Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 440-850-3 | CAS number: 27311-52-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_ecotoxicological-information.png)
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Study plan 09 November 2000, Final report 27 February 2001
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Guideline study and GLP
Cross-reference
- Reason / purpose for cross-reference:
- reference to other study
Data source
Referenceopen allclose all
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 001
- Report date:
- 2001
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 002
- Report date:
- 2001
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: Appendix to the EU-Guideline 92/69/EWG from 31 July 1992
- Principles of method if other than guideline:
- The Daphnia are exposed to the test substance added to water at a range of concentrations for 48 hours. Under otherwise identical test conditions, and an adequate range of test substance concentrations, different concentrations of a test substance exert different average degrees of effect on the swimming ability of Daphnia. Different concentrations result in different percentages of Daphnia being no longer capable of swimming at the end of the test. The concentrations causing zero or 100% immobilisation are derived directly from the test observations whereas the 48-hour EC50 is determined by calculation if possible.
- GLP compliance:
- yes
Test material
- Reference substance name:
- [TN]Ro 1525[/TN][SPEC][/SPEC][AM]99 %[/AM]
- IUPAC Name:
- [TN]Ro 1525[/TN][SPEC][/SPEC][AM]99 %[/AM]
- Details on test material:
- Active Matter = 98,4 %
Substance-No.: SAT 000926
Batch-No.: GN 1-56 39/62-88
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
Test solutions
- Vehicle:
- no
- Details on test solutions:
- A stock solution of 1 g test material per litre M4-medium was prepared. from this solution a second clear stock solution was prepared by 1:100 dilution. Appropriate volumes were taken from the second solution, tranferred into the test vessels and filled up to about 40 ml with M4-medium. The end volume of 50 ml was adjusted after the transfer of the test organisms into the test vessels.
At the test start the test solutions were clear. With increasing test substance concentration (3 mg/L and more) and with increasing test duration increasing precipitation was observed.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- The test organims were obtained as ephippia in december 2000 from Creasel, 98000 Deinze, Belgium.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
Test conditions
- Hardness:
- Sythetic mineral water M4-medium according to Elendt
- Test temperature:
- 20 °C
- pH:
- test concentration (mg/L)
(nominal) pH
0 8.0
0.1 8.0
0.3 8.0
1 7.9
3 7.9
10 7.9
30 7.8
100 7.8 - Dissolved oxygen:
- test concentration (mg/L)
(nominal) oxygen saturation (%)
0 98
0.1 98
0.3 98
1 98
3 98
10 97
30 97
100 97 - Salinity:
- Sythetic mineral water M4-medium according to Elendt
- Nominal and measured concentrations:
- nominal concentration measured concentration (mg/L)
(mg/L) 0h 24h 48h
1 <1 <1 <1
3 <1 <1 <1
10 <1 <1 <1
30 6.3 <1 <1
100 34.7 32.1 9.9 - Details on test conditions:
- Test vessels 100mL glass beakers, coverd with glass plates
Test volume 50mL
Illumination ~ 900 LUX, 16/8 hours light/dark rhythm
No. of test organisms 20 organisms per concentration in 2 groups of 10
Test duration 48 hours - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 48 h
- Dose descriptor:
- EC0
- Effect conc.:
- 0.1 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC10
- Effect conc.:
- 0.3 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.75 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC100
- Effect conc.:
- 2.95 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- mobility
- Results with reference substance (positive control):
- The 24 hours EC50 of the reference substance potassium dichromate was 1.2 mg/L and thus within the target range of 0.9-1.9 mg/L.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The acute toxicity of Ro 1525 against Daphnia Magna was tested according to EU-Guideline 92/69/EWG of 1992. After 48 hours the effect concentration (EC50) reached 0.75 mg/l related to active substance based on nominal concentration.
- Executive summary:
The acute toxicity of Ro 1525 against Daphnia Magna was tested according to EU-Guideline 92/69/EWG of 1992. After 48 hours the effect concentration (EC50) reached 0.75 mg/l related to active substance based on nominal concentration.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
![ECHA](/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/echa_logo.png)