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EC number: 292-587-7 | CAS number: 90640-66-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: dermal
Administrative data
- Endpoint:
- acute toxicity: dermal
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1974-1975
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- test procedure in accordance with national standard methods with acceptable restrictions
- Remarks:
- Short summary of acute dermal toxicity study, no GLP, no data on the composition of the test substance
Cross-referenceopen allclose all
- Reason / purpose for cross-reference:
- reference to same study
Reference
- Endpoint:
- acute toxicity: inhalation
- Type of information:
- experimental study
- Adequacy of study:
- disregarded due to major methodological deficiencies
- Study period:
- 1974-1975
- Reliability:
- 3 (not reliable)
- Rationale for reliability incl. deficiencies:
- unsuitable test system
- Remarks:
- Short summary of acute inhalation study, saturated vapor concentration test, no GLP, no information on the composition of the test substance
- Reason / purpose for cross-reference:
- reference to same study
- Reason / purpose for cross-reference:
- reference to same study
- Reason / purpose for cross-reference:
- reference to same study
- Reason / purpose for cross-reference:
- reference to same study
- Reason / purpose for cross-reference:
- reference to same study
- Qualifier:
- no guideline available
- Principles of method if other than guideline:
- Saturated vapour was generated at 22°C by spreading 50-100 g of chemical over a 200 cm² area on a shallow tray placed near the top of a 120-L plexiglass chamber which is then sealed for at least 16 h while an intermittently operated fan agitated the internal chamber atmosphere. Rats were then introduced in a gasketed drawer-type cage designed and operated to minimize vapour loss.
- GLP compliance:
- no
- Test type:
- other: saturated vapor concentration test
- Limit test:
- yes
- Species:
- rat
- Strain:
- not specified
- Sex:
- not specified
- Route of administration:
- inhalation: vapour
- Type of inhalation exposure:
- whole body
- Vehicle:
- other: unchanged (no vehicle)
- Analytical verification of test atmosphere concentrations:
- no
- Duration of exposure:
- 8 h
- Concentrations:
- Rats were exposed to a static test atmosphere generated following a 16-h period in which the liquid was allowed to evaporate at 22rees C. It is not clear whether the maximum attainable vapour concentration had been reached as no analytical measurements were done.
- No. of animals per sex per dose:
- 6 (sex unknown)
- Control animals:
- no
- Details on study design:
- - Duration of observation period following administration: 14 days
- Sex:
- not specified
- Dose descriptor:
- LC50
- Exp. duration:
- 8 h
- Remarks on result:
- not determinable because of methodological limitations
- Mortality:
- No mortality occurred
- Clinical signs:
- other: Animals showed no clinical signs
- Body weight:
- Animals showed normal BW gain
- Gross pathology:
- Nothing remarkable at necropsy
- Interpretation of results:
- study cannot be used for classification
- Conclusions:
- A static test atmosphere generated as a vapour over a 16-h period was not toxic to rats. It should, however, be noted that this corresponds to a very low concentration of maximally 0.00147 mg/L.
- Executive summary:
A saturated vapour was generated at 22 degrees C by spreading 50 -100 g of the test substance, tetraethylenepentamine, over a 200 cm2 area on a shallow tray placed near the top of a 120-L plexiglass chamber which was then sealed for at least 16 h while an intermittently operated fan agitated the internal chamber atmosphere. A group of 6 rats was then introduced in a gasketed drawer-type cage designed and operated to minimize vapour loss. They were exposed for 8 h. Animals showed no clinical signs and normal BW gain. Nothing remarkable was observed at necropsy 14 days after exposure. Taking into account a vapour pressure of 0.0189 Pa (IUCLID Section 4.6) and a MW of 189.3, the maximum vapour concentration was calculated to be 0.00147 mg/L, which is very low concentration. This study, therefore, cannot be used for classification and labelling purposes.
- Reason / purpose for cross-reference:
- reference to same study
Reference
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1974-1975
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- test procedure in accordance with national standard methods with acceptable restrictions
- Remarks:
- The study was performed pre-GLP and pre-guideline. Limited reporting. No information on the composition or purity of the test substance.
