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EC number: 201-081-7 | CAS number: 78-08-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 13 Feb - 17 Feb 2006
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- other: Circular on Test Methods of New Chemical Substances (Japan), Fish acute toxicity test
- Deviations:
- no
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- GLP compliance:
- yes
- Remarks:
- according to the Japanese GLP Standard
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
PHYSICO-CHEMICAL PROPERTIES
- Melting point: < 0°C
- Boiling point: 123°C
- Vapour pressure: < 2.7 kPa at 25 °C
- Water solubility (under test conditions): low
- Stability in water: Test item is hydrolyzed (half-life: within 30 min) - Analytical monitoring:
- yes
- Details on sampling:
- - Sampling method: sampled directly by each vessel and pre-treatment was not performed
- Sample storage conditions before analysis: no strage before analysis
- Methanol concentration was measured, since the test item was quickly hydrolyzed - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: test item was measured and diluted in dilution water by stirring for 120 min - Test organisms (species):
- Oryzias latipes
- Details on test organisms:
- TEST ORGANISM
- Common name: Medaka fish
- Source: Amishima Fishing, Yokohama, Japan
- Age at study initiation (mean and range, SD): within 1 year old
- Length at study initiation (mean and range): 2.19 (2.00 - 2.40) cm
- Weight at study initiation (mean and range): 0.068 (0.053 - 0.082) g
- Feeding during test: no food
ACCLIMATION
- Acclimation period: 19 Jan - 13 Feb 2006
- Acclimation conditions (same as test or not): similar to the test condition (acclimation condition: flow through, test condition: semi-static)
- Type and amount of food: Tetramin, 2% of body weight
- Health during acclimation (any mortality observed): mortaliy was < 5% during 7days before exposure - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
- Hardness:
- 52 mg/L
- Test temperature:
- 23.3 - 24.3 (24 ± 1 °C), measured at0, 24, 48, 72 and 96 hours after exposure.
- pH:
- 6.8 - 7.4, measured at0, 24, 48, 72 and 96 hours after exposure.
- Dissolved oxygen:
- 6.3 - 8.4 mg/L, measured at0, 24, 48, 72 and 96 hours after exposure.
- Nominal and measured concentrations:
- Nominal concentration: 100 mg/L
Measured concentration: 92.2 mg/L (time weighted mean)
The measured concentration was calculated based on the concentration of methanol yielded on hydrolysis - Details on test conditions:
- TEST SYSTEM
- Test vessel: 5 L aquarium
- Type: closed with a teflon sheet
- Fill volume: 5 L
- Aeration: no
- Renewal rate of test solution (frequency/flow rate): every 24 hours
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates):1
- No. of vessels per control (replicates): 1
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Charcoal-treated, dechlorinated, treated with sodium thiosulfateand to get rid of chlor perfectly, aerated tap water in Yokohama, Japan
- Alkalinity: 42 mg/L
- Ca/mg ratio: 14/3.9
- Culture medium different from test medium: same medium
- Intervals of water quality measurement: 6 months
OTHER TEST CONDITIONS
- Adjustment of pH: no adjustment of pH
- Photoperiod: 16 hours in the dark, 8 hours in the light
- Light intensity: < 1000 lux
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
mortality and general condition (abnormal behavior and swim ability) were observed after 24, 48, 72 hours exposure and at the end of the study.
TEST CONCENTRATIONS
- Range finding studies were performed twice.
- Test concentrations: 1) 1, 10 and 100 mg/L, 2) 100 mg/L
- Results used to determine the conditions for the definitive study:
1) No mortality was observed in 1 and 10 mg/L. The mortality in 100 mg/L was 20%. No other toxicity was observed in all group.
2) No toxicity and mortality was observed in 100 mg/L. - Reference substance (positive control):
- yes
- Remarks:
- copper sulfate pentahydrate (analytical grade)
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 92.2 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: Measured concentration is based on concentration of methanol
- Duration:
- 96
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 92.2 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- test mat.
- Remarks on result:
- other: Measured concentration is based on concentration of methanol
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Details on results:
- - Behavioural abnormalities: No toxicological symptom was observed
- Mortality of control: 0%
- Other adverse effects control: No adverse effect in control - Results with reference substance (positive control):
- - Results with reference substance valid? Yes
- LC50: 0.77 mg/L - Sublethal observations / clinical signs:
Table 1. Mesured concentration of methanol
Nominal concentration
(mg/L)
Mean measured concentration, mg/L
(Percent of nominal)
Mean (a) measured
Concentration, mg/L
0 hour
new
24 hours
old
72 hours
new
96 hours
old
Control
< 0.03
< 0.03
< 0.03
< 0.03
64.8 (b)
56.5
(87)
54.6
(84)
67.1 (104)
61.3
(95)
59.8
(92)
(a): time weighted mean
(b): theoretical concentration as methanol
new: freshly prepared test solutions
old: test solutions on 24 hours after preparation of new solutions
Table 2. Caluculated concentration of the test substance using methanol concentration
Nominal concentration
(mg/L)
Mean measured concentration, mg/L
(Percent of nominal)
Mean (a) measured
Concentration, mg/L
0 hour
new
24 hours
old
72 hours
new
96 hours
old
Control
< 0.04
< 0.04
< 0.04
< 0.04
100
87.1
(87)
84
(84)
103 (104)
94.5 (95)
92.2
(92)
(a): time weighted mean
Table 3. Mortality of the Medaka (Oryzias latipes)
Nominal concentration (mg/L)
Mean measured concentration
(mg/L)
Cumulative Number of Dead
(Percent Mortality)
24 Hour
48 Hours
72 Hours
96 Hours
Control
-
0 (0)
0 (0)
0 (0)
0 (0)
100
92.2
0 (0)
0 (0)
0 (0)
0 (0)
- Validity criteria fulfilled:
- yes
- Conclusions:
- The LC50 of 2768-02-7 was measured to be > 92.2 mg/l. Due to the rapid rate of hydrolysis, the measured concentration was based on the hydrolysis product, methanol.
