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EC number: 251-646-7 | CAS number: 33703-08-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in mammalian cells
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study with acceptable restrictions
Data source
Reference
- Reference Type:
- publication
- Title:
- An Evaluation of the Genotoxic Potential of Di-lsononyl Adipate
- Author:
- McKee
- Year:
- 1 986
- Bibliographic source:
- Environ Mutagen 8:817-827
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 476 (In Vitro Mammalian Cell Gene Mutation Test)
- GLP compliance:
- no
- Type of assay:
- mammalian cell gene mutation assay
Test material
- Reference substance name:
- Diisononyl adipate
- EC Number:
- 251-646-7
- EC Name:
- Diisononyl adipate
- Cas Number:
- 33703-08-1
- Molecular formula:
- C24H46O4
- IUPAC Name:
- 1,6-bis(7-methyloctyl) hexanedioate
- Test material form:
- liquid
Constituent 1
- Specific details on test material used for the study:
- - Name of test material (as cited in study report): DINA, Cas-No. 33703-08-1
- Analytical purity: 99 %
Method
- Target gene:
- thymidine kinase
Species / strain
- Species / strain / cell type:
- mouse lymphoma L5178Y cells
- Additional strain / cell type characteristics:
- not applicable
- Metabolic activation:
- with and without
- Metabolic activation system:
- Aroclor 1254-induced S9-mix
- Test concentrations with justification for top dose:
- without S9-mix: 0, 7.5, 10, 13, 18, 24, 56, 75, 100 µl/mL
with S9-mix: 0, 5.6, 10, 13, 18, 24, 32, 42, 56, 75, 100 µl/mL - Vehicle / solvent:
- - Vehicle(s)/solvent(s) used: acetone
- Justification for choice of solvent/vehicle: due to solubility
Controls
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- Remarks:
- Acetone
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- 7,12-dimethylbenzanthracene
- ethylmethanesulphonate
- Details on test system and experimental conditions:
- The mouse lymphoma gene mutation assay was conducted essentially as described by Clive and co-workers [1973, 1979; Clive and Spector, 1975]. Suspension cultures of mouse lymphoma cells, heterozygous for thymidine kinase activity, were grown in Fisher medium for leukemic mouse cells supplemented with 0.1% pluronic and 10% heat-inactivated horse serum (Fl0P) and were exposed to test substances in the same medium. Treated cells were grown in Fl0P for 48 hr to allow mutation expression. Approximately 3 x l0^6 cells from each culture were then plated in a selective medium containing 3 µg/ml trifluorothymidine (TFT) to select for mutant clones. Appropriately diluted cells from each culture were also seeded in plates without TFT for viability determinations (200 cells/plate). Mutant and total colony counts at each dose point were determined by triplicate plating. Colonies were counted with an automatic colony counter; differential sizing was not performed.
- Evaluation criteria:
- The validity of the experimental assay was verified through the use of concurrent positive, control materials, 7,12dimethyl-benzanthracene (DMBA) and ethylmethane sulfonate (EMS). Increased mutation frequencies induced by the positive control materials under appropriate conditions demonstrated both the integrity of the metabolic activation system and the responsiveness of the assay system as compared to its historical performance in the laboratory.
Results and discussion
Test results
- Species / strain:
- mouse lymphoma L5178Y cells
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not specified
- Positive controls validity:
- valid
- Additional information on results:
- RANGE-FINDING/SCREENING STUDIES: no data
COMPARISON WITH HISTORICAL CONTROL DATA: yes
ADDITIONAL INFORMATION ON CYTOTOXICITY:
Applicant's summary and conclusion
- Conclusions:
- The provided mammalian cell mutation assay in mouse lymphoma cells provided an overall negative result for the testes substance.
- Executive summary:
In an mouse lymphoma test (similar to OECD 476) DINA was tested with and without S9-mix in a dose range of 5.6 - 1000 µl/ml. The test substance was dissolved in acetone. There was no evidence of a mammalian cell mutation in the mouse lymphoma assay (McKee, 1985)
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