- Reason / purpose for cross-reference:
- reference to same study
- Reason / purpose for cross-reference:
- reference to same study
- Reason / purpose for cross-reference:
- reference to same study
- Reason / purpose for cross-reference:
- reference to same study
- Reason / purpose for cross-reference:
- reference to same study
- Qualifier:
- no guideline available
- Principles of method if other than guideline:
- 5 non-fasted animals per group were dosed by a single gavage application. Observation period was 14 days.
- GLP compliance:
- no
- Remarks:
- Before GLP
- Test type:
- standard acute method
- Limit test:
- no
- Species:
- rat
- Strain:
- Wistar
- Sex:
- male
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Age at study initiation: stated to be 3 -4 weeks old
- Weight at study initiation: stated to be 90 -120 g - Route of administration:
- oral: gavage
- Vehicle:
- unchanged (no vehicle)
- Doses:
- 2.0, 4.0 and 8.0 mL/kg
The values were converted based on the given relative density of 0.991 - 0.9994. The following doses were calculated based on the lowest relative density value: 1982, 3964 and 7928 mg/kg bw. - No. of animals per sex per dose:
- 5 males/dose
- Control animals:
- no
- Details on study design:
- No details available
- Sex:
- male
- Dose descriptor:
- LD50
- Effect level:
- 3.25 mL/kg bw
- Based on:
- test mat.
- 95% CL:
- >= 2.36 - <= 4.47
- Remarks on result:
- other: LD50 calculated by the moving average method; LD50 = 3.25 mL/kg bw corresponds to 3221 mg/kg bw.
- Mortality:
- 0/5, 4/5 and 5/5 at 2, 4 and 8 mL/kg bw, respectively.
- Clinical signs:
- other: - 2 mL/kg bw: no signs - 4 mL/kg bw: slightly sluggish 6 min, prostrate 2 h, death 2 -3 h - 8 mL/kg bw: sluggish 2 min, prostrate 30 min, death 2 -3 h
- Gross pathology:
- - lungs with petechiae, liver and spleens mottled, stomachs liquid-filled and red, intestines distended and liquid-filled and red or slightly yellow, dark kidneys, bladders fluid-filled (in victims)
- livers mottled (in survivors) - Interpretation of results:
- other: CLP/EU GHS criteria are not met, no classification required according to Regulation (EC) No 1272/2008
- Conclusions:
- Because of the LD50 value of 3221 mg/kg bw the test material is not considered to be harmful.
- Executive summary:
Five male rats per group were dosed by intubation with the undiluted test substance. Three groups were used; dose levels were 2.0, 4.0 and 8.0 mL/kg bw. 0/5, 4/5 and 5/5 of the animals died, respectively. The LD50 was calculated to be 3.25 mL/kg bw (3221 mg/kg bw) with 95% confidence limits of 2.36 and 4.47 mL/kg bw. , indicating classification in OECD-GHS category V.
- Reason / purpose for cross-reference:
- reference to same study
Reference
- Endpoint:
- skin irritation: in vivo
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- 1974-1975
- Reliability:
- 4 (not assignable)
- Rationale for reliability incl. deficiencies:
- documentation insufficient for assessment
- Remarks:
- available, no GLP, no data on composition of the test substance
- Reason / purpose for cross-reference:
- reference to same study
- Reason / purpose for cross-reference:
- reference to same study
- Reason / purpose for cross-reference:
- reference to same study
- Reason / purpose for cross-reference:
- reference to same study
- Reason / purpose for cross-reference:
- reference to same study
- Qualifier:
- no guideline available
- Principles of method if other than guideline:
- Chemical was applied in 0.01 mL amounts to clipped uncovered intact skin of 5 rabbit bellies either undiluted or in progressive dilutions of 10, 1, 0.1 or 0.01% in solvent. One of 10 grades were assigned based on appearance of moderate or marked capillary injection, erythema, edema or necrosis within 24 h. No injury from undiluted is considered grade 1.
