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Diss Factsheets
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EC number: 942-924-5 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: see 'Remark'
- Remarks:
- GLP guideline study: The results in this study were consistent with the validity criteria with one exception. The mean oxygen uptake in the blank controls was lower than 20 mg/g*h (17 mg/g*h). The reference substance shows an EC50 in the specified range (usual range of EC50 of the last 20 values in the laboratory was 6.0 - 14.1 mg/L). The used activated sludge which was collected from the same waste water treatment plant showed a maximum oxygen consumption of 36.8 mg/g*h and minimum oxygen consumption of 14.5 mg/g*h of the last 20 measurements. For this the study is classified as valid.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: Direct addition by initial weight into the test flasks. The test substance was weighed in the required amounts for the test concentrations directly to the test vessels. - Test organisms (species):
- activated sludge, domestic
- Details on inoculum:
- - Laboratory culture: yes, Municipal activated sludge taken from a laboratory wastewater treatment plant (Mannheim, Germany) fed with municipal sewage.
- Preparation of inoculum for exposure: After arrival of the activated sludge suspension in the test facility the suspension was sieved with a fine woven mesh (mesh size about 1 mm). This suspension was pre-aerated over night at room temperature. At the next day the sludge suspension was washed once with drinking water and the suspension was adjusted to 3 g/L Dw. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Test temperature:
- 21.5 - 22 °C
- pH:
- 7.5 - 7.6
- Dissolved oxygen:
- Oxygen concentration during aeration: > 2 mg/L
Oxygen concentration immediately before measurement: > 7 mg/L - Nominal and measured concentrations:
- Nominal: 62.5, 125, 250, 500 and 1000 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 1 L glass beakers with 500 mL test solution
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 6
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: according to OECD guideline
- Intervals of water quality measurement:
OTHER TEST CONDITIONS
- Adjustment of pH: no
EFFECT PARAMETERS MEASURED: After the 3h incubation time the oxygen uptake was measured for a period of about 8 to 10 minutes or until the oxygen concentration fell below 2 mg/L. - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC10
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Results with reference substance (positive control):
- According to the test guideline OECD 209 the EC50 values of the reference substance 3,5-dichlorophenol should be in the range of 2-25 mg/L after 3 hours of incubation. The EC50 of the reference substance 3,5-dichlorophenol was in the range of 2-25 mg/L in 3 hours. These results indicate that the microorganisms are responding normally to toxicant stress.
- Validity criteria fulfilled:
- yes
- Remarks:
- The results in this study were consistent with the validity criteria with one exception: oxygen uptake in the blank controls < 20 mg/g*h (=17 mg/g*h). However, the study is classifed as valid (see rational of reliability).
- Conclusions:
- No toxicity of the test substance to activated sludge up to a nominal test concentration of 1000 mg/L was observed. Thus, the EC10 (3h) is determined to be > 1000 mg/L.
Reference
Description of key information
EC10 (3h) > 1000 mg/L (nominal) for activated sludge (OECD 209)
Key value for chemical safety assessment
Additional information
One study investigating the toxicity of Hydrocarbons, C11-C13 (odd numbered), n-alkanes, <2% aromatics to aquatic microorganisms is available. The study was performed according to OECD guideline 209 under GLP conditions (BASF 2014). Nominal test substance concentrations between 62.5 and 1000 mg/L were tested by direct adding the substance to the test vessels. No respiration inhibition was observed in any treatment compared to the control. Thus, the 3h-EC10 value is determined to be > 1000 mg/L based on the nominal concentration.
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