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- 1993-10-11 to 1993-10-15
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Guideline study
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.1 (Acute Toxicity for Fish)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: 100 mg/l
- Concentrations measured in fresh test medium at the start of the test and then again prior to renewal after 24 hours exposure. Concentrations also determined in fresh test media prepared for renewals at 24, 48 and 72 hours. - Vehicle:
- no
- Details on test solutions:
- Limit test at a nominal concentration of 100 mg/l. Test media prepared by dilution of a 10000 mg/l stock solution.
- Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM
- Common name: Zebrafish
- Source: West aquarium, Bad Lauterberg, Germany
- Length at study initiation (length definition, mean, range and SD): 3.0 cm ±0.5 cm
- Feeding during test: none
ACCLIMATION
- Acclimation period: at least 14 days
- Acclimation conditions (same as test or not): yes
- Type and amount of food: TetraMin® at 1% of body weight
- Feeding frequency: Daily
- Health during acclimation (any mortality observed): The fish exhibit normal behaviour and are free from visible signs of disease at the start of the test. - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
- Hardness:
- 10.7 ºdH
- Test temperature:
- 20 ±1ºC
- pH:
- 7.7 - 8.8
- Dissolved oxygen:
- 7.7 - 8.9 mg/l
- Nominal and measured concentrations:
- Nominal: 100 mg/l
Measured concentrations in fresh media at start of test and at 24, 48 and 72 h: 120, 108, 93 and 96 mg/l
Measured concentration in old test media prior to renewal at 24 h: 92 mg/l - Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Type: open aquarium
- Material, size, headspace, fill volume: 20 litre
- Aeration: Continuous
- Renewal rate of test solution (frequency/flow rate): Daily
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Biomass loading rate: 0.4 g/l
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Potable water from Gelsenwasser AG
OTHER TEST CONDITIONS
- Adjustment of pH: None
- Photoperiod: 16 h light/8 h dark
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Mortality after 24, 48, 72 and 96 h - Reference substance (positive control):
- no
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- - Mortality of control: 0
- Validity criteria fulfilled:
- yes
- Conclusions:
- A 96-hour LC50 value of >100 mg/l and NOEC of ≥100 mg/l have been determined for the effects of the test substance on mortality of Brachydanio rerio. It is likely that the test organisms were primarily exposed to the hydrolysis products of the substance.
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- Not reported
- Reliability:
- 4 (not assignable)
- Rationale for reliability incl. deficiencies:
- other: Secondary literature
- Principles of method if other than guideline:
- Not described
- GLP compliance:
- not specified
- Analytical monitoring:
- not specified
- Vehicle:
- no
- Test organisms (species):
- Pimephales promelas
- Test type:
- not specified
- Water media type:
- not specified
- Dose descriptor:
- LC50
- Effect conc.:
- > 2 000 mg/L
- Nominal / measured:
- not specified
- Conc. based on:
- not specified
- Basis for effect:
- mortality (fish)
- Validity criteria fulfilled:
- not specified
- Conclusions:
- An LC50 value of >2000 mg/l has been reported for the effects of the test substance on mortality of Pimephales promelas. The result is reported in secondary literature and its reliability cannot be assessed.
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- Not reported
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Guideline study with acceptable restrictions. No GLP or analysis of exposure media and no description of the test substance.
