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Diss Factsheets
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EC number: 204-679-6 | CAS number: 124-09-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
Description of key information
The test substance is soluble and appears to be caustic in aqueous solution. This last feature was critical in the study design of available reports and data.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 1 825 mg/L
Additional information
The acute toxicity of hexamethylene diamine was tested on several different recommended species of fish. Four study reports were available and input as endpoint summary records. The two last Endpoint Summary Records could not be assigned for reliability but were formerly included in the IUCLID4 of the OECD SIDS (2000).
Only three study reports were sufficiently detailed and solid enough to be scientifically reliable according to Klimisch scoring (Klimisch et al., 1997). Although one of these, the study of Cellier (1998) which tested the substance on Danio rerio under semi-static conditions, but without pH adjustment, cannot be considered as valid. Due to the caustic effect of the substance, test solutions had to be pH adjusted to the recommended range (6.5-8.5) or lethality would occur due to the pH of the test solution. Munk (1992) conducted a finely detailed study where Leuciscus idus were tested under static conditions, once without pH adjustment, then with pH adjustment. In the first trial, without adjustment, fish died after 1 hour of exposure at pH = 11.0 and 215 mg/L. In contrast when pH was adjusted to 8.5 prior to the test, no mortalities were observed at 215 mg/L, the highest concentration tested in this study. Finally, Dupont (1985) tested the acute toxicity of the substance on Pimephales promelas after pH adjustment of test solutions at very high concentration up to 10 000 mg/L under static conditions. No mortalities were observed up to 1 000 mg/L. The 96h-LC50 of 1825 mg/L
(95%IC = 1237-2461 mg/L) using Probit analysis was calculated. Despite the lack of chemical analysis and the fact the study was performed under static conditions, this is considered the key study for Fish as this is the only study in which a LC50 was determined at reasonable conditions of pH. Moreover, test solutions of HMD have been shown to be completely stable under environmentally relevant conditions in a recent study on algae.Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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