- GLP compliance:
- no
- Remarks:
- Before GLP
- Species:
- rabbit
- Strain:
- not specified
- Type of coverage:
- open
- Preparation of test site:
- clipped
- Vehicle:
- water
- Controls:
- no
- Duration of treatment / exposure:
- Not indicated, because of the limited volume used and the open application it is assumed that the test substance stayed there and was not removed at all.
- Observation period:
- Not indicated, at least up to 24 h.
- Number of animals:
- 5
- Irritation parameter:
- erythema score
- Basis:
- mean
- Time point:
- other: 24 h
- Score:
- 6
- Max. score:
- 10
- Reversibility:
- no data
- Irritant / corrosive response data:
- Only a description of the results was given:
- Undiluted test substance: moderate erythema (1/5), marked erythema (1/5), moderate necrosis (3/5)
- 10% dilution: no irritation (5/5) - Other effects:
- No info
- Interpretation of results:
- study cannot be used for classification
- Conclusions:
- Because 3 out of 5 rabbits showed moderate necrosis following treatment with the undiluted test substance, classification in Cat. 1 can be assumed. Because of unknown exposure duration and unkown observation period, classification in subcategories (1A, 1B, 1C) is not possible.
- Executive summary:
The belly of each of 5 rabbits was clipped, the test substance, Amines, polyethylenepoly-, tetraethylenepentamine fraction, was applied undiluted to the uncovered intact skin at a volume of 0.01 mL. The skin was observed for at least up to 24 h. One rabbit showed moderate erythema, a second rabbit showed marked erythema whereas the other 3 showed moderate necrosis. A 10% dilution in distilled water did not show irritation. Because 3 out of 5 rabbits showed moderate necrosis following treatment with the undiluted test substance, classification in OECD-GHS Cat. 1 is assumed. Because of unknown exposure duration and unkown observation period, classification in subcategories (1A, 1B, 1C) is not possible.
- Reason / purpose for cross-reference:
- reference to same study
Reference
- Endpoint:
- eye irritation: in vivo
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- 1974-1975
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
- Remarks:
- Short summary of acute oral toxicity test, no GLP, no data on composition of the test substance
- Qualifier:
- no guideline available
- Principles of method if other than guideline:
- Eyes not staining with 5% fluorescein in 20 sec contact were accepted. Single installation of 0.005, 0.02, 0.10 or 0.5 mL undiluted or 0.5 mL of 40, 15, 5 or 1% dilutions were made into conjunctival sac of 5 rabbits. Read immediately unstained and after fluorescein at 24 h, with one of 10 grades assigned. Trace or no injury from 0.5 mL undiluted is grade 1.
- GLP compliance:
- no
- Remarks:
- Before GLP
- Species:
- rabbit
- Strain:
- not specified
- Vehicle:
- unchanged (no vehicle)
- Controls:
- no
- Duration of treatment / exposure:
- Single instillation
- Observation period (in vivo):
- No info, at least up to 24 h.
- Number of animals or in vitro replicates:
- 5
- Irritation parameter:
- cornea opacity score
- Basis:
- mean
- Time point:
- other: 24 h
- Score:
- 5
- Max. score:
- 10
- Reversibility:
- not specified
- Remarks on result:
- other: moderate corneal injury
- Interpretation of results:
- study cannot be used for classification
- Conclusions:
- Because 0.02 mL of the undiluted test substance caused moderate corneal injury, it is expected that the amount required according the current OECD guideline (0.1 mL) will induce more severe eye injury and the test substance is therefore considered to be at least 'highly irritating'.
- Executive summary:
The test substance, Amines, polyethylenepoly-, tetraethylenepentamine fraction, was applied undiluted at a volume of 0.02 mL to the conjunctival sac of 5 rabbits. Rabbits showed moderate corneal injury, 1/5 rabbits showed iritis. A volume of 0.005 mL per eye showed minor injury. Because a volume of 0.02 mL was used, it is expected that the amount required according the current OECD guideline (0.1 mL) will induce more severe eye injury and the test substance is therefore considered to be at least 'highly irritating'. Because of lack on info when using a volume of 0.1 mL, and on reversibility, classification in OECD-GHS categories is not possible.
