Exo-1,7,7-trimethylbicyclo[2.2.1]hept-2-yl acrylate

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1974

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Remarks:

Study conducted pre-GLP.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1974

Report date:

1974

Materials and methods

Principles of method if other than guideline:

Standard acute method. Study conducted pre-GLP.

GLP compliance:

no

Remarks:

study conducted pre-GLP

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Exo-1,7,7-trimethylbicyclo[2.2.1]hept-2-yl acrylate

EC Number:

227-561-6

EC Name:

Exo-1,7,7-trimethylbicyclo[2.2.1]hept-2-yl acrylate

Cas Number:

5888-33-5

Molecular formula:

C13H20O2

IUPAC Name:

(1S,2S,4S)-1,7,7-trimethylbicyclo[2.2.1]heptan-2-yl prop-2-enoate

Test material form:

liquid

Test animals

Species:

rat

Strain:

Wistar

Sex:

male

Details on test animals or test system and environmental conditions:

Seventy male Wistar albino rats weighing between 200 and 300 g were used. The animals were fed, housed and watered in accordance with standard laboratory procedures.

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

unchanged (no vehicle)

Details on oral exposure:

The test animals were fasted for a period of app. 24 hrs prior to testing. The test material was administered as a single dosage.

Doses:

Control = 0, 3000, 3500, 4000, 4500, 5000, 6000 mg/kg

No. of animals per sex per dose:

Total number of animals: 70
10 anmals per dose

Control animals:

yes

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: for mortality and toxicity immeldiately after dosing: 1, 3, 6, 24, 48, and 72 hours post-dosing; and daily thereafter for a total of 14 days
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs

Results and discussion

Effect levels

Effect levels 1

Sex:

male

Dose descriptor:

LD50

Effect level:

5 750 mg/kg bw

Based on:

test mat.

Remarks on result:

other: 24 hrs

Effect levels 2

Key result

Sex:

male

Dose descriptor:

LD50

Effect level:

4 350 mg/kg bw

Based on:

test mat.

Remarks on result:

other: 14 d

Mortality:

please see attached table

Clinical signs:

other: Depression, burrowing, twitching, staggering, abnormal gait, straub tail, clonic convulsions, tremors, respiratory depression, piloerection, and rates of death. Details see attached table.

Applicant's summary and conclusion

Interpretation of results:

not classified

Remarks:

Migrated information EU 1272/2008 Criteria used for interpretation of results: EU

Conclusions:

According to the test result: LD50(14days): 4350 mg/kg bw (male rats) the test substance Isobornyl acrylate has not to be classified with respect to acute oral toxicity (EU GHS criteria; 1272/2008/EU).

Executive summary:

In an acute oral toxicity study according to standard acute method conducted pre-GLP, groups of fasted male Wistar rats obtained a single oral dose of Isobornyl acrylate at a doses up to 6000 mg/kg and were observed for 14 days.

 

Oral LD₅₀= 4350 mg/kg bw

   

Isobornyl acrylate has a low acute toxic potential in rats (EU GHS: no category) based on this test.