1H-Benz[e]indolium, 2-[2-[2-chloro-3-[2-(1,3-dihydro-1,1,3-trimethyl-2H-benz[e]indol-2-ylidene)ethylidene]-1-cyclohexen-1-yl]ethenyl]-1,1,3-trimethyl-, 4-methylbenzenesulfonate (1:1)

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2002

Report date:

2002

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

ISO 8692 (Water Quality - Fresh Water Algal Growth Inhibition Test with Scenedesmus subspicatus and Selenastrum capricornutum)

Test guideline 2

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Alga, Growth Inhibition Test)

Test guideline 3

Qualifier:

according to guideline

Guideline:

EU Method C.3 (Algal Inhibition test)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

-

EC Number:

420-680-6

EC Name:

-

Cas Number:

134127-48-3

Molecular formula:

C47 H47 Cl N2 O3 S

IUPAC Name:

2-{2-[2-chloro-3-(2-{1,3,3-trimethyl-1H,2H,3H-benzo[g]indol-2-ylidene}ethylidene)cyclohex-1-en-1-yl]ethenyl}-1,1,3-trimethyl-1H-benzo[e]indol-3-ium 4-methylbenzene-1-sulfonate

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

at t=0h, t=24h and 72h

Test solutions

Vehicle:

yes

Details on test solutions:

4.6, 10, 22, 46 and 100% of a 5µm filtrate prepared at 100mg/l

Test organisms

Test organisms (species):

Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

72 h

Post exposure observation period:

at t=0h, t=24h and t=72h

Test conditions

Hardness:

24mg CaCO3/l

Test temperature:

23.9 - 24.2°C

pH:

8.5 - 9.0

Nominal and measured concentrations:

Measured concentrations: 0.031mg/l, 0.077mg/l, 0.22mg/l, 0.52mg/l and 1.4mg/l

Results and discussion

Effect concentrations

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

> 0.03 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

dissolved

Basis for effect:

other: growth rate + cell growth

Remarks on result:

other: No EC-values determined, since the apparent effect on cell growth was not considered to be biologically significant, while the effect on growth rate was smaller than 10%

Details on results:

Due to the very low solubility of TRUMP IR DYE in water (0.03mg/l), concentration levels that might be toxic for algae could not be reached. Both for growth inhibition and growth rate reduction, the EC50 is higher than the maximum solubility of TRUMP IR DYE in test medium.

Applicant's summary and conclusion

Validity criteria fulfilled:

no

Remarks:

Exposure concentrations of solution without algae did not remain above 80% relative to initial concentrations (could not be prevented, as no intermittent renewal allowed; decrease of test substance not due to adsorption to algae but due to low solubility)

Conclusions:

not toxic to algae up to water solubility