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EC number: 230-804-9 | CAS number: 7327-60-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: dermal
Administrative data
- Endpoint:
- acute toxicity: dermal
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1978
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Study appears well conducted with limited reporting of methods and results and a single dose tested only and not a limit dose.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 978
- Report date:
- 1978
Materials and methods
Test guideline
- Guideline:
- other: 16 CFR 1500. 40
- Deviations:
- no
- GLP compliance:
- no
- Test type:
- standard acute method
- Limit test:
- no
Test material
- Reference substance name:
- Nitrilotriacetonitrile
- EC Number:
- 230-804-9
- EC Name:
- Nitrilotriacetonitrile
- Cas Number:
- 7327-60-8
- Molecular formula:
- C6H6N4
- IUPAC Name:
- 2-[bis(cyanomethyl)amino]acetonitrile
- Details on test material:
- purity> 99%
White Crystalline material
Constituent 1
Test animals
- Species:
- rabbit
- Strain:
- other: albino
- Sex:
- not specified
Administration / exposure
- Type of coverage:
- occlusive
- Vehicle:
- unchanged (no vehicle)
- Remarks:
- moistened with saline
- Details on dermal exposure:
- The animals were prepared by clipping the skin of the trunk free of hair. Approximately one-half of the animals was further prepared by making epidermal abrasions every 2 or 3 centimeters longitudinally over the area of exposure. The abrasions were sufficiently deep to penetrate the stratum corneum (horny layer of the epidermis) but not to distrub the derma; that is, not to obtain bleeding.
The sleeve was slipped onto the animal which is then placed in a comfortable but immobilized position in a multiple animal holder. Selected doses of liquids and solutions are introduced under the sleeve. If there is slight leakage from the sleeve, which may occur during the first few hours of exposure, it is collected and reapplied. Dosage levels are adjusted in subsequent exposures (if necessary) to enable a calculation of a dose that would be fatal to 50 percent of the animals. This can be determined from mortality ratios obtained at various doses employed. At the end of 24 hours the sleeves or screens are removed, the volume of unabsorbed material (if any) is measured, and the skin reactions are noted. The subjects are cleaned by thorough wiping, observed for gross symptoms of poisoning, and then observed for 2 weeks.
In this study only one dose level was chosen. - Duration of exposure:
- 24 hours
- Doses:
- 200 mg/kg bw
- No. of animals per sex per dose:
- 10
- Control animals:
- not required
Results and discussion
Effect levels
- Sex:
- not specified
- Dose descriptor:
- LD0
- Effect level:
- 200 mg/kg bw
- Based on:
- test mat.
- Remarks on result:
- other: no mortalities observed
- Mortality:
- No mortality observed
- Clinical signs:
- other: Decreased activity and some nasal discharge was observed, but not indicated in how many animals or at what stage after dosing these effects started and ceased.
- Gross pathology:
- No pathology reported
Applicant's summary and conclusion
- Conclusions:
- A dose of 200 mg/kg was not lethal to adult albino rabbits following occlusive dermal exposure (intact and abraded skin). The LD0, therefore is 200 mg/kg bw.
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