Methoxyammonium chloride

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

migrated information: read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: GLP guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1992

Report date:

1996

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

O-methylhydroxylamine

EC Number:

200-660-1

EC Name:

O-methylhydroxylamine

Cas Number:

67-62-9

Molecular formula:

CH5NO

IUPAC Name:

O-methylhydroxylamine

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

after 1h only

Test solutions

Vehicle:

yes

Details on test solutions:

Preparation of test solutions / dispersions:
- Aliquot amounts of an aqueous stock solution (1.00 g/ 100 ml) were added to the test water using a glass stick. Subsequently the fish were placed into the aquaria within 20 min after the test material was added.

Test organisms

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: Zebra fish (Danio rerio)
- Source: West Aquarium GmbH, Bad Lauterbach
- Length at study initiation (mean, range): 3.4 cm, 2.0 - 4.0 cm
- Weight at study initiation (mean, range): 0.59 g, 0.39 - 0.76 g
- Feeding during test: Withdrawal of food 1 day before and during exposure

ACCLIMATION
- Acclimation period: about 2 weeks
- Health during acclimation (any mortality observed): 0 %

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test conditions

Hardness:

2.5 mmol CaCO3 / L

Test temperature:

22-23 °C

pH:

8.0 - 8.2

Dissolved oxygen:

7.4 - 8.1 mg/L

Nominal and measured concentrations:

- Nominal test concentrations: 0.464, 1.0, 2.15, 4.64, 10 mg/L
- Initial measured concentrations: n.d., n.d., n.d., 5.4, 11.0 mg/L (detection limit = 5 mg/L)

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Type: open
- Material, size, fill volume: All glass aquaria (30 x 22 x 24 cm), 10 L
- Aeration: Continuous aeration with oil-free air, slight
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- No. of vessels per vehicle control (replicates): 1
- Biomass loading rate: 0.6 g fish/L test water

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Municipal water of the city of Frankenthal, not chlorinated and passed through a charcoal filter, aerated.
- Alkalinity: pH about 8.0
- Ca/Mg ratio: 4 : 1

OTHER TEST CONDITIONS
- Photoperiod: 16 : 8 hours day-night regime

EFFECT PARAMETERS MEASURED:
observations: mortality and symptoms after 0, 4, 24, 48, 72, and 96 h
measurements: pH and oxygen content after 0, 24, 48, 72 and 96 h

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

0.464 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 2

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 0.464 - < 1 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 3

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

1 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

Symptoms:
- Tumbling at a nominal concentration of 2.15 mg/L after 24 hours