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Diss Factsheets
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EC number: 246-836-1 | CAS number: 25321-14-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to soil microorganisms
Administrative data
- Endpoint:
- toxicity to soil microorganisms
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: test procedure in accordance with national standard methods
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 995
- Report date:
- 1993
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: BBA Nr. VI, 1-1 (1990): Dehydrogenase activity of soil microflora
- Principles of method if other than guideline:
- Method developed by "Biologische Bundesanstalt, Braunschweig (Germany)"
- GLP compliance:
- not specified
Test material
- Reference substance name:
- 2,4-dinitrotoluene
- EC Number:
- 204-450-0
- EC Name:
- 2,4-dinitrotoluene
- Cas Number:
- 121-14-2
- Molecular formula:
- C7H6N2O4
- IUPAC Name:
- 1-methyl-2,4-dinitrobenzene
- Details on test material:
- - Name of test material (as cited in study report): 2,4-Dinitrotoluene (2,4-DNT)
- Analytical purity: 99%
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test substrate
- Vehicle:
- not specified
Test organisms
- Test organisms (inoculum):
- soil
Study design
- Total exposure duration:
- 28 d
Test conditions
- Test temperature:
- 22°C
- Moisture:
- ca. 60% of maximum field capacity
- Details on test conditions:
- - natural soil microorganisms were tested;
- two soils were used ( sandy soil: pH 6.8; loamy soil: pH 7.3-7.4);
- incubation in the dark - Nominal and measured concentrations:
- 5 and 50 mg/kg soil dw
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 28 d
- Dose descriptor:
- other: EC13
- Effect conc.:
- 5 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: dehydrogenase activity
- Remarks on result:
- other: loamy soil
- Duration:
- 28 d
- Dose descriptor:
- other: EC43
- Effect conc.:
- 50 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: dehydrogenase activity
- Remarks on result:
- other: loamy soil
Any other information on results incl. tables
Reduction of dehydrogenase activity of
a. sandy soil microorganisms: 26-46% during the whole
exposure period and at both concentrations tested;
b. loamy soil microorganisms: at the beginning of the test,
no effect was observed with a concentration of 5 mg/kg,
whereas a concentration of 50 mg/kg caused an inhibition of
32%;
inhibition was highest at the 14th day of exposure at both
concentrations tested (60-70%); at the end of the test,
inhibition had declined to 13 and 43% at 2,4-DNT
concentrations of 5 and 50 mg/kg, respectively.
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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