Sodium dicyanoamide

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1976-01-28

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Acceptable for reliability but not in detail documented. Study report meets basic scientific principles. Study was conducted prior to GLP and OECD guideline implementation.

Cross-reference

Reason / purpose for cross-reference:

reference to same study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1976

Report date:

1976

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 401 (Acute Oral Toxicity)

Principles of method if other than guideline:

No further information available.

GLP compliance:

no

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Dicyanamide sodium salt

IUPAC Name:

Dicyanamide sodium salt

Test material form:

other: solid

Details on test material:

The test material was suspended in propylene glycol.

Test animals

Species:

rat

Strain:

not specified

Sex:

male/female

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

propylene glycol

Doses:

250 mg/kg
500 mg/kg
630 mg/kg
800 mg/kg
1000 mg/kg
2000 mg/kg

No. of animals per sex per dose:

5 male and 5 female were used for each dose level.

Control animals:

no

Results and discussion

Effect levels

Effect levels 1

Sex:

male

Dose descriptor:

LD50

Effect level:

725 mg/kg bw

Based on:

test mat.

95% CL:

605 - 875

Effect levels 2

Sex:

female

Dose descriptor:

LD50

Effect level:

775 mg/kg bw

Based on:

test mat.

95% CL:

670 - 895

Any other information on results incl. tables

Nasal hemorrhage, sluggish locomotion and ruffled, unkempt coats were noted at 250 mg/kg. At dosage level of 500 mg/kg, 630 mg/kg and 800 mg/kg, the animals were lethargic with ruffled, unkempt coats. Spasmodic movements were noted. Within on hour, all animals were in a fetal position. Lacrimation, ocular and nasal hemorrhage were noted. Convulsions began approximately two hours after dosing. The animals dosed at 1000 mg/kg and 2000 mg/kg showed the same toxicity as at 800 mg/kg. All animals died within 2 -4 hours after incubation.

Applicant's summary and conclusion

Interpretation of results:

Toxicity Category IV

Remarks:

Migrated information Criteria used for interpretation of results: EU

Conclusions:

LD50 value of the test item is 725 mg/kg (male albino rats), therefore the test item will be classified as acute toxic; category 4; oral, H302.

Executive summary:

The study was performed 1976, before GLP- and OECD-testing guidelines were available and in force.

In this assay toxicity was recorded . LD50 value of the test item is 725 mg/kg (male albino rats), therefore the test item will be classified as

acute toxic; category 4; oral, H302 according to GHS/CLP classification criteria.