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Diss Factsheets
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EC number: 246-910-3 | CAS number: 25376-45-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to reproduction: other studies
Administrative data
- Endpoint:
- toxicity to reproduction: other studies
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Research study not equivalent or similar to OECD guideline. Useful data for evaluation.
Data source
Reference
- Reference Type:
- publication
- Title:
- Unnamed
- Year:
- 2 000
Materials and methods
Test guideline
- Qualifier:
- no guideline available
- Principles of method if other than guideline:
- In a compilation of 517 chemicals, that had been screened for estrogenic activity in vitro using a yeast two-hybrid assay, also 2,4-TDA is listed. Whereas 64 out of the 517 tested compounds were evaluated as positive, for 2,4-TDA no estrogenic activity was revealed.
- GLP compliance:
- not specified
- Type of method:
- in vitro
Test material
- Reference substance name:
- 4-methyl-m-phenylenediamine
- EC Number:
- 202-453-1
- EC Name:
- 4-methyl-m-phenylenediamine
- Cas Number:
- 95-80-7
- Molecular formula:
- C7H10N2
- IUPAC Name:
- 4-methylbenzene-1,3-diamine
- Details on test material:
- No data.
Constituent 1
Test animals
- Species:
- other: Yeast cells (Saccharomyces cerevisiae Y190)
- Details on test animals or test system and environmental conditions:
- Not applicable.
Administration / exposure
- Route of administration:
- other: in vitro
- Vehicle:
- other: DMSO or water
- Details on exposure:
- The yeast two-hybrid system based on the ligand-dependent interaction of nuclear hormone receptors with coactivators.
- Analytical verification of doses or concentrations:
- not specified
- Details on analytical verification of doses or concentrations:
- No data.
- Duration of treatment / exposure:
- 4 hours
- Frequency of treatment:
- Once
- Duration of test:
- Over 4 hours
Doses / concentrations
- Remarks:
- Doses / Concentrations:
2.5 ul of 2,4-TDA
Basis:
no data
- No. of animals per sex per dose:
- Not applicable.
- Details on study design:
- The yeast two-hybrid assay system was used with the estrogen receptor, ERα, and the coactivator, TIF2. Two expression plasmids, pGBT9-ERLBD and pGAAD424-TIF-2, introduced into yeast cells (Saccharomyces cerevisiae Y190), which carry a β-galactosidase reporter gene and require tryptophan and leucine for growth. The cells were preincubated overnight at 30°C in SD medium free from tryptophan and leucine. The culture (250 μl) in a small test tube was then mixed with a DMSO solution (2.5 μl) of test chemical and incubated for 4 h at 30°C. After washing by centrifugation, the cells were digested enzymatically by incubation with 1 mg/ml Zymolyase 20T (200 μl) at 30°C for 15 min. The lysate was mixed with 4 mg/ml ONPG (40 μl)
and reacted until development of a yellow color (usually 30 min) before the reaction was stopped by the addition of 1 M Na2CO3 (100 μl). An aliquot (150 μl) was taken into each of 96 wells of a microplate. Absorbances at 420 and 550 nm were read on a microplate reader to estimate estrogenic activity.
The results were evaluated by relative activity, expressed as REC10 (10% relative effective concentration), that is the concentration of 2,4-TDA showing 10% of the agonist activity of 10–7 M E2, which is the optimum concentration for E2. When the activity of the test substance was higher than REC10 within the concentration tested, we judged the chemical as positive. When it was judged to be negative, we indicated the highest dose we tested. - Statistics:
- No data
Results and discussion
Observed effects
Applicant's summary and conclusion
- Conclusions:
- In a compilation of 517 chemicals, that had been screened for estrogenic activity in vitro using a yeast two-hybrid assay, also 2,4-TDA is listed (Nishihara et al., 2000). Whereas 64 out of the 517 tested compounds were evaluated as positive, for 2,4-TDA no estrogenic activity was revealed.
- Executive summary:
In this yeast two-hybrid assay, 2,4 -TDA showed no estrogenic activity.
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