Registration Dossier
Registration Dossier
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 241-774-1 | CAS number: 17796-82-6
Ecotoxicological information
Short-term toxicity to fish
Currently viewing:
Administrative data
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 008
- Report date:
- 2008
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.1 (Acute Toxicity for Fish)
- GLP compliance:
- yes
Test material
- Reference substance name:
- N-(cyclohexylthio)phthalimide
- EC Number:
- 241-774-1
- EC Name:
- N-(cyclohexylthio)phthalimide
- Cas Number:
- 17796-82-6
- Molecular formula:
- C14H15NO2S
- IUPAC Name:
- N-(cyclohexylthio)phthalimide
- Details on test material:
- Batch No.:? 093/06
purity: 99.43% w/w
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- Sampling method: mass concentration method, prepared and delivered by Ecotoxicity Group, analysis of samples immediately after delivery
- Sample storage conditions before analysis: at room temperature
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: stirring 2 h + ultrasonic 15 min + filtration
Ca. 30% od Duslin P decomposes during 2 hours.
Test organisms
- Test organisms (species):
- Poecilia reticulata
- Details on test organisms:
- TEST ORGANISM
- Source:Bio Test s.r.o. Konárovice Czech republic
- Age at study initiation (mean and range, SD): 3-4 months old
- Length at study initiation (length definition, mean, range and SD): 2.0 - 3.0 cm
- Weight at study initiation (mean and range, SD): preliminary test - number of fish in beaker: 7, total weight 0.4 -0.7g
full semistatic test- number of fish in beaker: 10 total weight: 1.1-1.3g
- Feeding during test: no food
- without aeration
ACCLIMATION
- Acclimation period: 7 days
- Acclimation conditions (same as test or not): the fish were kept in all-glass aquaria in aerated drinking water
- Type and amount of food: no fed during 72 hours before teh test
- Health during acclimation (any mortality observed): no medical treatment
Study design
- Test type:
- semi-static
- Water media type:
- freshwater
- Total exposure duration:
- 96 h
Test conditions
- Hardness:
- content of sum Ca+Mg ions in dilution water is 2.5 mmol/L
- Test temperature:
- 22+/-2°C with variation of +/- 1°C
- pH:
- dilution water ph=7.8+/-0.2
- Dissolved oxygen:
- preliminary test: stock solution : 8.8 mg/L at 21.2°C
full semi-static test: test solution1: 8.3 mg/L at 21.8°C
2: 8.3 mg/L at 21.2°C
3: 8.7 mg/L at 21.2°C
4: 8.9 mg/L at 21.6°C - Nominal and measured concentrations:
- preliminary test nominal concentration range: 10-1000 mL/L
full semi-static test nominal concentration range : 100-700 mL/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: all-glass aquarium
- Type (delete if not applicable): open / closed
- Material, size, headspace, fill volume:
- Aeration:
- Type of flow-through (e.g. peristaltic or proportional diluter):
- Renewal rate of test solution (frequency/flow rate):
- No. of organisms per vessel: 7 for preliminary test, 10 for semi-static test
- No. of vessels per concentration (replicates):1
- No. of vessels per control (replicates):1
- No. of vessels per vehicle control (replicates):0
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water:prepared just before test by admixing 2.5 mL of stored solution A,B,C and D in 1000mL of distilled water
A= 117.6g CaCl2.2H2O in 1L of distilled water
B= 49.3 g MgSO4.7H2O in 1L of distilled water
C= 25.9 g NaHCO3 in 1L of distilled water
D. 2.3 g KCl in 1L of distilled water
destilled water:
conductivity: 0.165mS/m
pH: 7.6
Oxygen content: 8.8 mg/L at 21.2°C
content of Ca+MG ions is 2.64 mmol/L
- Intervals of water quality measurement: 24 hours
OTHER TEST CONDITIONS
- Photoperiod: daylight
TEST CONCENTRATIONS
- Test concentrations: nominal: 100-700 ml/L - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 0.69 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- dissolved
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: 95%CL
- Duration:
- 24 h
- Dose descriptor:
- LC50
- Effect conc.:
- 1.03 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- dissolved
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: 95%CL
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- 0.83 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- dissolved
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: 95% CL
- Duration:
- 72 h
- Dose descriptor:
- LC50
- Effect conc.:
- 0.83 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- dissolved
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: 95%CL
- Duration:
- 24 h
- Dose descriptor:
- LC100
- Effect conc.:
- 1.44 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- dissolved
- Basis for effect:
- mortality (fish)
- Duration:
- 48 h
- Dose descriptor:
- LC100
- Effect conc.:
- 1.15 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- dissolved
- Basis for effect:
- mortality (fish)
- Duration:
- 72 h
- Dose descriptor:
- LC100
- Effect conc.:
- 1.15 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- dissolved
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC100
- Effect conc.:
- 1.15 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- dissolved
- Basis for effect:
- mortality (fish)
- Duration:
- 24 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.69 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- dissolved
- Basis for effect:
- mortality (fish)
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.39 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- dissolved
- Basis for effect:
- mortality (fish)
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.39 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- dissolved
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.39 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- dissolved
- Basis for effect:
- mortality (fish)
- Results with reference substance (positive control):
- - Results with reference substance valid? YES
- Mortality: meets criteria determined by the interlaboratory test
- LC50: 48h-LC50=143 mg/L
96h- LC50= 137 mg/L
- Other: interlaboratory test 96h-LC50= 96-154 mg/L in 2006
Any other information on results incl. tables
- Sublethal observations / clinical signs:
full semi-static test - Fish mortality
determined conc. TWM mg/L 1.44 0.15 0.69 0.52 0.39 0.31 0.23 C Saturated solution dosing mL/L 700 500 360 260 190 140 100 C Number of live fish 2 h 0 10 10 10 10 10 10 10 mortality % 2 h 100 0 0 0 0 0 0 0 Number of live fish 24 h 0 2 10 10 10 10 10 10 mortality % 24 h 100 80 0 0 0 0 0 0 Number of live fish 48 h 0 0 7 8 10 10 10 10 mortality % 48 h 100 100 30 20 0 0 0 0 Number of live fish 72 h 0 0 7 8 10 10 10 10 mortality % 72 h 100 100 30 20 0 0 0 0 Number of live fish 96 h 0 0 5 8 10 10 10 10 mortality % 96 h 100 100 50 20 0 0 0 0
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Remarks:
- yes for points 1,2,3, not for point 4
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
