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EC number: 204-857-3 | CAS number: 127-68-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Endpoint summary
Administrative data
Description of key information
Skin irritation
The dermal irritation potential of test chemical was assessed in various experimental studies conducted on rabbits. The predicted data using Danish QSAR database has also been compared with the experimental data. Based on the available data for the key and supporting studies, it can be concluded that the test chemical is unable to cause skin irritation and thus considered as not irritating. Comparing the above annotations with the criteria of CLP regulation, it can be classified under the category “Not Classified”.
Eye irritation
An ocular irritation potential of target chemical was assessed in various experimental studies conducted on rabbits. Based on the available data for the key and supporting studies, it can be concluded that the test chemical is able to cause severe eye damage and thus can be considered as irritating. Comparing the above annotations with the criteria of CLP regulation, it can be classified under the category “Category 2”.
Key value for chemical safety assessment
Skin irritation / corrosion
Link to relevant study records
- Endpoint:
- skin irritation: in vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study with acceptable restrictions
- Justification for type of information:
- Data is from experimental study report.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 404 (Acute Dermal Irritation / Corrosion)
- Principles of method if other than guideline:
- A Draize Test was carried out for chemical Sodium 3-nitrobenzenesulphonate (CAS no: 127-68-4) to assess its skin irritation potential.
- GLP compliance:
- no
- Specific details on test material used for the study:
- Name of the test chemical: Sodium 3-nitrobenzenesulfonate
Molecular Formula:C6H5NO5S.Na
Molecular Weight: 225.1556 g/mol
Substance Type: Organic
Physical State: Solid - Species:
- rabbit
- Strain:
- Vienna White
- Details on test animals or test system and environmental conditions:
- Mean body weights for male animals 2.87 kg, female animals 2.97 kg
- Type of coverage:
- occlusive
- Preparation of test site:
- other: either intact or abraded
- Vehicle:
- water
- Controls:
- other: untreated skin of the same animal
- Amount / concentration applied:
- Concentration: 50%
- Duration of treatment / exposure:
- 24 hour(s)
- Observation period:
- 8 d
- Number of animals:
- 6
- Details on study design:
- After the application time, the skin was washed with water which may have contained a mild detergent. Animals were observed for 8 days and skin changes were observed on working days. Findings were recorded after 24, 72 hours and at the end of the observation period. Findings were graded as described in OECD test guideline 404.
- Irritation parameter:
- edema score
- Basis:
- mean
- Time point:
- other: 24 h
- Score:
- 1.2
- Max. score:
- 2
- Reversibility:
- fully reversible within: 72 h
- Remarks on result:
- other: intact skin
- Irritation parameter:
- erythema score
- Basis:
- mean
- Time point:
- other: 72 h
- Score:
- 1.3
- Max. score:
- 2
- Reversibility:
- fully reversible within: 8 d
- Remarks on result:
- other: intact skin
- Irritant / corrosive response data:
- All skin reactions were disappeared within 8 days.
- Interpretation of results:
- other: not irritating
- Conclusions:
- From the experimental results, it is concluded that Sodium 3-nitrobenzenesulphonate (CAS no: 127-68-4)was not irritating to the skin of white Vienna rabbits.
- Executive summary:
A Draize Test was carried out for chemical Sodium 3-nitrobenzenesulphonate (CAS no: 127-68-4) on skin of six white Vienna rabbits in accordance with OECD test guideline 404.
The chemical was applied to the intact or abraded skin of each rabbit at a dose of 50% of Sodium 3-nitrobenzenesulphonate in water for 24 hours under occlusive condition.
After the application time, the skin was washed with water which may have contained a mild detergent. Animals were observed for 8 days and skin changes were observed on working days. Findings were recorded after 24, 72 hours and at the end of the observation period. Findings were graded as described in OECD test guideline 404.
The chemical caused slight erythema and edema which were fully reversible within 8 days.
Since the observed effects were not persisted during the observed period, the chemical Sodium 3-nitrobenzenesulphonate (CAS no: 127-68-4) was considered as not irritating to the skin of white Vienna rabbits.
Reference
Endpoint conclusion
- Endpoint conclusion:
- no adverse effect observed (not irritating)
Eye irritation
Link to relevant study records
- Endpoint:
- eye irritation: in vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study
- Justification for type of information:
- Data is from experimental report
- Qualifier:
- according to guideline
- Guideline:
- other: as mentioned below
- Principles of method if other than guideline:
- A Draize Test was carried out for chemical Sodium 3-nitrobenzenesulphonate (CAS no: 127-68-4) to assess the eye irritation potential of six white Vienna rabbits.
