Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 203-584-7 | CAS number: 108-45-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study with acceptable restrictions
- Remarks:
- The purity of the test substance was reported to be as received from Akzo Nobel Fibres, Amhem.
- Justification for type of information:
- Additional documentation, provided within the IUCLID Assessment Reports (Section 13), supports the read-across approach.
- Reason / purpose for cross-reference:
- read-across: supporting information
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- yes
- Remarks:
- The test was performed according to a slightly modified OECD test guideline.
- GLP compliance:
- not specified
- Analytical monitoring:
- no
- Vehicle:
- yes
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION:
- Method: The test substance was added to the incubation vessels using a stock solution of 1.0 g/L.
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): Deionised water containing no more than 0.01 mg Cu/L and 1 mg/L non-purgeable organic carbon - Test organisms (species):
- activated sludge of a predominantly industrial sewage
- Details on inoculum:
- - Laboratory culture: Secondary activated sludge was obtained from the WWTP Kleefse Waard in Arnhem. The WWTP Kleefse Waard is an activated sludge plant treating industrial waste water of the site Kleefse Waard.
- Preparation of inoculum for exposure: the activated sludge was diluted 5 times in the test. The influent used as substrate and effluent used to dilute the sludge were also obtained from the WWTP Kleefse Waard - Test type:
- not specified
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 30 min
- Test temperature:
- 20°C
- pH:
- ranged from 8.7-8.9
- Nominal and measured concentrations:
- Nominal: 0, 3.1, 6.3, 12.5, 25, 50, 100 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 200 mL Erlenmeyers
- Other: Homogenized activated sludge was incubated in a shaking water bath (100 Hz, 20°C) for 30 minutes.
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2 - Reference substance (positive control):
- no
- Duration:
- 30 min
- Dose descriptor:
- other: 30% reduction in respiration rate
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Remarks on result:
- other: EC50 could not be calculated
- Details on results:
- The validity of the test is shown by the control respiration rates which are within 16% of each other. The respiration rates and inhibition percentages calculated are in Table 1. At 100 mg/L, a 30% reduction of the respiration rate was observed and EC50 values could therefore not be calculated.
- Validity criteria fulfilled:
- yes
- Conclusions:
- At 100 mg test substance/L, a 30% reduction of the respiration rate was observed and EC50 values could therefore not be calculated. The test substance will not have any adverse effects on the WWTP Kleefe Waard at concentration <10 mg/L.
- Executive summary:
The toxicity of the test substance to activated sludge of the WWTP Kleefse Waard was determined by measuring the respiration rate after a contact time of 30 minutes at various concentrations of the test substance. The inhibitory effect of the test substance at a particular concentration was expressed as a percentage of the two controls. At 100 mg test substance/L, a 30% reduction of the respiration rate was observed and EC50 values could therefore not be calculated. The results demonstrate that the test substance will not have any adverse effects on the WWTP Kleefe Waard at concentration <10 mg/L.
Reference
Respiration Rates, Inhibition Percentages, pH
Concentration (mg/L) |
Activity (mg O2/min.litre) |
Inhibition (%) |
pH |
Control |
0.31 |
- |
8.7 |
Control |
0.26 |
- |
8.7 |
3.1 |
0.31 |
0 |
8.7 |
6.3 |
0.28 |
2 |
8.7 |
12.5 |
0.29 |
0 |
8.7 |
25 |
0.27 |
5 |
8.7 |
50 |
0.28 |
2 |
8.8 |
100 |
0.20 |
30 |
8.9 |
Description of key information
Key value for chemical safety assessment
Additional information
Microbiological respiration inhibition data is provided on p-phenylenediamine in activated sludge of predominantly industrial sewage. A 30% reduction in respiration rate (30 min): 100 mg/L (nominal) based on respiration rate was observed. Additional supporting data is provided for o-phenylenediamine, a structurally related analogue, in activated sludge for predominantly domestic sewage. The EC10 (3 h) and EC50 (3 h) values were 100 mg/L and 580 mg/L respectively. Data from the study with p-phenylenediamine are consistent with the results from the study with o-phenylenediamine.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.