Dimethyl sulphate

Sensitisation data (human)

Administrative data

Endpoint:

sensitisation data (humans)

Type of information:

other: case report

Adequacy of study:

supporting study

Reliability:

4 (not assignable)

Data source

Reference

Reference Type:

publication

Title:

Occupational allergic contact dermatitis due to dimethyl sulfate following sensitization from a severe acute irritant reaction to the reagent

Author:

Yagami, A.

Year:

2009

Bibliographic source:

Contact Derm. 60, 183-184

Materials and methods

Type of sensitisation studied:

skin

Study type:

case report

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

After an accident with DMS and the assumption that allergic contact dermatitis to DMS occured a patch test using DMS dilution series (0.1% pet to 0.0001% pet) in white pet was performed.

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Dimethyl sulphate

EC Number:

201-058-1

EC Name:

Dimethyl sulphate

Cas Number:

77-78-1

Molecular formula:

C2H6O4S

IUPAC Name:

dimethyl sulfate

Details on test material:

- Name of test material (as cited in study report): Dimethyl sulphate
- Physical state: liquid
- no further significant data stated

Method

Type of population:

occupational

Ethical approval:

not specified

Subjects:

- Number of subjects exposed: 1
- Sex: male
- Age: no data
- Race: human, japanese

Clinical history:

for details see "details on study design"

Controls:

no controls

Route of administration:

dermal

Details on study design:

Human case study with additional patch testing:
A 26 -year old Japanese man spilled 100% DMS on his clothing at work. He changed out of the affected clothing 30 min after the accident but did not change his underwear. Our later, blister, redness and swelling were noticed in his genital area. In addition skin eruptions were evident on his right elbow. His symptoms improved with treatment, but new itchy eruptions appeared on his face and extremities on day 8 and disappeared on day 18. On work day 1, he was exposed to DMS again (shoes were not cleaned). Several hours later, itchy erythema recurred at the sites previously affected by the acute irritant reaction. A skin biopsy from the left thigh showed epidermal hyperplasia, sponginess, and a dense perivascular cell infiltrate from the shallow to deep layers of the dermis to assess if occurred a patch test was performed (1 to 0.0001%, each with the patient and two controls). One week later, there was a positive skin reaction to 1% pet and no reaction to 0.01% pet observed at the skin of the patient. In the controls at 1% pet a skin reaction was observed but not at the skin of the control exposed to the 0.1% pet.

Results and discussion

Results of examinations:

One week after exposure, there was a positive skin reaction to 1% pet and no reaction to 0.01% pet observed at the skin of the patient. In the controls at 1% pet a skin reaction was observed but not at the skin of the control exposed to the 0.1% pet.

Applicant's summary and conclusion

Conclusions:

On basis of the results, it can be stated that the patient has an allergic contact dermatitis to DMS and concluded that a suitable concentration of DMS for patch testing was 0.1% pet.