Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 434-230-1 | CAS number: 144413-22-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: GLP, Guideline study however the test material was observed to be unstable to light conditions in the culture media and a decline in test concentration in the test solutions was observed over the test period.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 999
- Report date:
- 1999
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- GLP compliance:
- yes (incl. QA statement)
Test material
Reference
- Name:
- Unnamed
- Type:
- Constituent
- Details on test material:
- Sponsors identification: ARAKAWA KE-604
Description: pale yellow blocks
Batch No.: R61132
Purity: no data
Sampling and analysis
- Analytical monitoring:
- yes
Test solutions
- Vehicle:
- yes
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: 100 mg of the test substance was dissovled in dimethylformamide and the volume adjusted to 10ml to give a 100mg/10ml solvent stock solution. An aliquot (20µl) of this stock solution was dispersed in algal suspension (200 ml) to give a test concentration of 1.0 mg/l.
- Controls: negative and solvent
- Chemical name of vehicle: dimethylformamide
- Concentration of vehicle in test medium: 1.0 mg/l.
Test organisms
- Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- TEST ORGANISM
- Common name: green algae
- Strain: Scenedesmus subspicatus strain CCAP 276/20.
- Source (laboratory, culture collection): Culture Centre tor Algae and Protozoa (CCAP), Institute of Freshwater Ecology, Ferry House, Ambieside, Cumbria.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 72 h
Test conditions
- Test temperature:
- 24 ± 1 °C
- pH:
- 8.5 - 8.6
- Nominal and measured concentrations:
- Analysis of the test preparations (filtered and unfiltered) at 0 hours showed the measured test concentrations to be near nominal. Analysis of the test preparations at 72 hours showed a marked decline in measured test concentrations to 18% in the unfiltered the filtered samples and to 21% to 23% in the filtered samples.
- Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Material, fill volume: 250 ml glass conical flasks loosely covered with aluminium foil to reduce evaporation. Each vessel contained 100 ml of solution
- Aeration: no
- Renewal rate of test solution (frequency/flow rate): none
- Initial cells density: 10000 algal cells per ml.
- No. of vessels per concentration (replicates): 6
- No. of vessels per control (replicates): 3
- No. of vessels per vehicle control (replicates): 3
GROWTH MEDIUM
- Standard medium used: yes
TEST MEDIUM / WATER PARAMETERS
The culture medium was prepared using reverse osmosis purified deionised water (EIga Optima 15+) and the pH adjusted to 7.5 ± 0.1 with 0.1N NaOH or HCI. The prepared media was sterilised by 0.22 pm membrane filtration and stored in darkness.
OTHER TEST CONDITIONS
- Light intensity and quality: continuous illumination (intensity approximately 7000 lux) and constantly shaken at 100 rpm for 72 hours.
TEST CONCENTRATIONS
- Justification for using less concentrations than requested by guideline: Based on the result of the range-finding study a “limit test“ was conducted for the definitive study at a test concentration of 1.0 mg/l to confirm that at the highest attainable test concentration of 1.0 mg/l, no effect on algal growth was observed.
- Range finding study
- Test concentrations: 1.0 mg/l
- Results used to determine the conditions for the definitive study: 1.0 mg/l. - Reference substance (positive control):
- yes
- Remarks:
- 100µl/l dimethylformamide
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 0.49 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.49 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
Any other information on results incl. tables
Chemcial Analysis:
Analysis of the test concentrations (filtered and unfiltered) at 0h showed the measured test concentrations to be near nominal. Analysis of the test concentrations at 72 h showed a marked decline in the measured test concentrations to 18 % in the unfiltered samples and to 21 - 23 % in the filtered samples. The decline in the measure test concentrations over the study period, in line with the pre-study stability analysis, is considered to be due to the photocatalysed reaction of the test material with the salts/ complexing agents in the test media.
In order to give a worst case analysis of the data, it was considered justifiable to base the results on the time weighted mean of the measured test concentrations of the filtered test samples.
Nominal concentration(mg/l) |
Time-weighted mean measured test concentration (mg/l) |
Expressed as a % of the nominal concentration |
1.0 |
0.493 |
49 |
The following results are based on the time weighted mean measured test concentration of the filtered test samples.
EbC50 (72h): >0.49mg/l
ErC50 (72h): >0.49mg/l
NOEC = 0.49 mg/l
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.