- GLP compliance:
- no
- Specific details on test material used for the study:
- Name of the test chemical: Sodium 3-nitrobenzenesulfonate
Molecular Formula:C6H5NO5S.Na
Molecular Weight: 225.1556 g/mol
Substance Type: Organic
Physical State: Solid - Species:
- rabbit
- Strain:
- Vienna White
- Details on test animals or tissues and environmental conditions:
- Mean body weight of the rabbits: 2.7 kg for males and 2.64 kg for females
- Vehicle:
- unchanged (no vehicle)
- Controls:
- other: untreated eye of the same animal
- Amount / concentration applied:
- Amount applied: 0.1 ml
- Duration of treatment / exposure:
- 24 h
- Observation period (in vivo):
- 8 d
- Number of animals or in vitro replicates:
- 6
- Details on study design:
- The eyes were not washed out after 24 hours after substance application. Findings were graded as described in OECD test guideline 405.
Findings were recorded after 24, 48, 78 hours, and at the end of the observation period (8 days). - Irritation parameter:
- cornea opacity score
- Basis:
- mean
- Time point:
- 48 h
- Score:
- 0.3
- Max. score:
- 1
- Reversibility:
- not reversible
- Remarks on result:
- positive indication of irritation
- Irritation parameter:
- iris score
- Basis:
- mean
- Time point:
- 48 h
- Score:
- 0.2
- Max. score:
- 1
- Reversibility:
- fully reversible
- Remarks on result:
- probability of weak irritation
- Irritation parameter:
- conjunctivae score
- Basis:
- mean
- Time point:
- other: 48 h
- Score:
- 0.5
- Max. score:
- 2
- Reversibility:
- fully reversible within: 8 d (exemption: reddening)
- Remarks on result:
- positive indication of irritation
- Irritant / corrosive response data:
- 24 h after application of the test substance, 2/6 animals showed opacicity of the cornea grade 1 (area 1/4-1/2), 1/6 animal showed circumcorneal injection, 6/6 animals showed redness of the conjunctivae grade 2, 5/6 showed swelling of the conjunctivae grade 1 and 6/6 showed secretion. 48 h after application of the test substance, 2/6 animals showed opacicity of the cornea grade 1 (area 1/4-1/2), 1/6 animal showed circumcorneal injection, 5/6 animals showed redness of the conjunctivae grade 1-2, 4/6 showed swelling of the conjunctivae grade 1 and 4/6 showed secretion. 72 h after application of the test substance, 2/6 animals showed opacicity of the cornea grade 1 (area 1/4-1/2), 1/6 animal showed circumcorneal injection, 5/6 animals showed redness of the conjunctivae grade 1-2, 4/6 showed swelling of the conjunctivae grade 1 and 3/6 showed secretion.
Opacicity of the cornea (grade 1, area 1/4-1/2) persisted in 2/6 animals while conjunctival redness grade 1 persisted in 3/3 animals until the end of the observation period. Other signs of toxicity were not noted. - Interpretation of results:
- Category 2 (irritating to eyes) based on GHS criteria
- Conclusions:
- From the experimental results, it is concluded that sodium 3-nitrobenzenesulphonate is regarded as irritating to the eye of white Vienna rabbits.
- Executive summary:
A Draize Test was carried out for chemical Sodium 3-nitrobenzenesulphonate (CAS no: 127-68-4) to assess the eye irritation potential of six white Vienna rabbits.
About 0.1ml of test chemical was instilled into the eye of each rabbits and the eyes were not washed out after 24 hours after substance application.
Findings were graded as described in OECD test guideline 405 and were recorded after 24, 48, 78 hours, and at the end of the observation period (8 days).
24 h after application of the test substance, 2/6 animals showed opacicity of the cornea grade 1 (area 1/4-1/2), 1/6 animal showed circumcorneal injection, 6/6 animals showed redness of the conjunctivae grade 2, 5/6 showed swelling of the conjunctivae grade 1 and 6/6 showed secretion. 48 h after application of the test substance, 2/6 animals showed opacity of the cornea grade 1 (area 1/4-1/2), 1/6 animal showed circumcorneal injection, 5/6 animals showed redness of the conjunctivae grade 1-2, 4/6 showed swelling of the conjunctivae grade 1 and 4/6 showed secretion. 72 h after application of the test substance, 2/6 animals showed opacity of the cornea grade 1 (area 1/4-1/2), 1/6 animal showed circumcorneal injection, 5/6 animals showed redness of the conjunctivae grade 1-2, 4/6 showed swelling of the conjunctivae grade 1 and 3/6 showed secretion. Opacity of the cornea (grade 1, area 1/4-1/2) persisted in 2/6 animals while conjunctival redness grade 1 persisted in 3/3 animals until the end of the observation period. Other signs of toxicity were not noted.
Thus from the observed findings, it is concluded that the chemical Sodium 3-nitrobenzenesulphonate (CAS no: 127-68-4) was irritating to the eyes of white Vienna rabbits.