Only a description of the results was given:
- 0.02 mL per eye: moderate corneal injury with iritis in 1/5 rabbits
- 0.005 mL per eye: minor injury
- Reason / purpose for cross-reference:
- reference to same study
Reference
- Endpoint:
- short-term repeated dose toxicity: oral
- Remarks:
- 7-day study
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- 1974-1975
- Reliability:
- 3 (not reliable)
- Rationale for reliability incl. deficiencies:
- significant methodological deficiencies
- Remarks:
- only limited observations, no GLP, no data on composition of the test substance, no analytical verification of the dose levels
- Reason / purpose for cross-reference:
- reference to same study
- Reason / purpose for cross-reference:
- reference to same study
- Reason / purpose for cross-reference:
- reference to same study
- Reason / purpose for cross-reference:
- reference to same study
- Reason / purpose for cross-reference:
- reference to same study
- Qualifier:
- no guideline available
- Principles of method if other than guideline:
- The test substance was added to ground Purina Chow and fed in the diet for 7 days. In the first part of the study 4 groups of Wistar rats were used, one control and 3 test groups. Because no effects were observed, a higher dose group and a control group were included in the second part of the study.
- GLP compliance:
- no
- Remarks:
- Before GLP
- Limit test:
- no
- Species:
- rat
- Strain:
- Wistar
- Sex:
- male/female
- Route of administration:
- oral: feed
- Vehicle:
- unchanged (no vehicle)
- Analytical verification of doses or concentrations:
- no
- Duration of treatment / exposure:
- 7 days
- Frequency of treatment:
- via the diet for 7 days
- Dose / conc.:
- 500 mg/kg bw/day (nominal)
- Remarks:
- first study
- Dose / conc.:
- 1 250 mg/kg bw/day (nominal)
- Remarks:
- first study
- Dose / conc.:
- 3 150 mg/kg bw/day (nominal)
- Remarks:
- first study
- Dose / conc.:
- 5 000 mg/kg bw/day (nominal)
- Remarks:
- second study
- No. of animals per sex per dose:
- 5
- Control animals:
- yes, plain diet
- Observations and examinations performed and frequency:
- Parameters examined:
- BW gain on days 1, 4 and 7
- Food intake
- Calculated dosage attained
- Absolute and relative liver and kidneys weight
- Mortality - Details on results:
- First study
Males: target 0.50 g/kg, 0.42 g/kg attained; target 1.25 g/kg, 1.05 g/kg attained; target 3.15 g/kg, 2.80 g/kg attained
Females: target 0.50 g/kg, 0.47 g/kg attained; target 1.25 g/kg, 1.26 g/kg attained; target 3.15 g/kg, 3.14 g/kg attained
Second study
Males: target 5.00 g/kg, 3.99 g/kg attained
Females: target 5.00 g/kg, 3.63 g/kg attained
Mortality: 0
Food intake: decreased at 5.00 g/kg bw
Body weight: body weight loss at 5.00 g/kg bw
Absolute and relative liver weight: statistically significantly decreased at 5.00 g/kg bw
Relative kidney weight: statistically significantly decreased at 5.00 g/kg bw. - Dose descriptor:
- NOAEL
- Effect level:
- 2 800 mg/kg bw/day (nominal)
- Based on:
- test mat.
- Sex:
- male
- Basis for effect level:
- other: Body and organ weight changes
- Dose descriptor:
- NOAEL
- Effect level:
- 3 140 mg/kg bw/day (nominal)
- Based on:
- test mat.
- Sex:
- female
- Basis for effect level:
- other: Body and organ weight changes
- Critical effects observed:
- no
- Conclusions:
- The NOAEL of this 7-day diet study, based on a limited numbers of parameters was 2.80 and 3.14 g/kg bw for males and females, respectively.
