery of test material:

Not applicable

Concentration of test substance at end of adsorption equilibration period:

Not applicable

Concentration of test substance at end of desorption equilibration period:

Not applicable

Details on results (Batch equilibrium method):

Not applicable

Statistics:

Not applicable

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The log of the adsorption coefficient (KOC) of TKA 40270 (CGPS 345) was estimated to be
log KOC 4.2 which is equal to a KOC value of 14824 using the HPLC method. This value indicates that TKA 40270 (CGPS 345) will be adsorbed by
organic carbon in soil. TKA 40270 (CGPS 345) can be classified to be immobile.

Executive summary:

The test item solution was injected three times and the combined reference solution was injected six times. The log KOC of the test item was calculated using a regression curve (log k' vs. log KOC) and was found to be 4.2, which is equal to a KOC value of 14824. In the present study the reference items covered the range of log KOC 1.25 to log KOC 5.63.

The chromatography of the test item resulted in one sharp main peak with a well-defined and reproducible retention time. Based on the chromatographic data, TKA 40270 (CGPS 345) was considered to be stable during the test procedure.

The capacity factor (k') was calculated for each reference item using the retention times. Log KOC was plotted versus log k' for each component to obtain a regression curve. The tabulated values represent rounded mean results, which were obtained by calculation using the exact raw data.