- GLP compliance:
- no
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- no data
- Test organisms (species):
- Oncorhynchus mykiss (previous name: Salmo gairdneri)
- Details on test organisms:
TEST ORGANISM
- Common name: Rainbow trout
- Source: Fender's Fish Hatchery
- Feeding during test: no data
ACCLIMATION
- Acclimation period: A minimum of two weeks
- Acclimation conditions: same as test
- Type and amount of food: not reported- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Hardness:
- reconstitiuted soft water
- Test temperature:
- 12ºC
- pH:
- no data
- Dissolved oxygen:
- no data
- Salinity:
- Not applicable
- Nominal and measured concentrations:
- Nominal concentrations: 0(Control), 100, 320 and 1000 mg/l
- Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Type: open
- Material: Polyethylene lined
- Aeration: no data
- Renewal rate of test solution (frequency/flow rate): no data
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Reconstituted soft water (EPA 660/3-75-009)
- Culture medium different from test medium: no
- Intervals of water quality measurement: no data
OTHER TEST CONDITIONS
- Adjustment of pH: no data
- Photoperiod: no data
- Light intensity: no data
EFFECT PARAMETERS MEASURED (with observation intervals if applicable): daily mortality
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 3.2
- Range finding study: no data - Reference substance (positive control):
- no
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 191 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: 125-281
- Details on results:
- - Mortality of control: 10% (1 fish)
- Other adverse effects control: none - Reported statistics and error estimates:
- The LC50 value was calculated by probit analysis based on Finney's Method (Statistical Methods in Biological Assay, 1952).
- Sublethal observations / clinical signs:
Table 1. Test results
Nominal test concentration (mg/l) Percentage mortality after 24 hours Percentage mortality after 48 hours Percentage mortality after 72 hours Percentage mortality after 96 hours 0 (Control) 0 0 10 10 100 0 0 0 0 320 30 100 100 100 1000 90 100 100 100 - Validity criteria fulfilled:
- yes
- Conclusions:
- A 96-hour L50 value of 191 mg/l and NOEC of 100 mg/l have been determined for the effects of the test substance on mortality of Oncorhynchus mykiss. It is likely that the test organism were primarily exposed to the hydrolysis product of the test substance.
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- Not reported
- Reliability:
- 4 (not assignable)
- Rationale for reliability incl. deficiencies:
- other: Documentation insufficient for assessment
- Principles of method if other than guideline:
- Not described
- GLP compliance:
- not specified
- Analytical monitoring:
- not specified
- Vehicle:
- not specified
- Test organisms (species):
- Oncorhynchus mykiss (previous name: Salmo gairdneri)
- Test type:
- not specified
- Water media type:
- not specified
- Hardness:
- no data
- Test temperature:
- no data
- pH:
- no data
- Dissolved oxygen:
- no data
- Salinity:
- no data
- Nominal and measured concentrations:
- no data
- Details on test conditions:
- no data
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- not specified
- Conc. based on:
- not specified
- Basis for effect:
- mortality (fish)
- Validity criteria fulfilled:
- not specified
- Conclusions:
- An LC50 value of >100 mg/l has been reported for the effects of the test substance on mortality of Oncorhynchus mykiss. There is insufficient information on the study to assess the reliability of the result.
- Endpoint:
- short-term toxicity to fish
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Justification for type of information:
- Please refer to overall summary (IUCLID Section 6.1) and attached justification for grouping in IUCLID Section 13.
- Reason / purpose for cross-reference:
- read-across source
- Reason / purpose for cross-reference:
- read-across source
- Reason / purpose for cross-reference:
- read-across source
- Reason / purpose for cross-reference:
- read-across source
- Reason / purpose for cross-reference:
- read-across source
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 92.2 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: Measured concentration is based on concentration of methanol
- Remarks:
- MOE, 2006
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 191 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: Dow Corning 1979
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- not specified
- Conc. based on:
- not specified
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: Bemment 2001
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: Hüls AG 1995
- Dose descriptor:
- LC50
- Effect conc.:
- > 2 000 mg/L
- Nominal / measured:
- not specified
- Conc. based on:
- not specified
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: Chandra 1997
Referenceopen allclose all
Description of key information
In a 96 h test no effects were observed at > 92.2 mg/L meas. (TWA) (> 100 mg/l nominal) to Oryzias latipes (OECD 203, RL2)
Key value for chemical safety assessment
Additional information
Measured data were not available for triethoxy(vinyl)silane (CAS 78-08-0), therefore read across of data from the analogue substance trimethoxyvinylsilane (CAS 2768-02 -7) was deemed acceptable for assessment.
A limit test (OECD Guideline 203 Fish Acute Toxicity Test, Ministry of the Environment, Government of Japan, 2006) was conducted with a nominal test concentration of 100 mg/L corresponding to a 92 mg/L mean measured (based on analytical verification of the hydrolysis product methanol). No toxic effects were observed up to this concentration to the tested organism Oryzias latipes.
Several other studies verify this result. In the supporting study of Dow Corning, a 96 h LC50 value of 191 mg/l and a NOEC of 100 mg/l for the effects of the test substance on mortality of Oncorhynchus mykiss (1979) were determined. For the same test species an LC50 > 100 mg/l was calculated (Momentive, 2001). In a GLP study according to EU-Method C.1 (Hüls AG, 1995), the 96 h LC50 for Brachydanio rerio was found to be > 100 mg/l and the NOEC was ≥ 100 mg/l. In a secondary literature of Chandra (1997) the LC50 for Pimephales promelas was > 2000 mg/l.
As the substance is subject to rapid hydrolysis, it is very likely that the test organisms were primarily exposed to hydrolysis products retained in the test media.
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