- Executive summary:
The test substance was added to the diet and fed to groups of 5 Wistar rats (30 days of age) per sex for 7 days. In the first part of the study 4 groups were used, these were dosed at 0, 0.50, 1.25 and 3.15 g/kg bw. Because no effects were observed on body weight gain, food intake and liver and kidneys weights, in the second part of the study, 2 groups were used, these were dosed at 0 and 5.00 g/kg bw. Dosages attained (calculated based on nominal levels and food intake) were: 0, 0, 0.42, 1.05, 2.80 and 3.99 g/kg bw for males, and 0, 0, 0.47, 1.26, 3.14 and 3.63 g/kg bw for females. Animals treated at the highest dose showed a decrease in food intake, body weight loss, and decreased absolute and relative liver weight and decreased relative kidney weight. The NOAELs, therefore, were, 2.80 and 3.14 g/kg bw for males and females, respectively.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 979
- Report date:
- 1979
Materials and methods
Test guideline
- Qualifier:
- no guideline available
- Principles of method if other than guideline:
- Rabbits were dosed using covered dermal application
- GLP compliance:
- no
- Remarks:
- Before GLP
- Test type:
- standard acute method
- Limit test:
- no
Test material
- Reference substance name:
- Amines, polyethylenepoly-, tetraethylenepentamine fraction
- EC Number:
- 292-587-7
- EC Name:
- Amines, polyethylenepoly-, tetraethylenepentamine fraction
- Cas Number:
- 90640-66-7
- Molecular formula:
- C8H23N5, C10H25N5
- IUPAC Name:
- (2-aminoethyl)[2-({2-[(2-aminoethyl)amino]ethyl}amino)ethyl]amine; bis(2-aminoethyl)({2-[(2-aminoethyl)amino]ethyl})amine
- Test material form:
- liquid
Constituent 1
Test animals
- Species:
- rabbit
- Strain:
- New Zealand White
- Sex:
- male
Administration / exposure
- Type of coverage:
- occlusive
- Vehicle:
- unchanged (no vehicle)
- Duration of exposure:
- 24-h contact with the test substance retained under impervious (polyethylene) sheeting on the clipped intact skin of the trunk.
- Doses:
- 1.0, 2.0 and 4.0 mL/kg bw
- No. of animals per sex per dose:
- 4 males at 1.0 and 2.0 mL/kg bw, 2 males at 4.0 mL/kg bw
- Control animals:
- no
Results and discussion
Effect levels
- Sex:
- male
- Dose descriptor:
- LD50
- Effect level:
- 1.26 mL/kg bw
- Based on:
- test mat.
- 95% CL:
- >= 0.772 - <= 2.06
- Remarks on result:
- other: LD50 calculated by the moving average method; 1.26 mL corresponds to about 1260 mg
- Mortality:
- 1/4, 4/4 and 2/2 at 1.0, 2.0 and 4.0 mL/kg bw, respectively
- Clinical signs:
- other: - 1.0 mL/kg bw: necrosis - 2.0 mL/kg bw: necrosis, prostate at 1 day - 4.0 mL/kg bw: necrosis
- Gross pathology:
- lungs and kidneys reddened in victims, no abnormalties in survivors
Applicant's summary and conclusion
- Interpretation of results:
- other: CLP/EU GHS Category 4 (H302) according to Regulation (EC) No 1272/2008
- Conclusions:
- Based on a LD50 value of 1.26 mL/kg bw which corresponds to about 1260 mg/kg bw, the test substance should be classified in Cat. 4 according to OECD-GHS
- Executive summary:
Two to four male rabbits per group were dosed by a 24 -h dermal application under occlusive conditions with the undiluted test substance. Three groups were used; dose levels were 1.0, 2.0, and 4.0 mL/kg bw. 1/4, 4/4 and 2/2 of the animals died, respectively. The LD50 was calculated to be 1.26 mL/kg bw with 95% confidence limits of 0.772 and 2.06 mL/kg bw, indicating classification in CLP/EU GHS Category 4.
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