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EC number: 223-095-2 | CAS number: 3734-33-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Endpoint summary
Administrative data
Description of key information
Additional information
Short-term toxicity to fish:
Fish Acute Toxicity test according to OECD Guideline 203 was conducted for Denatonium benzoate (granular form). The nominal concentration selected for the experiment were 6.25 mg/L, 12.5 mg/L, 25 mg/L, 50 mg/L & 100 mg/L and Zebra fishes were exposed to these concentration for 96 hours. The lethal concentrations LC50 was found to be > 100mg/L indicatingthat denatonium benzoate isnot likely to exhibit toxicity to fish as per the CLP criteria.
Short-term toxicity to aquatic invertebrates:
No. of studies of target substance denatonium benzoate (CAS No 3734-33-6)reviewed for Short-term toxicity to aquatic invertebrates from reliable sources having Klimisch rating 4.
The summary of the results are presented below:
Sr.No |
Endpoint |
Effect values |
Basis for effects |
Duration |
Species |
Sources |
1 |
LC50 |
>500 mg/L |
Mortality |
24 hrs. |
Daphnia magna |
Study report |
LC50 |
>500 mg/L |
Mortality |
48 hrs. |
|||
NOEC |
50 mg/L |
Appearance of a white line on their back |
48 hrs. |
|||
2 |
LC50 |
400 mg/L |
Mortality |
96 hrs. |
Crangon sp. |
Study report |
By applying weight of evidence approach to the target substancedenatonium benzoate, the endpoint value was found to vary between LC50= 400 to >500 mg/l in a 24, 48 & 96 Hours respectively. Study on the basis of mortality effect. Thus considering the CLP Criteria for aquatic classification of the substance, it is concluded that substance does not exhibit short term toxicity to aquatic invertebrate (Daphnia magna).
Toxicity to aquatic algae and cyanobacteria:
The effect of test substance Denatonium Benzoate (granular form), CAS No. 3734-33-6 was studied on the growth of fresh water green alga Chlorella vulgaris. The study was conducted following OECD guideline 201- Alga growth inhibition test. The test concentration chosen for the study were 6.25 mg/l, 12.5 mg/l, 25mg/l, 50 mg/l, 100mg/l and 200 mg/l. The test concentrations were prepared using stock solution of the test substance using mineral media. The green alga was exposed to the test concentration for a period of 72 hours to observe average specific growth rate and % growth inhibition under the effect of test substance. EC50 calculated graphically through probit analysis was observed to be 281.556mg/l indicating that denatonium benzoate is not likely to exhibit toxicity to aquatic algae as per the CLP criteria.
Toxicity to microorganisms
The toxicity of Denatonium benzoate to micro-organisms was estimated using QSAR Toolbox version 3.3
The EC50 value of Denatonium benzoate in Vibrio fischeri during a 15 minute exposure was estimated to be 511.58 mg/L based on the effects on behaviour of the organisms. This value indicates that the substance is not likely to exhibit toxicity to micro-organisms.
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