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EC number: 219-433-3 | CAS number: 2436-90-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Eye irritation
Administrative data
- Endpoint:
- eye irritation: in vitro / ex vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From 27 July 2010 to 17 September 2010
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Study well conducted and in compliance with GLP
Cross-referenceopen allclose all
- Reason / purpose for cross-reference:
- reference to same study
- Reason / purpose for cross-reference:
- reference to other study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 010
- Report date:
- 2010
Materials and methods
Test guideline
- Qualifier:
- no guideline available
- Principles of method if other than guideline:
- Evaluation of the primary ocular irritation of Dimethyloctadiene after application on a human reconstituted corneal epithelium model and quantification of cellular viability by reduction test of MTT (Mosmann, T. 1983) and T50 determination, exposure time that causes 50% of mortality. This reconstituted corneal epithelium model could be consider as the most similar to the human corneal because it is a 3D model.
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- 3,7-dimethylocta-1,6-diene
- EC Number:
- 219-433-3
- EC Name:
- 3,7-dimethylocta-1,6-diene
- Cas Number:
- 2436-90-0
- Molecular formula:
- C10H18
- IUPAC Name:
- 3,7-dimethylocta-1,6-diene
- Details on test material:
- - Name of test material (as cited in study report): dimethyloctadiene
- Physical state: colourless translucent liquid
- Analytical purity: 91.1 %
- Impurities (identity and concentrations): 1-Isopropenyl-2,3-dimethyl-cyclopentane
- Purity test date: 31 March 2010
- Lot/batch No.: EC205R
- Expiration date of the lot/batch: 18 March 2011
- Storage condition of test material: kept in refrigerator under nitrogen flow, protected from light and humidity
Constituent 1
Test animals / tissue source
- Species:
- human
- Strain:
- not specified
Test system
- Vehicle:
- unchanged (no vehicle)
- Remarks:
- dimethyloctadiene is tested at 100% (undiluted sample).
- Controls:
- other: negative control= untreated epithelium (epithelium in maintenance medium); positive control= Sodium Dodecyl Sulfate (CAS 151-21-3) at 0.5% (W/W) in sterile water
- Amount / concentration applied:
- TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 30 µL
- Concentration (if solution): the test item is applied as supplied (ie. undiluted)
VEHICLE: not applicable - Duration of treatment / exposure:
- test item: 10 min, 60 and 180 min
Positive control: 10 and 60 min
Negative control: 180 min - Observation period (in vivo):
- at the end of each incubation, the MTT test was performed immediately.
- Number of animals or in vitro replicates:
- all conditions (test item, negative control and positive control) were performed in duplicate
- Details on study design:
- REMOVAL OF TEST SUBSTANCE
- Washing (if done): at the end of each incubation period rinsing of the epithelia under a jet of PBS (using a washing bottle), by possibly removing the excess product adhering to epithelium using a cotton-wool bud.
- Time after start of exposure: at the end of exposure time (10, 60, or 180 min)
SCORING SYSTEM: not applicable
TOOL USED TO ASSESS SCORE: not applicable
Results and discussion
In vitro
Results
- Irritation parameter:
- other: T50
- Value:
- >= 180
Any other information on results incl. tables
None
Applicant's summary and conclusion
- Interpretation of results:
- not irritating
- Remarks:
- Migrated information Criteria used for interpretation of results: EU
- Conclusions:
- Under the test conditions, Dimethyloctadiene is not classified as irritating to eyes.
- Executive summary:
In an in vitro ocular primary irritation test, performed in compliance with GLP, human reconstructed corneal epithelium was treated with undiluted Dimethyloctadiene. The test substance was in contact with corneal epithelium for 10, 60 or 180 minutes and then the epithelia were rinsed.
MTT conversion assay was peformed to evaluate the percentage of cellular viability of the epithelium in order to caculate the T50, which is the time when 50% of the cells are dead.
Negative control (maintenance medium) and positive control (Sodium dodecyl sulfate) were also used and the results were acceptable for these controls.
The T50 for Dimethyloctadiene calculated from 3 contact time points was higher than 180 minutes. Therefore, under the test conditions, Dimethyloctadiene is not classified as irritating to eyes.
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