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EC number: 202-905-8 | CAS number: 100-97-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1979
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: non GLP, non Guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- not specified
- GLP compliance:
- not specified
- Analytical monitoring:
- no
- Details on sampling:
- no sampling
- Vehicle:
- no
- Details on test solutions:
- not indicated
- Test organisms (species):
- Nitocra spinipes
- Details on test organisms:
- Not fed during the study.
Only adult animals , harvested from 3 to 6 weeks old cultures were used. - Test type:
- static
- Water media type:
- brackish water
- Limit test:
- no
- Total exposure duration:
- 96 h
- Post exposure observation period:
- no post exposure period
- Hardness:
- not indicated
- Test temperature:
- 10°C
- pH:
- 7.8
- Dissolved oxygen:
- not indicated
- Salinity:
- 0.7 %
- Nominal and measured concentrations:
- at least six concentrations with one control (not specified)
- Details on test conditions:
- N. spinipes (2x 10 animals) were tested in 15 ml standard laboratory test tubes containing 10 ml of brackish water at 10°C. Mortality was recorded after 96 h under a low power microscope with strong illuminations.
- Reference substance (positive control):
- no
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 92.5 g/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Remarks on result:
- other: 95% CL: 26.8 - 305.0
- Details on results:
- no details given
- Results with reference substance (positive control):
- no reference substance
- Reported statistics and error estimates:
- Graphical analysis according to Litchfield and Wilcoxon.
- Validity criteria fulfilled:
- yes
- Conclusions:
- The LC50 of methenamine in Nitocra spinipes (brackish water) was determined to be 36 g/L.
- Executive summary:
The 96 hr-acute toxicity of methenamine to Nitocra spinipes was studied under static conditions. Nitocra were exposed to control and at least 6 test concentrations for 96 hr. Mortality/immobilization were observed at test termination. The 96 hour LC50 was 92.5 g/L.
This study is classified as acceptable and satisfies the guideline requirements for an acute toxicity study with saltwater invertebrates.
Results Synopsis
Test Organism Age: adult
Test Type: Static
LC50: 92.5 g/L (95% C.I. : 26.8 to 305.0 g/L
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1980
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: non GLP
- Qualifier:
- according to guideline
- Guideline:
- other: ASTM Committee on Water Quality, 1979: Proposed standard practice for conducting static acute toxicity tests on process wastewaters with Daphnia.
- Deviations:
- not specified
- GLP compliance:
- not specified
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material:
Chemical was of reagent quality. - Analytical monitoring:
- no
- Details on sampling:
- no sampling
- Vehicle:
- no
- Details on test solutions:
- Reconstituted water used.
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- Obtained from Ann Arbor Biological Center.
Fed on a suspension of dry activated yeast and Purina Trout Chow. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Post exposure observation period:
- no post exposure period
- Hardness:
- 100 mg/l CaCO3
- Test temperature:
- not indicated
- pH:
- 7.6
- Dissolved oxygen:
- not indicated
- Salinity:
- freshwater was used
- Nominal and measured concentrations:
- nominal: 15, 20, 30 , 40 g/L
- Details on test conditions:
- The tests were conducted in replicates of four by placing five young daphnids (1-3 days old) into each of 250 ml Erlenmeyer flasks containing 200 ml of test solution. The number of organisms not showing movement within 30 sec were presumed dead and thus were removed. Readings were taken at 2, 8, 24, and 48 h.
- Reference substance (positive control):
- no
- Key result
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- 36 g/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Remarks on result:
- other: 95 % CL: 29.9 - 43.4
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 15 g/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Details on results:
- no details given
- Results with reference substance (positive control):
- no reference substance
- Reported statistics and error estimates:
- LC50 was calculated by probit analysis (slope of probit line: 5.29)
- Validity criteria fulfilled:
- yes
- Conclusions:
- The LC50 of methenamine in Daphnia magna (freshwater) was determined to be 36 g/L.
- Executive summary:
The 48 hr-acute toxicity of methenamine to Daphnia magna was studied under static conditions. Daphnids were exposed to control and test chemical at nominal concentration of 15, 20, 30 and 40 g/L for 48 hr. Mortality/immobilization were observed daily. The 48 hour LC50 was 36 g/L. The 48 hr NOEC based on mortality/immobilization was 15 g/L.
This study is classified as acceptable and satisfies the guideline requirements for an acute toxicity study with freshwater invertebrates.
Results Synopsis
Test Organism Age : 1-3 days
Test Type: Static
LC50: 36 g/L (95% C.I. : 29.9 to 43.4 g/L
NOEL: 15 g/L
Probit Slope: 5.29
Referenceopen allclose all
Description of key information
The LC50 of methenamine in Daphnia magna (freshwater) was determined to be 36 g/L. The LC50 of methenamine in Nitocra spinipes (brackish water, saltwater) was determined to be 92.5 g/L.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Dose descriptor:
- EC50
- Effect concentration:
- 36 000 mg/L
Marine water invertebrates
Marine water invertebrates
- Dose descriptor:
- EC50
- Effect concentration:
- 92 500 mg/L
Additional information
Key-Studies:
- The 48 hr-acute toxicity of methenamine to Daphnia magna (Walton, 1980) was studied under static conditions. Daphnids were exposed to control and test chemical at nominal concentration of 15, 20, 30 and 40 g/L for 48 hr. Mortality/immobilization were observed daily. The 48 hour LC50 was 36 g/L. The 48 hr NOEC based on mortality/immobilization was 15 g/L.
This study is classified as acceptable and satisfies the guideline requirements for an acute toxicity study with freshwater invertebrates.
- The 96 hr-acute toxicity of methenamine to Nitocra spinipes was studied under static conditions. Nitocra were exposed to control and at least 6 test concentrations for 96 hr. Mortality/immobilization were observed at test termination. The 96 hour LC50 was 92.5 g/L.
This study is classified as acceptable and satisfies the guideline requirements for an acute toxicity study with saltwater invertebrates.
Based on the available data, no toxicity towards fresh and saltwater invertebrates species even at concentrations > 92000 mg/L, which by far exceeded the recommended limit concentration of OECD guidelines (100 mg/L) clearly indicate, that methenamine is not toxic at all towords saltwater or freshwater invertebrates.
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