Benzaldehyde, 3-ethoxy-4-(.beta.-D-glucopyranosyloxy)-

Biodegradation in water: screening tests

Link to relevant study record(s)

Reference

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

July - September 2018

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)

Version / remarks:

17 July 2002

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge (adaptation not specified)

Details on inoculum:

The Secondary effluent of the JRF Sewage Treatment Plan was used as inoculum. The Secondary effluent of a treatment plant was filtered through Whatman No. 1 filter paper, analysed for concentration and found 1.42 x 104 CFU/mL (Bacteria) and 3.00 x 102 CFU/mL (Fungus). Then inoculum was incubated at test temperature 22 +/- 2 °C for 7 days.

Duration of test (contact time):

28 d

Initial conc.:

2.1 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation:

O2 consumption

Details on study design:

TEST CONDITIONS
- Composition of medium: Potassium dihydrogen orthophosphate, Dipotassium hydrogen orthophosphate, Disodium hydrogen orthophosphate dihydrate, Ammonium chloride, Calcium chloride dihydrate, Magnesium sulphate heptahydrate, Iron (III) chloride hexahydrate
- Test temperature: 22 +/- 2 ºC
- pH:

TEST SYSTEM
- Culturing apparatus: BOD Incubator (Sky Lab Instruments & Engineering Pvt. Ltd.)
- Number of culture flasks/concentration: 10 bottles for test suspension, 10 bottles for inoculum blank, 10 bottles for procedure control and 6 bottles for toxicity control
- Method used to create aerobic conditions: The mineral medium was strongly aerated by using an aerator for 20 minutes and then allowed to stand for 20 h at 22 ± 2°C.
- Measuring equipment: Dissolved Oxygen Meter (HACH - USA)

SAMPLING
- Sampling method and frequency: Dissolved oxygen was measured with DO meter on day 7, 14, 21, and 28.

CONTROL AND BLANK SYSTEM
- Inoculum blank: The inoculum blank was prepared by mixing 4.0 mL inoculum with 3996.0 mL mineral medium.
- Toxicity control: The toxicity control was prepared by mixing 2.0 mL stock solution of potassium hydrogen phthalate, 2.0 mL stock solution of test item and 2.0 mL of inoculum to 1994 mL of mineral medium in a conical flask of 2 L capacity. The solution was mixed thoroughly. The final concentration was 2.00 mg/L of reference substance and 2.10 mg/L of test item in mineral medium.

Reference substance:

other: hydrogen phthalate

Key result

Parameter:

% degradation (O2 consumption)

Value:

61.71

Sampling time:

28 d

Key result

Parameter:

% degradation (O2 consumption)

Value:

28.09

Sampling time:

7 d

Validity criteria fulfilled:

yes

Interpretation of results:

readily biodegradable

Conclusions:

Degradation of the test item reached 61.71% on 28 day of analysis, whereas the reference substance exhibited a normal pattern of degradation (up to 83.83%) within 28 days. Hence, the test item was readily biodegradable. The percent degradation in the toxicity control was more than 25% after 14 days. Therefore, the test item is assumingly not inhibitory to microbial activity in the inoculum.

Executive summary:

Degradation of the test item reached 61.71% on 28 day of analysis, whereas the reference substance exhibited a normal pattern of degradation (up to 83.83%) within 28 days. Hence, the test item was readily biodegradable. The percent degradation in the toxicity control was more than 25% after 14 days. Therefore, the test item is assumingly not inhibitory to microbial activity in the inoculum.

Description of key information

Degradation of the test item reached 61.71% on 28 day and 28.09% after 7 days.

Key value for chemical safety assessment

Biodegradation in water:

readily biodegradable

Type of water:

freshwater