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EC number: 225-625-8 | CAS number: 4979-32-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to soil microorganisms
Administrative data
- Endpoint:
- toxicity to soil microorganisms
- Type of information:
- experimental study planned
- Justification for type of information:
- TESTING PROPOSAL ON SOIL MICROORGANISMS: OECD 216
NON-CONFIDENTIAL NAME OF SUBSTANCE:
- Name of the substance on which testing is proposed to be carried out: N,N- dicyclohexylbenzo-thiazole-2-sulphenamide. The following public name is used: DCBS
- Name of the substance for which the testing proposal will be used [if different from tested substance]: This substance itself only.
CONSIDERATIONS THAT THE GENERAL ADAPTATION POSSIBILITIES OF ANNEX XI OF THE REACH REGULATION ARE NOT ADEQUATE TO GENERATE THE NECESSARY INFORMATION:
- Available GLP studies: There are no GLP studies available for this substance covering the endpoint of terrestrial toxicity on microorganisms.
- Available non-GLP studies: No further studies on terrestrial toxicity on microorganisms are available for this substance.
- (Q)SAR: There are no QSAR models available for this higher tier ecotoxicological endpoint that are sufficiently validated and acceptable (according to OECD Q/SAR validation criteria).
- Weight of evidence & Grouping and read-across: The endpoint terrestrial toxicity to soil microorganisms is currently covered by studies from substances within the sulfenamide group (N-tert-butylbenzothiazole-2-sulphenamide, public name: TBBS and N-cyclohexylbenzothiazole-2-sulfenamide, public name: CBS): The test on microbial nitrate formation resulted in a NOEC of 205 mg/kg soil dw for TBBS and of 128 mg/kg soil dw for CBS after 28 days. Additionally, information on the other trophic levels were obtained by studies according to TG OECD 222 and OECD 208. For E. fetida NOECs of 133.4 mg/kg soil dw for TBBS and ≥500 mg/kg soil dw for CBS were determined after a test period of 56 d. For the toxicity of TBBS to terrestrial plants, the lowest NOEC of 3.16 mg/kg soil dry weight (dw) was obtained after 16 d by a conservative evaluation of the diverging results of two test runs with P. sativum. For CBS the lowest NOEC of 31.6 mg/kg soil dw was determined for A. cepa, B. rapa, P. sativum and S. lycopersium. Hence, terrestrial plants are identified as the most sensitive species for soil organisms for the sulfenamide group.
However, TBBS, CBS and DCBS differ significantly in their adsorptive properties as indicated by logKow (TBBS) = 3.36 (exp.); logKoc (TBBS) = 2.7 (calc.); logKow (CBS) = 5.02 (exp.); logKoc (CBS) = 3.56 (calc.) and logKow(DCBS) = 5.95 (calc), logKoc(DCBS) = 4.7 (calc.). Consequently, the water solubility for DCBS with a measured value of 1.9 µg/L is lower than the water solubility of both TBBS (1.74 mg/L) and CBS (0.32 mg/L). The substance is considered not readily biodegradable, it has a high potential to adsorb to soil as indicated from its physico-chemical properties and terrestrial plants have been indicated to be the most sensitive species in soil from tests with TBBS and CBS. Hence, DCBS is proposed to be even more adsorptive with a potentially higher toxicity towards the soil compartment. Thus, the registrant intends to elucidate the effect of DCBS on terrestrial microorganisms.
This assessment is supported by section 9.4, column two in annex IX: ‘In particular for substances that have a high potential to adsorb to soil or that are very persistent, the registrant shall consider long-term toxicity testing instead of short-term.’
Overall, the registrant has carefully considered all general adaptation procedures as listed in Annex XI of the REACH regulation. The registrant therefore proposes to conduct an OECD TG 216 study to comply with the standard requirements for the present tonnage band (>1.000 tpa) according REACH regulation annex X 9.4.6 taking the category approach into account.
CONSIDERATIONS THAT THE SPECIFIC ADAPTATION POSSIBILITIES OF ANNEXES VI TO X (AND COLUMN 2 THEREOF) OF THE REACH REGULATION ARE NOT ADEQUATE TO GENERATE THE NECESSARY INFORMATION:
- The registrant has carefully considered the available adaptation possibilities applicable for the discussed endpoint. Adaptation according to REACh Annex IX 9.4 column 2 is not proposed as the exposure assessment indicates that emissions to the environment cannot be excluded.
FURTHER INFORMATION ON TESTING PROPOSAL IN ADDITION TO INFORMATION PROVIDED IN THE MATERIALS AND METHODS SECTION:
- The registrant intends to perform the toxicity to terrestrial microorganisms according to OECD TG 216. According to the guideline, the study design of this study will fit to the physico-chemical properties of the test substance, as this study can deal with substances with low water solubility.
Data source
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 216 (Soil Microorganisms: Nitrogen Transformation Test)
- Deviations:
- no
Test material
- Reference substance name:
- N,N-dicyclohexylbenzothiazole-2-sulphenamide
- EC Number:
- 225-625-8
- EC Name:
- N,N-dicyclohexylbenzothiazole-2-sulphenamide
- Cas Number:
- 4979-32-2
- Molecular formula:
- C19H26N2S2
- IUPAC Name:
- N-(1,3-benzothiazol-2-ylsulfanyl)-N-cyclohexylcyclohexanamine
Constituent 1
Results and discussion
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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