Chlorosulphuric acid

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro DNA damage and/or repair study

Type of information:

experimental study

Adequacy of study:

weight of evidence

Study period:

Not specified; published in 1988.

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference

Reference Type:

publication

Title:

Unnamed

Year:

1988

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

No guideline was stated in the report, but the study complies with the  TNsG on in vitro gene mutation and is largely compliant with EU Method  B.13/14 and OECD 471.
ce amounts of histidine  and biotin, and then mixed and immediately poured over the surface of  minimal glucose agar plates. The number of revertant colonies was scored  after incubation at 37°C for 48 hours.

GLP compliance:

no

Type of assay:

bacterial reverse mutation assay

Test material

Test material information

Constituent 1

Reference substance name:

Hydrogen chloride

EC Number:

231-595-7

EC Name:

Hydrogen chloride

Cas Number:

7647-01-0

Molecular formula:

Cl H

IUPAC Name:

Hydrogen chloride

Details on test material:

Hydrochloric acid
Specification: Fisher Certified
Purity: 95% or greater
No further details specified.

Method

Species / strain

Species / strain / cell type:

other: Salmonella typhimurium: TA1535, TA1537, TA1538, TA98 and TA100

Details on mammalian cell type (if applicable):

Not specified

Additional strain / cell type characteristics:

not specified

Metabolic activation:

with and without

Metabolic activation system:

S9 derived from liver of rats previously treated with Aroclor 1254.

Test concentrations with justification for top dose:

0.001-5 microlitre/plate

Vehicle / solvent:

no data

Controls

Untreated negative controls:

not specified

Negative solvent / vehicle controls:

yes

Remarks:

Appropriate solvent (absolute ethanol) controls were used, but not reported.

True negative controls:

not specified

Positive controls:

yes

Remarks:

Appropriate positive controls were used, but not reported.

Positive control substance:

not specified

Details on test system and experimental conditions:

Way of application: The agar overlay plate incorporation method of Ames et al. (1975) was used.
Dilutions of the test item with approximately 10^8 cells of the tester strain from an overnight culture and 0.5 mL metabolic activation mixture (replaced with 0.5 mL sterile water for non-activated assay) were combined with 2 mL molten top agar containing trace amounts of histidine and biotin, and then mixed and immediately poured over the surface of minimal glucose agar plates. The number of revertant colonies was scored after incubation at 37 °C for 48 hours.

Pre-incubation time: None.
Other modifications: No
Number of cells evaluated: Approximately 10^8 added to plate.

Evaluation criteria:

The result would be considered positive if the test item produced a positive dose-related response over three concentrations with the lowest increase equal to three times the spontaneous revertant rate for TA1535, TA1537 or TA1538 strains. For TA98 and TA100 strains a doubling of the rate at the highest increase over three dose levels was considered positive.

Statistics:

no data

Results and discussion

Test results

Species / strain:

S. typhimurium TA 1535, TA 1537, TA 98 and TA 100

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

other: Not reported

Vehicle controls validity:

not specified

Untreated negative controls validity:

not specified

Positive controls validity:

not specified

Additional information on results:

Actual concentrations and number of replicates tested are not reported, results of positive controls are not reported, and the test was not repeated.

Any other information on results incl. tables

Negative results were obtained both with and without metabolic activation. 

Applicant's summary and conclusion

Conclusions:

Hydrochloric acid is not mutagenic under these test conditions.