Reference
Endpoint conclusion
- Endpoint conclusion:
- adverse effect observed (irritating)
Respiratory irritation
Endpoint conclusion
- Endpoint conclusion:
- no study available
Additional information
Skin irritation:
Various studies has been investigated for the test chemical to observe the potential for dermal irritation to a greater or lesser extent. The studies are based on in vivo experiments in rabbits for target chemical. The predicted data using the Danish QSAR database has also been compared with the experimental data and summarized as below;
A Draize Test was carried out for test chemical on skin of six white Vienna rabbits in accordance with OECD test guideline 404. The chemical was applied to the intact or abraded skin of each rabbit at a dose of 50% of Sodium 3-nitrobenzenesulphonate in water for 24 hours under occlusive condition. After the application time, the skin was washed with water which may have contained a mild detergent. Animals were observed for 8 days and skin changes were observed on working days. Findings were recorded after 24, 72 hours and at the end of the observation period. Findings were graded as described in OECD test guideline 404. The chemical caused slight erythema and edema which were fully reversible within 8 days. Since the observed effects were not persisted during the observed period, the test chemical was considered as not irritating to the skin of white Vienna rabbits.
Another Skin irritation study was conducted by on Vienna White rabbits for test chemical to determine the skin effects caused by the chemical over a period of 8 days. The chemical was applied to the intact or abraded skin of each rabbit at a dose of 50% of Sodium 3-nitrobenzenesulphonate in water under occlusive condition. Skin reactions were evaluated at 5 min and 2 hours and on day 8. The chemical did not produce any sign of skin lesions. Thus rom the experimental results, it is concluded that the chemical was considered to be not irritating to the skin of white Vienna rabbits.
The overall results were further supported by the predicted data estimated by Danish QSAR database. According to Danish QSAR database, the skin irritation effects were estimated by using four different models i.e, Battery, Leadscope, SciQSAR and CASE Ultra for test chemical. Based on estimation, no skin irritation reactions were observed in rabbits. Therefore, the test chemical was considered to be not irritating.
Based on the available data for key and supporting studies, it can be concluded that test chemical is unable to cause skin irritation and considered as not irritating. Comparing the above annotations with the criteria of CLP regulation, it can be classified under the category “Not Classified”.
Eye irritation:
In different studies, the test chemical has been investigated for potential for ocular irritation to a greater or lesser extent. The studies are based on in vivo experiments in rabbits for target chemical that have been summarized as below;
A Draize Test was carried out for test chemical to assess the eye irritation potential of six white Vienna rabbits. About 0.1ml of test chemical was instilled into the eye of each rabbits and the eyes were not washed out after 24 hours after substance application. Findings were graded as described in OECD test guideline 405 and were recorded after 24, 48, 78 hours, and at the end of the observation period (8 days). 24 h after application of the test substance, 2/6 animals showed opacicity of the cornea grade 1 (area 1/4-1/2), 1/6 animal showed circumcorneal injection, 6/6 animals showed redness of the conjunctivae grade 2, 5/6 showed swelling of the conjunctivae grade 1 and 6/6 showed secretion. 48 h after application of the test substance, 2/6 animals showed opacity of the cornea grade 1 (area 1/4-1/2), 1/6 animal showed circumcorneal injection, 5/6 animals showed redness of the conjunctivae grade 1-2, 4/6 showed swelling of the conjunctivae grade 1 and 4/6 showed secretion. 72 h after application of the test substance, 2/6 animals showed opacity of the cornea grade 1 (area 1/4-1/2), 1/6 animal showed circumcorneal injection, 5/6 animals showed redness of the conjunctivae grade 1-2, 4/6 showed swelling of the conjunctivae grade 1 and 3/6 showed secretion. Opacity of the cornea (grade 1, area 1/4-1/2) persisted in 2/6 animals while conjunctival redness grade 1 persisted in 3/3 animals until the end of the observation period. Other signs of toxicity were not noted. Thus from the observed findings, it is concluded that the chemical was irritating to the eyes of white Vienna rabbits.
Another ocular irritation study of test chemical was conducted on rabbits which supported the above mentioned result. In this study, the chemical was installed at a dose of 20 mg for 24 hours into the eye of each rabbits, which produced moderate irritation. Therefore the test chemical was considered to be moderately irritating to the eye of rabbits.
Thus based on the available data for the target as well as supporting studies, it can be concluded that test chemical is able to cause severe eye irritation and considered as irritating. Comparing the above annotations with the criteria of CLP regulation, it can be classified under the category “Category 2”.
Justification for classification or non-classification
The skin and eye irritation potential of test chemical were observed in various studies. The results obtained from these studies indicates that the chemical is unlikely to cause skin irritation but can cause severe eye damage. Hence the test chemical can be classified under the category “Not Classified” for skin and “Category 2” for eye as per CLP.
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