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Diss Factsheets
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EC number: 429-290-0 | CAS number: 3380-30-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic plants other than algae
Administrative data
- Endpoint:
- toxicity to aquatic plants other than algae
- Type of information:
- migrated information: read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: GLP guideline study on Triclosan, suitable for read-across.
Data source
Referenceopen allclose all
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 997
- Report date:
- 1997
- Reference Type:
- other: Amendment
- Title:
- Unnamed
- Year:
- 2 006
- Report date:
- 2006
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- other: EPA, TSCA Environmental Effects Testing Guidelines, Subpart B, Section 797.1050, Federal Register, Vol. 52, No. 97 May 20, 1987;
- Qualifier:
- according to guideline
- Guideline:
- other: ASTM, 1991, Standard guide for conducting static toxicity tests with Lemna gibba G3, E1415-91.
- GLP compliance:
- yes
Test material
- Reference substance name:
- Triclosan
- EC Number:
- 222-182-2
- EC Name:
- Triclosan
- Cas Number:
- 3380-34-5
- IUPAC Name:
- 5-chloro-2-(2,4-dichlorophenoxy)phenol
- Details on test material:
- - Name of test material (as cited in study report): Triclosan
- Lot/batch No.: EN:275927.26
- Analytical purity: 99.5 %
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- yes
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
To begin each test, a fresh primary stock solution was prepared by weighing an appropriate amount of Triclosan and diluting with the solvent acetone. The primary stock solution was mixed by inversion or by sonication for five minutes, resulting in a clear liquid. Secondary stock solutions for each test were prepared, in acetone, by serial dilution at concentrations such that an equivalent amount of solvent was used in each test treatment. The nominal concentration for the primary stock solution was as follows: 1330 µg/mL.
For each toxicity test, the test treatments were prepared by quantitatively diluting aliquots of the appropriate stock solutions, with medium in volumetric flasks, to yield nominal test solution concentrations. Actual nominal concentrations for each definitive test were as follows: 0.500, 2.50, 12.5, and 62.5 µg/L. The no-treatment controls contained medium only. The solvent controls contained an amount of solvent equivalent to the amount present in each test treatment, i.e., 0.5 mL/L. After thorough mixing of the test substance treatments, appropriate volumes of the no-treatment control, solvent control, and each test substance treatment were added to each of the replicate test vessels.
Test organisms
- Test organisms (species):
- Lemna gibba
- Details on test organisms:
- - Common name: Duckweed
- Source: in-house culture
- Age of inoculum (at test initiation): inoculum was taken from healthy 12-d old stock cultures.
- Breeding method: stock cultures were maintained according to facility SOPs. Transfers were made regularly into fresh medium to provide cultures in the logarithmic phase of growth for toxicity test inoculations.
- Culturing conditions: 25±2°C; permanent illumination of 5000 ± 750 lux (warm white lights).
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 7 d
Test conditions
- Test temperature:
- 24.10 - 24.76 °C
- pH:
- start: 7.71 - 7.76
termination: 7.58 - 8.92 - Nominal and measured concentrations:
- The nominal concentrations were 0.5, 2.5, 12.5 and 62.5 µg a.s./L
- Details on test conditions:
- TEST SYSTEM
- Incubation chamber used: yes pH Environmental Incubator, Model CEC-32TC
- Test vessel: 500-mL Erlenmeyer flasks
- Fill volume: 200 ml
- No. of colonies per vessel: Three plants, consisting of four fronds each (total of 12 fronds) per vessel were used
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 3
- No. of vessels per vehicle control (replicates): 3
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Medium was prepared by adding, for each litre, aliquots of the 20X-AAP media stock solution to ASTM Type 1 reagent grade water. The pH was adjusted to 7.5 ± 0.1 with 0.1 N or 1.0 N sodium hydroxide or hydrochloric acid as necessary and the volume brought to one liter. Medium was then filtered through a 0.22 µ filter into a sterile container.
OTHER TEST CONDITIONS
- Photoperiod: Continuous illumination
- Light intensity and quality: 5000 ± 1 5% lux
EFFECT PARAMETERS MEASURED
- Determination of frond number: manual counting
Results and discussion
Effect concentrations
- Duration:
- 7 d
- Dose descriptor:
- EC50
- Effect conc.:
- > 62.5 µg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: reduction in growth (frond number)
- Details on results:
- Exposure of L. gibba to Triclosan resulted in effects ranging from 16.2 % stimulation to 20.9 % inhibition.
Since no test concentration resulted in inhibition > 25 %, the EC50 could not be determined and is reported to be > 62.5 µg/L.
Any other information on results incl. tables
Effects of Triclosan on the growth of Lemna gibba:
Nominal concentration |
Frond counts on day 7 |
% inhibition relative to mean in combined controls |
Combined controls |
163 ± 12 |
- |
0.5 |
178 ± 12 |
- 10.3 |
2.5 |
172 ± 11 |
- 6.08 |
12.5 |
187 ± 5 |
- 16.2 |
62.5 |
131 ± 17 |
20.9 |
A negative percent inhibition indicates stimulation
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.