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EC number: 201-963-1 | CAS number: 90-04-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From 23 OCT 1984 to 27 NOV 1984
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Guideline study (OECD 401)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 984
- Report date:
- 1984
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 401 (Acute Oral Toxicity)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method B.1 (Acute Toxicity (Oral))
- Deviations:
- no
- GLP compliance:
- yes
- Test type:
- standard acute method
- Limit test:
- no
Test material
- Reference substance name:
- o-anisidine
- EC Number:
- 201-963-1
- EC Name:
- o-anisidine
- Cas Number:
- 90-04-0
- Molecular formula:
- C7H9NO
- IUPAC Name:
- 2-methoxyaniline
- Details on test material:
- - Name of test material (as cited in study report): Echtrot BB Base flüssig
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Wistar
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Hoechst AG, Kastengrund, SPF-breed
- Weight at study initiation: males mean: 199.4 g; females mean: 188.1 g
- Fasting period before study: 16 h
- Housing: grouped (5 animals per cage) in macrolon cages (type 4) in fully air conditioned rooms
- Diet: rats diet Altromin1324 (Altromin GmbH, Lage/Lippe), ad libitum
- Water: tap water, ad libitum
- Acclimation period: at least 5 days
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 22+/-3
- Humidity (%): 50+/-20
- Photoperiod (hrs dark / hrs light): 12/12
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- other: sesame oil
- Details on oral exposure:
- VEHICLE
- Concentration in vehicle: 25% (w/v)
MAXIMUM DOSE VOLUME APPLIED: 16 ml/kg bw - Doses:
- 1250, 1600, 1800, 2000, 2500, 3150 and 4000 mg/kg bw
(corresponding to 750, 960, 1080, 1200, 1500, 1890 and 2400 mg/kg bw of submission substance only) - No. of animals per sex per dose:
- 5
- Control animals:
- no
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of weighing: weekly
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs
Results and discussion
Effect levelsopen allclose all
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- 1 890 mg/kg bw
- Remarks on result:
- other: (corresponding to 1134 mg/kg bw of submission substance)
- Sex:
- male
- Dose descriptor:
- LD50
- Effect level:
- 1 950 mg/kg bw
- Remarks on result:
- other: (corresponding to 1170 mg/kg bw of submission substance)
- Sex:
- female
- Dose descriptor:
- LD50
- Effect level:
- 1 800 mg/kg bw
- Remarks on result:
- other: (corresponding to 1080 mg/kg bw of submission substance)
- Mortality:
- - deaths occurred in between 20 minutes and about 60 h after the dosage
dosage group [mg/kg bw]: lethality in male animals and lethality in female animals
1250: 0/5 and 0/5
1600: 0/5 and -
1800: 3/5 and 2/5
2000: 3/5 and 4/5
2500: 4/5 and 5/5
3150: - and 5/5
4000: - and 5/5 - Clinical signs:
- other: - lower dose-groups: squatting, staggering gait, reduced spontaneous activity, dizziness and respiratory depression - higher dose-groups: abdominal position, negative righting reflex, orange urine, pale skin and at doses of more than 2500 mg/kg bw, in som
- Gross pathology:
- - congestion of blood vessels in the gastrointestinal tract and lungs, a yellow-red foamy liquid in the intestine, and haemorrhages in stomach, intestine and urinary bladder
- animals sacrificed at the end of the observation period showed no macroscopically visible changes
Applicant's summary and conclusion
- Interpretation of results:
- harmful
- Remarks:
- Migrated information Criteria used for interpretation of results: expert judgment
- Conclusions:
- Single oral application of test material "Echtrot BB Base flüssig" caused lethality in male and female Wistar rats thus resulting in a LD 50 of 1890 mg/kg bw (corresponding to 1134 mg/kg bw submission substance only). The test material contains relevant amounts of the submission substance. Therefore the test results are adequate to fulfil the endpoint requirements.
- Executive summary:
Male and female Wistar rats were subjected to test acute oral toxicity according to a standard acute method (OECD TG 401). The test material "Echtrot BB Base flüssig" was administered by gavage at different doses. Animals showed a range of clinical signs and lethality (combined with visible changes in necropsy) was observed during the 14 days observation period, leading to a LD50 of 1890 mg/kg bw (corresponding to 1134 mg/kg bw submission substance; male and female rats combined).
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